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Muhammad Ishaque Mujeeb Rehman
Sultan Ayaz
Abid Rashid
Hafiz Muhammad Asif
Seyd Muhammad Ali Shah


Induce hypothyroidism, Polyherbal formulation, Thyroxine


Hypothyroidism is one of the most frequent challenging disorders, with around 1.6 billion people globally at risk. Hypothyroidism affects 9.4% of Pakistan's population, according to research. In this study protective role of poly-herbs (PHFext) again induce hypothyroidism was evaluated. Commiphora wightii, Wathania somnifera, Moringa oleifera, Trigonella foenum graecum, was used in polyherbal formulation. Ethanolic extracts of Guggul (Commiphora wightii), Ashwagandha (Wathania somnifera), Moringa (Moringa oleifera), Fenugreek (Trigonella foenum graecum) was prepared. A total of 18 rabbits was divided into three groups and each group contained 6 rabbits (n=6). Group A was a control group that received a normal diet and mineral water, while Experimental Group Rabbits were divided into two Groups (B and C experimental groups) Experimental group induced with a Propylthiouracil (PTU) (50 mg/kg/day) for 21 days, Group B was given 500 mg/day/oral PHFext and Group C was given Thyroxine 50 mcg/daily for 45 days. Thyroid profile was assessed by using the Mini-VIDAS system. The Thyroid profile was assessed before starting the study, after the induce 21 days, and experimental after 45 days. The result was analyzed using one-way analysis of variance (ANOVA).  The T3 (triiodothyronine), T4 (free thyroxine) was very significantly increase (P<0.001) in the hypothyroidism treatment group (PTU+PHFext) and also showed that the TSH (thyroid stimulating hormone) level was very significantly decreased (P<0.001) in the hypothyroidism treatment group (PTU+PHFext) as compared to the treated group=C (Thyroxine). Histological result showed significant improved in induced animal treated Group=B (PHFext) in Loss of Follicular epithelium, Loss of Colloid, and Congestion of vessels improvement (83.3%).

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1. Negm SHJASEJ. Effect of Soybean, Virgin Coconut and Moringa oleifera Seed Oils on the Propylthiouracil Induced Hypothyroidism in Rats. 2019;40(OCTOBER-DECEMBER):649-59.
2. Naz N, Rizvi S, Sadiq ZJPjops. Assessment of thyroid hormone levels and thyroid disorders: A case study from Gujranwala, Pakistan. 2017;30(4).
3. Mujeeb MI, Abbasi PJRJoP, Sciences P. Comparative Study of Effectiveness and Safety of Herbal Compound Medicine (Constinil) and Allopathic Medicine (Sodium Picosulfate) in Managing Constipation. 2018;6(1):67-75.
4. Shinwari ZK, Qaiser MJPJB. Efforts on conservation and sustainable use of medicinal plants of Pakistan. 2011;43(1):5-10.
5. Khattak SR, Hussain A, Ali AA, Ahmad T, Ayaz S, Akram M, et al. Comparative studies of leaves and bark of Moringa oleifera originated from Khyber Pakhtoon Khwa and Punjab, Pakistan. 2020;9(3):1505-9.
6. Piantanida E, Ippolito S, Gallo D, Masiello E, Premoli P, Cusini C, et al. The interplay between thyroid and liver: implications for clinical practice. 2020;43:885-99.
7. Zahra’a AJJMSCR. Assessment of the impacts of 830 nm Low Power Laser on Triiodothyronine (T3), Thyroxine (T4) and the Thyroid Stimulating Hormone (TSH) in the Rabbits. 2014;2(11):2902-10.
8. Ramesh B, Saralakumari DJJop, biochemistry. Antihyperglycemic, hypolipidemic and antioxidant activities of ethanolic extract of Commiphora mukul gum resin in fructose-fed male Wistar rats. 2012;68:573-82.
9. Gupta A, Gautam MK, Singh RK, Kumar MV, Rao CV, Goel R, et al. Immunomodulatory effect of Moringa oleifera Lam. extract on cyclophosphamide induced toxicity in mice. 2010.
10. Abdel-Wahhab KG, Mourad HH, Mannaa FA, Morsy FA, Hassan LK, Taher RFJMbr. Role of ashwagandha methanolic extract in the regulation of thyroid profile in hypothyroidism modeled rats. 2019;46(4):3637-49.
11. Plants M. Proceeding of. 2010.
12. Devi J, Rajkumar JJAJPP. Antihyperlipidemic effect of ambrex, a polyherbal formulation against experimentally induced hypercholesterolemia in rats. 2013;7:1737-43.
13. Oecd OJAotatcmtn-OfEC-o, Development. Guidelines for the Testing of Chemicals. 2001.
14. Singha MB, Sarma T, Lahkar MJJoP, Phytochemistry. Anti-hypothyroid potential of Bauhinia variegate and Commiphora mukul extracts in albino wistar rats: A histopathological investigation. 2019;8(1):1736-40.
15. Chen W-J, Lin K-H, Lai Y-J, Yang S-H, Pang J-HSJC. Protective effect of propylthiouracil independent of its hypothyroid effect on atherogenesis in cholesterol-fed rabbits: PTEN induction and inhibition of vascular smooth muscle cell proliferation and migration. 2004;110(10):1313-9.
16. S Farrag O, Salman D, Ali FAZ, S Sayed A, Mahmoud ME, A Abd-El Raheem A-ERJJoAVS. Impact of continuous treatment with propylthiouracil on renal and hepatic functions in rabbits. 2020;5(2):49-60.
17. Hossain AOJPRS. Carbimazole and its effects on thyroid gland of female rabbits. 2019;13(3):310.
18. Montgomery DC. Design and analysis of experiments: John wiley & sons; 2017.
19. Saifi N, Jamal YJWJPPS. An analytical study of thyroid hormones in different temperaments (Mizaj). 2016;5:1612-8.
21. Panthi S, Gao TJIJCEM. Diagnosis and management of primary hypothyroidism in Traditional Chinese medicine (TCM) and Traditional Indian Medicine (Ayurveda). 2015;12(009).
22. Bhat SA, Rather SAJJoDD, Therapeutics. Medicinal benefits and scientific justification of Commiphora mukul (Muqil): A review. 2021;11(1-s):170-2.
23. Ojha SK, Nandave M, Arora S, Mehra RD, Joshi S, Narang R, et al. Effect of Commiphora mukul extract on cardiac dysfunction and ventricular function in isoproterenol-induced myocardial infarction. 2008.
24. Stansbury J, Saunders P, Winston DJJoRM. Promoting healthy thyroid function with iodine, bladderwrack, guggul and iris. 2012;1(1):83-90.
25. Jaiswal S, Bara J, Soni R, Saksena PJIJNHS. Medical uses of Commiphora wightii. 2016;5:76-81.
26. Mukherjee PK, Banerjee S, Biswas S, Das B, Kar A, Katiyar CJJoE. Withania somnifera (L.) Dunal-Modern perspectives of an ancient Rasayana from Ayurveda. 2021;264:113157.
27. Sailaja AKJdo. B. Pranaya ragini. Biomedical Applications of Withania Somnifera’’. J Pharmacy and Drug Innovations, 1 (1); DOI: http. 2020.
28. Singh G, Sharma P, Dudhe R, Singh SJABR. Biological activities of Withania somnifera. 2010;1(3):56-63.
29. Kumar R, Nikitha M, Meera V, Umakanth B, Mahesh C, Bharathi D, et al. A pharmacological approaches on moringa oleifera plant-a brief review. 2022:80-5.
30. Alam MA, Quamri MA, Haider NJDm, therapy p. Efficacy and safety of Barg-e-Sahajna (Moringa olifera Lam.) in primary hypothyroidism. 2021.
31. Mondal C, Chandra AKJBJoPS. Goitrogenic/antithyroidal potential of moringa leaves (Moringa oleifera) and spinach (Spinacia oleracea) of Indian origin on thyroid status in male albino rats. 2019;55.
32. Yadav UC, Baquer NZJPb. Pharmacological effects of Trigonella foenum-graecum L. in health and disease. 2014;52(2):243-54.
33. Majumdar J, Chakraborty P, Mitra A, kumar Sarkar N, Sarkar SJE, Endocrinology C, et al. Fenugreek, a potent hypoglycaemic herb can cause central hypothyroidism via leptin–a threat to diabetes phytotherapy. 2017;125(07):441-8.
34. Kiss R, Pesti-Asbóth G, Szarvas MM, Stündl L, Cziáky Z, Hegedűs C, et al. Diosgenin and its fenugreek based biological matrix affect insulin resistance and anabolic hormones in a rat based insulin resistance model. 2019;2019.
35. Al-Zyadi AJJTIJoVM. The therapeutic role of alcoholic extract of fenugreek seeds on hypothyroidism state induced by thiourea and some blood parameters in adult male rabbits: Atyaf JH Al-Zyadi and Jawad K. Arrak. 2015;39(1):1-7.

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