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Abdul Wadood Chishti
Muhammad Akram
Abid Rashid
Sabira Sultana
Sultan Ayaz


Sessuvium sessuvoides, Phytochemicals, HPLC, antioxidant, hemolytic, anti-mutagenic


Medicinal plants, rich in bioactive compounds, are extensively studied for their therapeutic potential. Sessuvium sessuvoides, a plant from the Cholistan desert, Pakistan, is used in traditional medicine. The aim of present study was to analyze the phytochemical analysis and its antioxidant, cytotoxic and antimutagenic potential of Sessuvium sessuvoides. The quantification of total phenolic and flavonoid content was carried out using the Folin-Ciocalteu and aluminum chloride colorimetric assay respectively. For the identification of phytocompounds, the HPLC was used. DPPH radical scavenging activity was used to determine the antioxidant potential. Cytotoxicity was assessed by RBCs hemolysis and antimutagenic potential was analyzed by Ames assay. Total phenolic compounds in fractions varied widely, ranging from 134.2±4.1 and 279.4±3.1 mg/g expressed as gallic acid equivalents (GAE). SSCE exhibited the highest total phenolic content. The content of flavonoid expressed as catechin equivalents, varied from 208.684±0.4 to 219.737.9±2.2 mg catechin equivalent/g extract. SSCE exhibited the highest total flavonoid content. The HP-LC analysis confirmed the presence of Salicylic acid, Chlorogenic acid, p-coumaric acid, Gallic acid, Caffeic acid, Ferulic acid, Quercetin, hydroxybenzoic acid and Rutin. It exhibits antioxidant activity, with the methanol fraction showing the highest potential. The plant fractions display cytotoxic effects on human erythrocytes, with the ethanol fraction being the least harmful. Notably, all fractions are non-mutagenic, suggesting protective properties against genetic mutations. In conclusion, the study provides compelling evidence of the phytochemical richness of Sessuvium sessuvoides, particularly in terms of phenolic and flavonoid content. This study not only adds to our understanding that Sessuvium sessuvoides has significant antioxidant, antihemolytic and antimutagenic potential. Future research should focus on the identification and isolation of specific bioactive compounds responsible for these health-promoting properties.

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