HYPOTHYROIDISM AND HISTOLOGICAL ALTERATION IN INDUCED PTU AND PROTECTIVE ROLE OF PHFEXT

Main Article Content

Muhammad Ishaque Mujeeb Rehman
Sultan Ayaz
Abid Rashid
Hafiz Muhammad Asif
Seyd Muhammad Ali Shah

Keywords

Induce hypothyroidism, Polyherbal formulation, Thyroxine

Abstract

Hypothyroidism is one of the most frequent challenging disorders, with around 1.6 billion people globally at risk. Hypothyroidism affects 9.4% of Pakistan's population, according to research. In this study protective role of poly-herbs (PHFext) again induce hypothyroidism was evaluated. Commiphora wightii, Wathania somnifera, Moringa oleifera, Trigonella foenum graecum, was used in polyherbal formulation. Ethanolic extracts of Guggul (Commiphora wightii), Ashwagandha (Wathania somnifera), Moringa (Moringa oleifera), Fenugreek (Trigonella foenum graecum) was prepared. A total of 18 rabbits was divided into three groups and each group contained 6 rabbits (n=6). Group A was a control group that received a normal diet and mineral water, while Experimental Group Rabbits were divided into two Groups (B and C experimental groups) Experimental group induced with a Propylthiouracil (PTU) (50 mg/kg/day) for 21 days, Group B was given 500 mg/day/oral PHFext and Group C was given Thyroxine 50 mcg/daily for 45 days. Thyroid profile was assessed by using the Mini-VIDAS system. The Thyroid profile was assessed before starting the study, after the induce 21 days, and experimental after 45 days. The result was analyzed using one-way analysis of variance (ANOVA).  The T3 (triiodothyronine), T4 (free thyroxine) was very significantly increase (P<0.001) in the hypothyroidism treatment group (PTU+PHFext) and also showed that the TSH (thyroid stimulating hormone) level was very significantly decreased (P<0.001) in the hypothyroidism treatment group (PTU+PHFext) as compared to the treated group=C (Thyroxine). Histological result showed significant improved in induced animal treated Group=B (PHFext) in Loss of Follicular epithelium, Loss of Colloid, and Congestion of vessels improvement (83.3%).

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