Evaluation of wettability of hesperidin incorporated dentin adhesive -an invitro study

Main Article Content

M. Shamly
Iffat Nasim

Keywords

Hesperidin, Flavonoid, Dentin bonding agent, Micro-organisms, Quality of life

Abstract

Introduction: Measuring the contact angle of a dental adhesive on dentin can provide valuable information about its wettability and bonding potential. A lower contact angle indicates that the adhesive has better wettability and is more likely to form a strong and durable bond with the dentin. Collagen fibres become gelatinized as a result of acid etching, which inhibits resin diffusion in interfibrillar gaps. As a result collagen fibres that aren't protected can then degrade. This degradation can be avoided by adding substances with collagen crosslinking and MMP inhibitory properties to total etch dentin adhesive.
Materials and methods: The commercially available Adper single bond 2 total etch adhesive was used as a parent material. Control group serves the plain adper single bond 2 and the experimental group was prepared by adding 2% of Hesperidin diluted in Dimethyl sulfoxide to adper single bond 2 . Slices of labial enamel were made using a hard tissue microtome. A micro-syringe was used to apply experimental and control total etch adhesives over a sectioned tooth specimen in order to measure the contact angle of the bonding agent with the tooth specimen using Ossila Goniometer. The results were subjected to SPSS software 23. Student independent t test was done to determine the significant difference between groups.
Results: The mean value of experimental group is 30.80 with a standard deviation of 3.389 and the mean value of control group is 37.02 with a standard deviation of 5.523 with p value 0.242 and 95% confidence interval. The mean values of the experimental group were lower when compared to the control group. This implies that the wettability of test group is better than the control group. However the results are statistically insignificant because of small sample size.
Conclusion: Hesperidin together with Dimethyl sulfoxide synergistically shows improved wettability of total etch adhesive compared to the control group.
Clinical Significance: Total etch dentin adhesive with hesperidin incorporation can reduce the risk of post-operative sensitivity, inhibit MMP activity, and offer a natural substitute for synthetic additives while also enhancing the bond strength between the composite restoration and the tooth. 

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