AMELIORATIVE AND PHYTO-THERAPEUTIC POTENTIAL OF ANCIENT MEDICINAL PLANTS TO MANAGE REPRODUCTIVE HEALTH IN MALE ALBINO RATS

Main Article Content

Mahvish Ajaz
Syed Muhammad Ali Shah
Abid Rashid
Rehana Dilshad
Naveed Munir

Keywords

Nicotine, oxidative stress, infertility, reproductive problems, polyherbal preparation, therapeutic agent

Abstract

The use of nicotine is increasing particularly in young generation which leads to the development of different diseases. Reproductive problem in male and female is also one of them because nicotine directly interferes with antioxidant mechanism, reproductive hormonal axis and destroyed the reproductive organ histology. This research was planned to explore the ameliorative and phytotherapeutic potential of selected medicinal plants including M. pruriens, C. behen and P. embilica hydroethanolic extracts preparation as Polyherbal preparation (PHP) in albino male rats. PHP was evaluated for phytochemicals, antioxidant activities, In-vitro spermatozoa parameters and In-vivo study in male albino rats after induction of infertility by nicotine. The results revealed the presence of wide range phytomolecules including tannins, flavonoids, saponins, alkaloids, triterpenes, and glycosides; TPC (56.13 ± 1.39 mgGAE/g), and TFC (18.915±1.417 μgCE/g dry). HPLC results showed different important phenolic compounds while FTIR analysis reveals many peaks for the presence of functional molecules. The results of hemolytic and thrombolytic activities explored significant (p<0.05) protective role of selected medicinal plants. In-vitro spermatozoa parameters study revealed that PHP has significant (p<0.05) potential on the motility and viability of sperms. The results of in-vivo study explored that PHP treatment significantly (p<0.05) restore the antioxidant status after intoxication with nicotine. A significant (p<0.05) improvement in the hepatic like ALT, AST and renal parameters including urea, creatinine and uric acid was also found. Moreover, the results of reproductive hormones including LH, FSH, testosterone and histological study of testis tissues explored significant (p<0.05) therapeutic impact of PHP treatment as compared to control groups. It could be concluded that selected medicinal plants have significant antioxidant as well as reproductive problems management properties that can help potential uses in traditional medicines as a therapeutic agent. However, trial at high level then clinical trials is required to declare this formation as a safe drug.

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