EXPLORING HPLC ANALYSIS, ANTIOXIDANT POWER, HEMOLYSIS INHIBITION, AND ANTI-MUTAGENIC POTENTIAL IN VARIOUS EXTRACT FRACTIONS OF SESSUVIUM SESSUVOIDES

Main Article Content

Abdul Wadood Chishti
Muhammad Akram
Abid Rashid
Sabira Sultana
Sultan Ayaz

Keywords

Sessuvium sessuvoides, Phytochemicals, HPLC, antioxidant, hemolytic, anti-mutagenic

Abstract

Medicinal plants, rich in bioactive compounds, are extensively studied for their therapeutic potential. Sessuvium sessuvoides, a plant from the Cholistan desert, Pakistan, is used in traditional medicine. The aim of present study was to analyze the phytochemical analysis and its antioxidant, cytotoxic and antimutagenic potential of Sessuvium sessuvoides. The quantification of total phenolic and flavonoid content was carried out using the Folin-Ciocalteu and aluminum chloride colorimetric assay respectively. For the identification of phytocompounds, the HPLC was used. DPPH radical scavenging activity was used to determine the antioxidant potential. Cytotoxicity was assessed by RBCs hemolysis and antimutagenic potential was analyzed by Ames assay. Total phenolic compounds in fractions varied widely, ranging from 134.2±4.1 and 279.4±3.1 mg/g expressed as gallic acid equivalents (GAE). SSCE exhibited the highest total phenolic content. The content of flavonoid expressed as catechin equivalents, varied from 208.684±0.4 to 219.737.9±2.2 mg catechin equivalent/g extract. SSCE exhibited the highest total flavonoid content. The HP-LC analysis confirmed the presence of Salicylic acid, Chlorogenic acid, p-coumaric acid, Gallic acid, Caffeic acid, Ferulic acid, Quercetin, hydroxybenzoic acid and Rutin. It exhibits antioxidant activity, with the methanol fraction showing the highest potential. The plant fractions display cytotoxic effects on human erythrocytes, with the ethanol fraction being the least harmful. Notably, all fractions are non-mutagenic, suggesting protective properties against genetic mutations. In conclusion, the study provides compelling evidence of the phytochemical richness of Sessuvium sessuvoides, particularly in terms of phenolic and flavonoid content. This study not only adds to our understanding that Sessuvium sessuvoides has significant antioxidant, antihemolytic and antimutagenic potential. Future research should focus on the identification and isolation of specific bioactive compounds responsible for these health-promoting properties.

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References

1. ABAD, M. H. K. & NADAF, M. J. J. O. E. O. R. 2023. The ethnobotanical properties and medicinal application of essential oils of Ziziphora persica Bunge from different habitats: A review. 35, 177-196.
2. ABDEL-WAHAB, M., EL-MAHDY, M., ABD-ELLAH, M., HELAL, G., KHALIFA, F. & HAMADA, F. J. P. R. 2003. Influence of p-coumaric acid on doxorubicin-induced oxidative stress in rat’s heart. 48, 461-465.
3. ADARAMOLA, B., ONIGBINDE, A. & SHOKUNBI, O. J. C. I. 2016. Physiochemical properties and antioxidant potential of Persea Americana seed oil. 2, 168-175.
4. ADARAMOLA, B. & ONIGBINDE, A. J. C. I. 2017. Influence of extraction technique on the mineral content and antioxidant capacity of edible oil extracted from ginger rhizome. 3, 1-7.
5. ASIF, M. J. C. I. 2015a. Anti-neuropathic and anticonvulsant activities of various substituted triazoles analogues. 1, 174-183.
6. ASIF, M. J. C. I. 2015b. Anti-tubercular activity of some six membered heterocycle compounds. 1, 134-163.
7. ASIF, M. J. C. I. 2015c. Antiviral and antiparasitic activities of various substituted triazole derivatives: A mini review. 1, 71-80.
8. ASIF, M. J. C. I. 2015d. Chemistry and antioxidant activity of plants containing some phenolic compounds. 1, 35-52.
9. ASIF, M. J. C. I. 2015e. Pharmacologically potentials of different substituted coumarin derivatives. 1, 1-11.
10. AYUB, M. A., HUSSAIN, A. I., HANIF, M. A., CHATHA, S. A. S., KAMAL, G. M., SHAHID, M., JANNEH, O. J. C. & BIODIVERSITY 2017. Variation in phenolic profile, β‐Carotene and flavonoid contents, biological activities of two Tagetes species from Pakistani flora. 14, e1600463.
11. AZEEM, M., HANIF, M., MAHMOOD, K., AMEER, N., CHUGHTAI, F. R. S. & ABID, U. J. P. B. 2023. An insight into anticancer, antioxidant, antimicrobial, antidiabetic and anti-inflammatory effects of quercetin: A review. 80, 241-262.
12. BAI, X., LI, S., LIU, X., AN, H., KANG, X., GUO, S. J. J. O. A. & CHEMISTRY, F. 2022. Caffeic acid, an active ingredient in coffee, combines with DOX for multitarget combination therapy of lung cancer. 70, 8326-8337.
13. BARTWAL, A., MALL, R., LOHANI, P., GURU, S. & ARORA, S. J. J. O. P. G. R. 2013. Role of secondary metabolites and brassinosteroids in plant defense against environmental stresses. 32, 216-232.
14. CAROCHO, M., FERREIRA, I. C. J. F. & TOXICOLOGY, C. 2013. A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. 51, 15-25.
15. CHATHA, S. A. S., HUSSAIN, A. I., ASAD, R., MAJEED, M., ASLAM, N. J. J. O. F. P. & TECHNOLOGY 2014. Bioactive components and antioxidant properties of Terminalia arjuna L. extracts. 5, 1.
16. CHO, J.-Y., MOON, J.-H., SEONG, K.-Y., PARK, K.-H. J. B., BIOTECHNOLOGY, & BIOCHEMISTRY 1998. Antimicrobial activity of 4-hydroxybenzoic acid and trans 4-hydroxycinnamic acid isolated and identified from rice hull. 62, 2273-2276.
17. CHOTIMARKORN, C. & USHIO, H. J. P. 2008. The effect of trans-ferulic acid and gamma-oryzanol on ethanol-induced liver injury in C57BL mouse. 15, 951-958.
18. DEWANTO, V., WU, X., ADOM, K. K., LIU, R. H. J. J. O. A. & CHEMISTRY, F. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. 50, 3010-3014.
19. EL SHAER, H., ATTIA-ISMAIL, S. J. H., SALT-TOLERANT FEEDSTUFFS IMPACT ON NUTRITION, P., TAYLOR, R. O. L. B. R. C. P. & GROUP, F. 2015. Halophytic and salt tolerant feedstuffs in the Mediterranean basin and Arab region: an overview. 21-36.
20. FLOWERS, T. J. & COLMER, T. D. J. A. O. B. 2015. Plant salt tolerance: adaptations in halophytes. 115, 327-331.
21. FORMULARY, W. W. M. J. W. H. O. G., SWITZERLAND 2009. Stuart, MC, Kouimtzi, M., Hill, SR, Eds.
22. FÜRSTENBERG-HÄGG, J., ZAGROBELNY, M. & BAK, S. J. I. J. O. M. S. 2013. Plant defense against insect herbivores. 14, 10242-10297.
23. GUPTA, A. K. J. Q. S. O. I. M. P. V. 2003. Quality standards of Indian medicinal plants. Volume 1.
24. HAMID, A. A., OGUNTOYE, S. O., ALLI, S. O., AKOMOLAFE, G. A., ADERINTO, A., OTITIGBE, A., OGUNDARE, A. M., ESINNIOBIWA, Q. M. & AMINU, R. O. J. C. I. 2016. Chemical composition, antimicrobial and free radical scavenging activities of Grewia pubescens. 2, 254-261.
25. HASSANEIN, E. H., ABDEL-WAHAB, B. A., ALI, F. E., ABD EL-GHAFAR, O. A., KOZMAN, M. R., SHARKAWI, S. M. J. E. S. & RESEARCH, P. 2021. Trans-ferulic acid ameliorates cisplatin-induced testicular damage via suppression of TLR4, P38-MAPK, and ERK1/2 signaling pathways. 28, 41948-41964.
26. HELMY, S. A., EL-MOFTY, S., EL GAYAR, A. M., EL-SHERBINY, I. M., YOUSRA, M. J. B. & PHARMACOTHERAPY 2022. Novel doxorubicin/folate-targeted trans-ferulic acid-loaded PLGA nanoparticles combination: In-vivo superiority over standard chemotherapeutic regimen for breast cancer treatment. 145, 112376.
27. HUDSON, E. A., DINH, P. A., KOKUBUN, T., SIMMONDS, M. S., GESCHER, A. J. C. E. B. & PREVENTION 2000. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. 9, 1163-1170.
28. IQBAL, M., NISAR, J., ADIL, M., ABBAS, M., RIAZ, M., TAHIR, M. A., YOUNUS, M. & SHAHID, M. J. C. 2017. Mutagenicity and cytotoxicity evaluation of photo-catalytically treated petroleum refinery wastewater using an array of bioassays. 168, 590-598.
29. JIMÉNEZ-BONILLA, P., ZHANG, J., WANG, Y., BLERSCH, D., DE-BASHAN, L.-E., GUO, L. & WANG, Y. J. B. T. 2020. Enhancing the tolerance of Clostridium saccharoperbutylacetonicum to lignocellulosic-biomass-derived inhibitors for efficient biobutanol production by overexpressing efflux pumps genes from Pseudomonas putida. 312, 123532.
30. KAHKESHANI, N., FARZAEI, F., FOTOUHI, M., ALAVI, S. S., BAHRAMSOLTANI, R., NASERI, R., MOMTAZ, S., ABBASABADI, Z., RAHIMI, R. & FARZAEI, M. H. J. I. J. O. B. M. S. 2019. Pharmacological effects of gallic acid in health and diseases: A mechanistic review. 22, 225.
31. KRISHNAIAH, D., SARBATLY, R., NITHYANANDAM, R. J. F. & PROCESSING, B. 2011. A review of the antioxidant potential of medicinal plant species. 89, 217-233.
32. KUMAR, S., NARWAL, S., KUMAR, V. & PRAKASH, O. J. P. R. 2011. α-glucosidase inhibitors from plants: A natural approach to treat diabetes. 5, 19.
33. LIN, D., XIAO, M., ZHAO, J., LI, Z., XING, B., LI, X., KONG, M., LI, L., ZHANG, Q. & LIU, Y. J. M. 2016. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. 21, 1374.
34. LODOVICI, M., CALDINI, S., MORBIDELLI, L., AKPAN, V., ZICHE, M. & DOLARA, P. J. T. 2009. Protective effect of 4-coumaric acid from UVB ray damage in the rabbit eye. 255, 1-5.
35. MERROUNI, A. J. J. M. E. S., 14 , 153 I., Elachouri. M.(2023) NEMMAP Database: A platform for exploring the Ethnobotanical, Pharmacological, and Phytochemical profile of North-Eastern Morocco Medicinal plants. 160.
36. MRADU, G., SAUMYAKANTI, S., SOHINI, M., ARUP, M. J. I. J. O. P. & RESEARCH, P. 2012. HPLC profiles of standard phenolic compounds present in medicinal plants. 4, 162-167.
37. NASEEM, Z., ZAHID, M., HANIF, M. A. & SHAHID, M. J. P. J. O. E. S. 2020. Environmentally friendly extraction of bioactive compounds from Mentha arvensis using deep eutectic solvent as green extraction media. 29, 3749-3757.
38. NAVEED, M., HEJAZI, V., ABBAS, M., KAMBOH, A. A., KHAN, G. J., SHUMZAID, M., AHMAD, F., BABAZADEH, D., FANGFANG, X., MODARRESI-GHAZANI, F. J. B. & PHARMACOTHERAPY 2018. Chlorogenic acid (CGA): A pharmacological review and call for further research. 97, 67-74.
39. NAZ, S., HANIF, M., BHATTI, H. & SHAHID, M. J. O. C. 2016. Partition, fractionation, antioxidant potential and phenolics profiling of Cannabis sativa growing in Pakistan. 39, 2946-2960.
40. ORGANIZATION, W. H. 2013. WHO traditional medicine strategy: 2014-2023, World Health Organization.
41. PRAGASAM, S. J., MURUNIKKARA, V., SABINA, E. P. & RASOOL, M. J. R. I. 2013. Ameliorative effect of p-coumaric acid, a common dietary phenol, on adjuvant-induced arthritis in rats. 33, 325-334.
42. REHMAN, F.-U., ADEEL, S., SHAHID, M., BHATTI, I. A., NASIR, F., AKHTAR, N., AHMAD, Z. J. R. P. & CHEMISTRY 2013. Dyeing of γ-irradiated cotton with natural flavonoid dye extracted from irradiated onion shells (Allium cepa) powder. 92, 71-75.
43. REZAEIROSHAN, A., SAEEDI, M., MORTEZA-SEMNANI, K., AKBARI, J., HEDAYATIZADEH-OMRAN, A., GOLI, H. & NOKHODCHI, A. J. J. O. P. I. 2022. Vesicular formation of trans-ferulic acid: an efficient approach to improve the radical scavenging and antimicrobial properties. 1-10.
44. RUBAB, K., ABBASI, M. A., SIDDIQUI, S. Z., SHAH, S. A. A., ASHRAF, M., AHMAD, I., LODHI, M. A., GHUFRAN, M., SHAHID, M. & FATIMA, H. J. P. J. O. P. S. 2017. Synthesis, pharmacological screening and computational analysis of some 2-(1H-Indol-3-yl)-N'-[(un) substituted phenylmethylidene] acetohydrazides and 2-(1H-Indol-3-yl)-N'-[(un) substituted benzoyl/2-thienylcarbonyl] acetohydrazides. 30, 1263-1275.
45. SAJID-UR-REHMAN, M., ISHTIAQ, S., KHAN, M. A., ALSHAMRANI, M., YOUNUS, M., SHAHEEN, G., ABDULLAH, M., SARWAR, G., KHAN, M. S. & JAVED, F. J. I. 2021. Phytochemical profiling, in vitro and in vivo anti-inflammatory, analgesic and antipyretic potential of Sesuvium sesuvioides (Fenzl) Verdc.(Aizoaceae). 29, 789-800.
46. SATO, Y., ITAGAKI, S., KUROKAWA, T., OGURA, J., KOBAYASHI, M., HIRANO, T., SUGAWARA, M. & ISEKI, K. J. I. J. O. P. 2011. In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. 403, 136-138.
47. SHAHZADI, T., ZAIB, M., RIAZ, T., SHEHZADI, S., ABBASI, M. A., SHAHID, M. J. A. J. F. S. & ENGINEERING 2019. Synthesis of eco-friendly cobalt nanoparticles using Celosia argentea plant extract and their efficacy studies as antioxidant, antibacterial, hemolytic and catalytical agent. 44, 6435-6444.
48. SHARIF, S., RASHID, S., ATTA, A., IRSHAD, A., RIAZ, M., SHAHID, M., MUSTAFA, G. J. J. O. B. R. & AGENTS, H. 2018. Phenolics, tocopherols and fatty acid profiling of wild and commercial mushrooms from Pakistan. 32, 863-867.
49. SHAYAN-NASR, M., GHANIEI, A., ESLAMI, M. & ZADEH-HASHEM, E. J. P. S. 2021. Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by β-cyfluthrin during low temperature liquid storage. 100, 101308.
50. SHIVASHANKARA, R., HANIADKA, R., FAYAD, P., PALATTY, R., ARORA, M., BALIGA, A. J. W., RR, PREEDY, VRBT-BF AS DI FOR L. & GD , A. P., SAN DIEGO 2013. Chapter 42—Hepatoprotective Effects of Zingiber officinale Roscoe (Ginger): A Review. 657-671.
51. SINGH, S. S. & PATIL, K. N. J. J. O. F. B. 2022. trans‐ferulic acid attenuates hyperglycemia‐induced oxidative stress and modulates glucose metabolism by activating AMPK signaling pathway in vitro. 46, e14038.
52. TROMBINO, S., CASSANO, R., BLOISE, E., MUZZALUPO, R., TAVANO, L. & PICCI, N. J. C. P. 2009. Synthesis and antioxidant activity evaluation of a novel cellulose hydrogel containing trans-ferulic acid. 75, 184-188.
53. TROMBINO, S., CASSANO, R., FERRARELLI, T., BARONE, E., PICCI, N., MANCUSO, C. J. C. & BIOINTERFACES, S. B. 2013. Trans-ferulic acid-based solid lipid nanoparticles and their antioxidant effect in rat brain microsomes. 109, 273-279.
54. VAUZOUR, D., CORONA, G., SPENCER, J. P. J. A. O. B. & BIOPHYSICS 2010. Caffeic acid, tyrosol and p-coumaric acid are potent inhibitors of 5-S-cysteinyl-dopamine induced neurotoxicity. 501, 106-111.
55. WANG, S., SUH, J. H., ZHENG, X., WANG, Y., HO, C.-T. J. J. O. A. & CHEMISTRY, F. 2017. Identification and quantification of potential anti-inflammatory hydroxycinnamic acid amides from wolfberry. 65, 364-372.
56. WHALEY, A., IVKIN, D., ZHAPARKULOVA, K., OLUSHEVA, I., SEREBRYAKOV, E., SMIRNOV, S., SEMIVELICHENKO, E., GRISHINA, A. Y., KARPOV, A. & ELETCKAYA, E. J. J. O. E. 2023. Chemical composition and cardiotropic activity of Ziziphora clinopodioides subsp. bungeana (Juz.) Rech. f. 116660.
57. YANG, L., GAO, Y., BAJPAI, V. K., EL-KAMMAR, H. A., SIMAL-GANDARA, J., CAO, H., CHENG, K.-W., WANG, M., ARROO, R. R., ZOU, L. J. C. R. I. F. S. & NUTRITION 2023. Advance toward isolation, extraction, metabolism and health benefits of kaempferol, a major dietary flavonoid with future perspectives. 63, 2773-2789.
58. ZAFEER, M. F., FIRDAUS, F., ANIS, E. & HOSSAIN, M. M. J. N. 2019. Prolong treatment with Trans-ferulic acid mitigates bioenergetics loss and restores mitochondrial dynamics in streptozotocin-induced sporadic dementia of Alzheimer's type. 73, 246-257.
59. ZANG, L.-Y., COSMA, G., GARDNER, H., SHI, X., CASTRANOVA, V. & VALLYATHAN, V. J. A. J. O. P.-C. P. 2000. Effect of antioxidant protection by p-coumaric acid on low-density lipoprotein cholesterol oxidation. 279, C954-C960.

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