Antimicrobial property and Cytotoxicity of hesperidin incorporated dentin adhesive – an invitro study

Main Article Content

Iffat Nasim


Hesperidin, Flavonoid, Total etch dentin adhesive, Micro-organisms, Quality of life


Introduction: Dentin adhesives are frequently used in restorative dentistry, although they can be vulnerable to bacterial colonisation, which can result in recurrent caries. Citrus fruits contain the flavonoid hesperidin (HPN), which has demonstrated potential antibacterial activity against a variety of microorganisms. This study looked at the cytotoxicity and antibacterial effects of total etch dentin adhesive that was incorporated with hesperidin.
Materials and Methods: Four groups of dentin adhesive formulations with different hesperidin concentrations (25μL, 50μL, and 100μL) were made. The test microbes for the antimicrobial investigation were Lactobacillus acidophilus, Streptococcus mutans, and Enterococcus faecalis. For this experiment, Mueller Hinton Agar was used to measure the zone of inhibition. Also, the cytotoxicity of the hesperidin-incorporated total etch dentin adhesive was examined using the Brine Shrimp Lethality Assay.
Results: Dimethyl sulfoxide (DMSO), total etch bonding agent, and HPN work together synergistically to combat L. acidophilus, E. faecalis, and S. mutans in particular. Hesperidin concentration in the adhesive led to an expansion of the zone of inhibition. The hesperidin-incorporated adhesive did not have any harmful effects, as shown by the Brine shrimp lethality experiment.
Conclusion: According to the results of this in vitro investigation, adding hesperidin to dentin adhesive may improve its antibacterial capabilities without harming dental pulp stem cells. These findings open up new directions for investigation into the development of dental adhesive formulations with enhanced antibacterial characteristics for superior clinical results.
Clinical Significance: Hesperidin and other natural substances could be used into dentin adhesive to prevent bacterial colonisation and avoid recurrent caries. Furthermore, the hesperidin-incorporated adhesive's lack of cytotoxicity proves that it is safe for usage in clinical settings.

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