An in vitro analysis of the antioxidant and anti-inflammatory effects of Total-etch dentin adhesive containing Hesperidin

Main Article Content

M. Shamly
Iffat Nasim
Krishnakanth Jaju


Hesperidin, Collagen-crosslinker, Total etch dentin adhesive, Micro-organisms, Quality of life


Background: Antioxidants help to protect materials from oxidative damage, which can lead to degradation and discoloration over time. Anti-inflammatory agents can help to reduce inflammation in the oral tissues, which can promote healing and reduce the risk of complications. Hesperidin is a flavonoid compound that is primarily found in citrus fruits, especially in the peels and pulp of oranges and lemons. It has been studied extensively for its potential health benefits, including its antioxidant and anti-inflammatory properties.
Materials And Methods: 2% of Hesperidin was incorporated into total etch adhesive. Anti oxidant property was assessed using DPPH assay and Anti inflammatory property was assessed using BSA assay. Ascorbic acid and Aspirin were used a standard to test anti-oxidant and anti-inflammatory activities respectively. Statistical analysis was performed using independent sample t test.
Results: There was no statistical difference between the groups. In the current investigation, the test groups displayed antioxidant and anti-inflammatory values that were very similar to the tested standards.
Conclusion: In conclusion, Hesperidin-incorporated total etch adhesive offers anti-oxidant and anti-inflammatory properties that may help to increase the stability of the hybrid layer at the tooth restoration interface.
Clinical Significance: Incorporation of hesperidin into bonding agents may offer potential health benefits to patients undergoing restorative dental procedures.

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