Spectrophotometric colour stability analysis of Hesperidin incorporated Total-etch dentin adhesive- an invitro study

Main Article Content

M. Shamly
Iffat Nasim

Keywords

Hesperidin, Collagen-crosslinker, Total etch dentin adhesive, Micro-organisms, Quality of life

Abstract

Background: Hesperidin has been investigated for its ability to promote collagen cross-linking, enhancing the stability and mechanical properties of collagen fibers. By incorporating hesperidin into the total-etch dentin adhesive, it is believed that it may contribute to improved bonding and longevity by strengthening the collagen matrix and reducing the risk of collagen degradation. The rationale behind incorporating these additives is to potentially enhance adhesive properties, improve bonding effectiveness, and address specific challenges related to color stability and moisture control.
Materials and methods: In this study, the total etch adhesive used was Adper single bond 2 (3m ESPE). 2% Hesperidin dissolved in Dimethyl sulphoxide was incorporated into total etch adhesive. Color stability was checked using Spectrophotometer after polymerisation of adhesives using a glass slide mould. The initial color of the specimens were measured according to the CIELAB color system. The L*, a*, and b* values were recorded before and after ageing process at 7 days and 1 month. Color difference DE* was calculated using the following equation: DE*=[DL* 2 + Da* 2 + Db* 2 ]1/2.
Results: The color difference values for control group at 7 days and 1 month were 0.18 and 2.12 respectively and for the test group were 5.96 and 7.49 respectively. Color instability was more in test group compared to the control group. The colour difference is often not perceived visually and is regarded as clinically appropriate if the computed E value is less than 10. Thus the test group values are still clinically acceptable.
Conclusion: Overall, the findings of this study suggest that hesperidin can be a promising natural additive for improving the bonding ability of dentin adhesive without compromising its color stability. However, further studies are needed to evaluate the long-term durability and clinical efficacy of the hesperidin-incorporated dentin adhesive in vivo.
Clinical Significance: Adding Hesperidin as a natural collagen cross linker to total-etch dentin adhesives may benefit in improving the longevity of composite restorations by stabilising the dentin hybrid layer without compromising the color stability.

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References

1. Tang C, Ahmed MH, Yao C, et al. Bonding performance of experimental HEMA-free two-step universal adhesives to low C-factor flat dentin. Dent Mater. Epub ahead of print 8 May 2023. DOI: 10.1016/j.dental.2023.04.008.
2. Douglas RD. Color stability of new-generation indirect resins for prosthodontic application. J Prosthet Dent 2000; 83: 166–170.
3. Benetti AR, Ribeiro de Jesus VCB, Martinelli NL, et al. Colour stability, staining and roughness of silorane after prolonged chemical challenges. J Dent 2013; 41: 1229–1235.
4. Caldas IP, da Silva EM, Lourenço ES, et al. The influence of methodology on the comparison of cytotoxicity of total-etch and self-etch adhesive systems. J Dent 2022; 122: 104158.
5. Fang K, Chen K, Shi M, et al. Effect of different adhesive systems on dental defects and sensitivity to teeth in composite resin restoration: a systematic review and meta-analysis. Clin Oral Investig. Epub ahead of print 5 April 2023. DOI: 10.1007/s00784-023-05007-0.
6. Levartovsky S, Ferdman B, Safadi N, et al. Effect of Silica-Modified Aluminum Oxide Abrasion on Adhesion to Dentin, Using Total-Etch and Self-Etch Systems. Polymers ; 15. Epub ahead of print 14 January 2023. DOI: 10.3390/polym15020446.
7. Hardan L, Daood U, Bourgi R, et al. Effect of Collagen Crosslinkers on Dentin Bond Strength of Adhesive Systems: A Systematic Review and Meta-Analysis. Cells; 11. Epub ahead of print 4 August 2022. DOI: 10.3390/cells11152417.
8. Alsaeed AY. Bonding CAD/CAM materials with current adhesive systems: An overview. Saudi Dent J 2022; 34: 259–269.
9. Mosharrafian S, Farahmand N, Poorzandpoush K, et al. In vitro microleakage at the enamel and dentin margins of class II cavities of primary molars restored with a bulk-fill and a conventional composite. Clin Exp Dent Res. Epub ahead of print 29 March 2023. DOI: 10.1002/cre2.729.
10. Ashy LM, Marghalani H, Silikas N. In Vitro Evaluation of Marginal and Internal Adaptations of Ceramic Inlay Restorations Associated with Immediate vs Delayed Dentin Sealing Techniques. Int J Prosthodont 2020; 33: 48–55.
11. Liu H, Guo J, Wang R, et al. Theaflavins as a novel cross-linker quickly stabilize demineralized dentin collagen against degradation. Sci Rep 2021; 11: 19699.
12. Hass V, Li Y, Wang R, et al. Methacrylate-functionalized proanthocyanidins as novel polymerizable collagen cross-linkers - Part 1: Efficacy in dentin collagen bio-stabilization and cross-linking. Dent Mater 2021; 37: 1183–1192.
13. Jain K, Beri L, Kunjir K, et al. Comparative evaluation of the effect of 10% sodium ascorbate, 10% hesperidin, 1% riboflavin 5 phosphate, collagen cross-linkers, on the pushout bond strength of fiber postluted to radicular dentin: study. J Conserv Dent 2018; 21: 95–99.
14. Malli Sureshbabu N, Selvarasu K, V JK, et al. Concentrated Growth Factors as an Ingenious Biomaterial in Regeneration of Bony Defects after Periapical Surgery: A Report of Two Cases. Case Rep Dent 2019; 2019: 7046203.
15. Ahad M, Gheena S. Awareness, attitude and knowledge about evidence based dentistry among the dental practitioner in Chennai city. J Adv Pharm Technol Res 2016; 9: 1863.
16. PradeepKumar AR, Shemesh H, Jothilatha S, et al. Diagnosis of Vertical Root Fractures in Restored Endodontically Treated Teeth: A Time-dependent Retrospective Cohort Study. J Endod 2016; 42: 1175–1180.
17. Jangid K, Alexander AJ, Jayakumar ND, et al. Ankyloglossia with cleft lip: A rare case report. J Indian Soc Periodontol 2015; 19: 690–693.
18. Kumar A, Sherlin HJ, Ramani P, et al. Expression of CD 68, CD 45 and human leukocyte antigen-DR in central and peripheral giant cell granuloma, giant cell tumor of long bones, and tuberculous granuloma: An immunohistochemical study. Indian J Dent Res 2015; 26: 295–303.
19. Manohar J, Abilasha R. A Study on the Knowledge of Causes and Prevalance of Pigmentation of Gingiva among Dental Students. Indian Journal of Public Health Research & Development 2019; 10: 95.
20. Sekar D, Mani P, Biruntha M, et al. Dissecting the functional role of microRNA 21 in osteosarcoma. Cancer Gene Ther 2019; 26: 179–182.
21. Girija SA, Jayaseelan VP, Arumugam P. Prevalence of VIM- and GIM-producing Acinetobacter baumannii from patients with severe urinary tract infection. Acta Microbiol Immunol Hung 2018; 65: 539–550.
22. Maheswari TNU, Venugopal A, Sureshbabu NM, et al. Salivary micro RNA as a potential biomarker in oral potentially malignant disorders: A systematic review. Ci Ji Yi Xue Za Zhi 2018; 30: 55–60.
23. Subashri A, Maheshwari TNU. Knowledge and attitude of oral hygiene practice among dental students. J Adv Pharm Technol Res 2016; 9: 1840.
24. Sridharan G, Ramani P, Patankar S, et al. Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma. J Oral Pathol Med 2019; 48: 299–306.
25. Ezhilarasan D, Apoorva VS, Ashok Vardhan N. Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells. J Oral Pathol Med 2019; 48: 115–121.
26. Mathew MG, Samuel SR, Soni AJ, et al. Evaluation of adhesion of Streptococcus mutans, plaque accumulation on zirconia and stainless steel crowns, and surrounding gingival inflammation in primary molars: randomized controlled trial. Clin Oral Investig 2020; 24: 3275–3280.
27. Vijayashree Priyadharsini J. In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens. J Periodontol 2019; 90: 1441–1448.
28. Chandrasekar R, Chandrasekhar S, Sundari KKS, et al. Development and validation of a formula for objective assessment of cervical vertebral bone age. Prog Orthod 2020; 21: 38.
29. Azevedo GS. The Effect of Composite Resin Polymerization Shrinkage on Dentin-composite Resin Microtensile Bond Strength. 2000.
30. Lempel E, Szebeni D, Őri Z, et al. The effect of high-irradiance rapid polymerization on degree of conversion, monomer elution, polymerization shrinkage and porosity of bulk-fill resin composites. Dent Mater 2023; 39: 442–453.
31. Albers HF. Tooth-colored Restoratives: Principles and Techniques. PMPH-USA, 2002.
32. Nakabayashi N, Pashley DH. Hybridization of Dental Hard Tissues. Quintessence Publishing (IL), 1998.
33. Srinivasulu S, Vidhya S, Sujatha M, et al. Shear bond strength of composite to deep dentin after treatment with two different collagen cross-linking agents at varying time intervals. Oper Dent 2012; 37: 485–491.
34. Extracellular Matrix Proteins—Advances in Research and Application: 2013 Edition. ScholarlyEditions, 2013.
35. Cayetano-Salazar L, Nava-Tapia DA, Astudillo-Justo KD, et al. Flavonoids as regulators of TIMPs expression in cancer: Consequences, opportunities, and challenges. Life Sci 2022; 308: 120932.
36. Braga GP de A, Caiaffa KS, Pereira JA, et al. Microbiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy. J Funct Biomater; 13. Epub ahead of print 17 December 2022. DOI: 10.3390/jfb13040305.
37. Yan L, Zhang S, Zhou X, et al. Anti-biofilm and bacteriostatic effects of three flavonoid compounds on. Biofouling 2023; 1–12.
38. Yamane K, Kato Y. Handbook on Flavonoids: Dietary Sources, Properties and Health Benefits. Nova Science Publishers, 2012.
39. Puri M. Food Bioactives: Extraction and Biotechnology Applications. Springer, 2017.
40. Nagpal M, Taneja S, Kumar M, et al. Evaluation of the effect of natural versus synthetic matrix metalloproteinase silencers in preservation of dentin collagen and long-term bond strength of total etch adhesive. J Conserv Dent 2020; 23: 221–226.
41. Islam MS, Hiraishi N, Nassar M, et al. Effect of hesperidin incorporation into a self-etching primer on durability of dentin bond. Dent Mater 2014; 30: 1205–1212.
42. Zhang M, Sun Z, Zhang B, et al. Effects of dimethyl sulfoxide pretreatment on the bonding properties of fluorotic dentin of different severity: An in vitro study. J Prosthet Dent. Epub ahead of print 9 March 2022. DOI: 10.1016/j.prosdent.2022.01.033.