A NOVEL COMPARATIVE STUDY ON EFFICACY OF OZONATED OIL VERSUS SALINE ON INGLUVIES WOUND HEALING IN QUAILS (COTURNIX JAPONICA)

Abstract

Crop injuries are common in birds due to trauma, impaction or idiopathic in nature. There are many techniques to repair these injuries and to enhance the healing process. Ozone was discovered over a hundred years ago and since then it has been widely used in many areas.  Studies has revealed that ozone therapy i.e. ozonated saline and ozonated oil have revealed good healing of injured tissue. Moreover, use of ozonated preparations instead of antibiotics for healing purposes help to reduce the chances of antimicrobial resistance. In our studies, a total of 18 quails were selected and allocated to 03 groups (n=06). Ingluviotomy procedure was performed done on all the birds. After closure of incision, group B and C were treated with ozonated saline and ozonated oil respectively, while group A was administered normal saline (PO) @ 0.5ml per quail, three times a week for 20 days. This study was carried out for approximately 3 weeks and therapeutic regimens were monitored on day 0, 1, 5, 10, 15 and  20 in terms of physiological parameters, hematology indexes, leakage evaluation, histological evaluation and scanning electron microscopy. At the end of trials, we noticed excellent wound healing in group C (ozonated oil) due to more retention time with superior cell proliferation and fiber organization. Healing in group B (ozonated saline) was noted good due but it lagged group C in mentioned parameters, whereas group A (control) was found inferior than group B and C. These results highlighted the therapeutic potential of ozonated preparations in avian ingluvies wound management, so we may suggest that ozonated oil is marvelous in such cure while ozonated saline is note at 2^nd tier. However, further trials are needed for better understanding and routine clinical use of these regimens for avian practitioners.