ANTIANXIETY POTENTIAL AND ACUTE TOXICITY ASSESSMENT OF FRAGARIA ANANASSA FRUIT AND LEAVES EXTRACT
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Keywords
Abstract
Background: Anxiety is life threatening and uncontrollable phobia with a wide range of symptoms and marked number of causes. A variety of pharmacologic agents are used to treat anxiety and depression but the potential side effects lead to the search from newer agents with fewer side effects. Pharmacologist add natural plant-based components to treatments as supplements. The Fragaria Ananassa plant has therapeutic significance and is being employed in various ways.
Objective: The purpose of this study was to determine whether three different doses of fruit and leaves extract of Fragaria Ananassa at different time intervals had any antianxiety effects and to also assess its toxicity index.
Methods: This study was carried out in forty-eight Swiss albino mice (20–30 g of 6–8 weeks). Anxiolytic effect was determined by open field test at three different doses of ethanolic extract of Fragaria Ananassa fruit extract (FE) and leaves extract (LE) (i.e. 100mg/kg, 300mg/kg and 500mg/kg). The specific parameters measured in open field test (OFT) which indicate the spontaneous behavior of the animal. Acute toxicity test was done described by lorke. A p-value of <0.05 was considered statistically significant.
Objectives: This study intended to assess the anxiolytic properties and safety of Fragaria ananassa fruit and leaves extracts in Swiss albino mice through the Open Field Test and acute toxicity evaluation.
Results: Different doses of Fragaria Ananassa fruit extract and leaves extract have shown statistically insignificant antianxiety effect. However clinically significant improvement on different anxiety parameters in mice is observed with different doses of fruit and leaves extracts. There was no toxicity or mortality observed during the acute toxicity study.
Conclusion: Using OFT, clinically significant improvement in anxiety is observed with strawberry fruit and leaves extracts and its LD50 is greater than 5000mg/kg.
Objective: The purpose of this study was to determine whether three different doses of fruit and leaves extract of Fragaria Ananassa at different time intervals had any antianxiety effects and to also assess its toxicity index.
Methods: This study was carried out in forty-eight Swiss albino mice (20–30 g of 6–8 weeks). Anxiolytic effect was determined by open field test at three different doses of ethanolic extract of Fragaria Ananassa fruit extract (FE) and leaves extract (LE) (i.e. 100mg/kg, 300mg/kg and 500mg/kg). The specific parameters measured in open field test (OFT) which indicate the spontaneous behavior of the animal. Acute toxicity test was done described by lorke. A p-value of <0.05 was considered statistically significant.
Objectives: This study intended to assess the anxiolytic properties and safety of Fragaria ananassa fruit and leaves extracts in Swiss albino mice through the Open Field Test and acute toxicity evaluation.
Results: Different doses of Fragaria Ananassa fruit extract and leaves extract have shown statistically insignificant antianxiety effect. However clinically significant improvement on different anxiety parameters in mice is observed with different doses of fruit and leaves extracts. There was no toxicity or mortality observed during the acute toxicity study.
Conclusion: Using OFT, clinically significant improvement in anxiety is observed with strawberry fruit and leaves extracts and its LD50 is greater than 5000mg/kg.
References
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23. Hannum SM. Potential impact of strawberries on human health: a review of the science. Critical reviews in food science and nutrition. 2004 Jan 1;44(1):1-7.
24. Seeram NP, Lee R, Scheuller HS, Heber D. Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization mass spectroscopy. Food chemistry. 2006 Jul 1;97(1):1-1.
25. Gutierres JM, Carvalho FB, Schetinger MR, Marisco P, Agostinho P, Rodrigues M, Rubin MA, Schmatz R, da Silva CR, Cognato GD, Farias JG. Anthocyanins restore behavioral and biochemical changes caused by streptozotocin-induced sporadic dementia of Alzheimer's type. Life sciences. 2014 Feb 6;96(1-2):7-17.
26. Ibrahim DS, Abd El‐Maksoud MA. Effect of strawberry (Fragaria× ananassa) leaf extract on diabetic nephropathy in rats. International Journal of Experimental Pathology. 2015 Apr;96(2):87-93.
2. Whiteford HA, Degenhardt L, Rehm J, Baxter AJ, Ferrari AJ, Erskine HE, Charlson FJ, Norman RE, Flaxman AD, Johns N, Burstein R. Global burden of disease attributable to mental and substance use disorders: findings from the Global Burden of Disease Study 2010. The lancet. 2013 Nov 9;382(9904):1575-86.
3. Depression WH. Other common mental disorders: global health estimates. Geneva: World Health Organization. 2017 Feb;24(1).
4. Lakhan SE, Vieira KF. Nutritional and herbal supplements for anxiety and anxiety-related disorders: systematic review. Nutrition journal. 2010 Dec;9:1-4.
5. Carlini EA. Plants and the central nervous system. Pharmacology Biochemistry and Behavior. 2003 Jun 1;75(3):501-12.
6. Ravindran LN, Stein MB. The pharmacologic treatment of anxiety disorders: a review of progress. The Journal of clinical psychiatry. 2010 Jun 11;71(7):14308.
7. Hancock J, Simpson D. Methods of extending the strawberry season in Europe. HortTechnology. 1995 Oct 1;5(4):286-90.
8. Heide OM, Stavang JA, Sønsteby A. Physiology and genetics of flowering in cultivated and wild strawberries–a review. The Journal of Horticultural Science and Biotechnology. 2013 Jan 1;88(1):1-8.
9. Giampieri F, Forbes-Hernandez TY, Gasparrini M, Alvarez-Suarez JM, Afrin S, Bompadre S, Quiles JL, Mezzetti B, Battino M. Strawberry as a health promoter: an evidence based review. Food & function. 2015;6(5):1386-98.
10. Gould TD, Dao DT, Kovacsics CE. The open field test. Mood and anxiety related phenotypes in mice: Characterization using behavioral tests. 2009:1-20.
11. Hall CS. Drive and emotionality: factors associated with adjustment in the rat. Journal of Comparative Psychology. 1934 Feb;17(1):89.
12. Ivinskis A. A study of validity of open-field measures. Australian Journal of Psychology. 1970 Aug 1;22(2):175-83.
13. Cunny H, Hodgson E. Toxicity testing. A textbook of modern toxicology. 2004 Jan 30:351-97.
14. Machado DG, Bettio LE, Cunha MP, Santos AR, Pizzolatti MG, Brighente IM, Rodrigues AL. Antidepressant-like effect of rutin isolated from the ethanolic extract from Schinus molle L. in mice: evidence for the involvement of the serotonergic and noradrenergic systems. European Journal of Pharmacology. 2008 Jun 10;587(1-3):163-8.
15. Lorke D. A new approach to practical acute toxicity testing. Archives of toxicology. 1983 Dec;54:275-87.
16. Gupta V, Bansal P, Kumar S, Sannd R, Rao MM. Therapeutic efficacy of Phytochemicals as anti-anxiety-a review. J Pharm Res. 2010 Jan;3(1):174-9.
17. Rouillon F. Anxiety with depression: a treatment need. European Neuropsychopharmacology. 1999 Jul 1;9:S87-92.
18. Viola R, Brennan RM, Davies HV, Sommerville L. L-ascorbic acid accumulation in berries of Ribes nigrum L. The Journal of Horticultural Science and Biotechnology. 2000 Jan 1;75(4):409-12.
19. Valcheva-Kuzmanova S, Zhelyazkova-Savova M. Anxiolytic-like effect of Aronia melanocarpa fruit juice in rats. Methods Find Exp Clin Pharmacol. 2009 Dec 1;31(10):651-4.
20. Afridi GS, Ishaq M, Ahmad S. Cost and revenue analysis of strawberry production in the sub-tropical areas of NWFP, Pakistan. Pak. j. life soc. sci. 2009 Oct 7;7(1):59-65.
21. Salas-Arias K, Irías-Mata A, Sánchez-Kopper A, Hernández-Moncada R, Salas-Morgan B, Villalta-Romero F, Calvo-Castro LA. Strawberry Fragaria x ananassa cv. Festival: A polyphenol-based phytochemical characterization in fruit and leaf extracts. Molecules. 2023 Feb 16;28(4):1865.
22. Stewart AM, Gaikwad S, Kyzar E, Kalueff AV. Understanding spatio-temporal strategies of adult zebrafish exploration in the open field test. Brain research. 2012 Apr 27;1451:44-52.
23. Hannum SM. Potential impact of strawberries on human health: a review of the science. Critical reviews in food science and nutrition. 2004 Jan 1;44(1):1-7.
24. Seeram NP, Lee R, Scheuller HS, Heber D. Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization mass spectroscopy. Food chemistry. 2006 Jul 1;97(1):1-1.
25. Gutierres JM, Carvalho FB, Schetinger MR, Marisco P, Agostinho P, Rodrigues M, Rubin MA, Schmatz R, da Silva CR, Cognato GD, Farias JG. Anthocyanins restore behavioral and biochemical changes caused by streptozotocin-induced sporadic dementia of Alzheimer's type. Life sciences. 2014 Feb 6;96(1-2):7-17.
26. Ibrahim DS, Abd El‐Maksoud MA. Effect of strawberry (Fragaria× ananassa) leaf extract on diabetic nephropathy in rats. International Journal of Experimental Pathology. 2015 Apr;96(2):87-93.
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Apr 20, 2024
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How to Cite
Riffat Farooqui, Syeda Afroz, Hafiza Touseef Sayyar, Syeda Hafiza Afsheen Zafar, Fatima Rizvi, & Waqas Ahmed Farooqui. (2024). ANTIANXIETY POTENTIAL AND ACUTE TOXICITY ASSESSMENT OF FRAGARIA ANANASSA FRUIT AND LEAVES EXTRACT. Journal of Population Therapeutics and Clinical Pharmacology, 31(4), 2321-2330. https://doi.org/10.53555/6f7fwn08
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Author Biographies
Riffat Farooqui
Department of Pharmacology Dr. Ishrtul Ibad Institute of Oral Health Sciences. Dow University of Health Sciences. Karachi.
Syeda Afroz
Department of Pharmacology. Faculty of Pharmacy and Pharmaceutical Sciences. University of Karachi
Hafiza Touseef Sayyar
Department of Pharmacology. Bahria University Health Sciences. Campus Karachi.
Syeda Hafiza Afsheen Zafar
Department of Pharmacology. Bahria University Health Sciences. Campus Karachi.
Fatima Rizvi
Department of Pharmacology DIMC. Dow University of Health Sciences. Karachi.
Waqas Ahmed Farooqui
School of Public Health. Dow University of Health Sciences. Karachi
How to Cite
Riffat Farooqui, Syeda Afroz, Hafiza Touseef Sayyar, Syeda Hafiza Afsheen Zafar, Fatima Rizvi, & Waqas Ahmed Farooqui. (2024). ANTIANXIETY POTENTIAL AND ACUTE TOXICITY ASSESSMENT OF FRAGARIA ANANASSA FRUIT AND LEAVES EXTRACT. Journal of Population Therapeutics and Clinical Pharmacology, 31(4), 2321-2330. https://doi.org/10.53555/6f7fwn08