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Muhammad Usman Sarwar
sultan ayaz
Abid Rashid
Syed Muhammad Ali Shah
Muhammad Akram


Antimicrobial, antifungal, Antioxidants, HPLC, Phytochemical, Reactive oxygen species, Salmonella typhi


Medicinal plants contain collections of bioactive compounds like alkaloids, flavonoids, tannins, etc. which are prescribed for the management of acute and chronic diseases. The medicinal plants having bioactive compounds used as therapeutic agents has an important area in biomedical and natural research products. Saussurea lappa is an effective medicinal plant used in traditional medicines to cure the sore throat, tonsilitis, pleurisy, rheumatoid arthritis, typhoid fever and many of bacterial and fungal infections. The extracts and bioactive compounds from Saussurea lappa roots have been evaluated and found to be medicinal importance as an antioxidant, antibacterial and antifungal activities. In this study, the hydro-ethanolic extract of Saussurea lappa was prepared by microwave-assisted extraction (MAE) process. Saussurea lappa HPLC analysis of the chemical compound contained the highest concentration of chlorogenic acid, cinnamic acid, P-coumeric acid, fenalic acid, syringic acid, vanillic acid, sinapic acid, quercetin, gallic acid, caffeic acid, M-coumeric acid concentration ppm. The extract was qualitatively tested for different phytochemicals and the total phenolic content (TPC) of the extract was calculated in terms of gallic acid equivalent (GAE) per gram of dry extract while the contents of flavonoids were determined as quercetin equivalent.

The highest value was 76.14 ± 0.32 and 21.18 ± 0.38 in DPPH and FRAP assay respectively. Antioxidant enzymes SOD, CAT, and POD were also measured as 19.48 ± 0.04, 39.90 ± 0.09, and 4.85 ± 0.04 units/mg respectively. Antimicrobial activity of Saussurea lappa against salmonella typhi strains of bacteria and fungus was also detected against standards. The study results have created an opportunity for researchers and medical practitioners for the treatment of typhoid fever and oxidative stress-related diseases by natural medicines.

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