Main Article Content
biofilm, enzyme, bacteria, EPS, nosocomial infections
Biofilms are complex communities of microorganisms encased within a matrix of extracellular polymeric substance (EPS) that they produce. These biofilms consist of microbial cells adhering to each other and to surfaces, whether living or non-living. Bacterial biofilms, in particular, are often associated with pathogenicity and can lead to nosocomial infections. In this study, we collected twenty samples from the industrial state of Hayatabad and employed pure culturing techniques along with biochemical tests to isolate and identify various bacterial species. The identified species included E. coli, S. aureus, Salmonella, Shigella, P. aeruginosa, Yersinia enterocolitis, Providencia, P. mirabilis, and B. subtilis. For our investigation into biofilm formation and degradation, we chose E. coli as the target bacterium and extracted enzymes from S. aureus. To assess biofilm formation, we utilized the microtiter plate assay. The optical density (OD) measurements for the control group were 1.749, and for replicate 1, they were 1.698 when treated with 20 μL of protease enzymes. For replicate 2, treated with 30 μL of enzymes, the OD was 1.582, and for replicate 3, treated with 50 μL of enzymes, the OD was 0.89. Our findings suggest that enzymatic treatment is a promising method for degrading biofilms, as evidenced by the substantial reduction in OD measurements. This research paves the way for further studies on a larger scale, focusing on the isolation and degradation of biofilms using enzymes. Understanding the potential of enzymatic degradation could lead to innovative strategies for combating biofilm-related infections.
2. Hancock, V., Dahl, M., & Klemm, P. (2010). Probiotic Escherichia coli strain Nissle 1917 outcompetes intestinalpathogens during biofilm formation. Journal of medical microbiology, 59(4), 392-399.
3. Hill, C., Guarner, F., Reid, G., Gibson, G. R., Merenstein, D. J., Pot, B., ... & Calder, P. C. (2014). Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature reviews Gastroenterology & hepatology, 11(8), 506.
4. Hong, S. H., Hegde, M., Kim, J., Wang, X., Jayaraman, A., & Wood, T. K. (2012). Synthetic quorum-sensing circuit to control consortial biofilm formation and dispersal in a microfluidic device. Nature communications, 3(1), 1-8.
5. Hyland, N. P., Quigley, E. M., & Brint, E. (2014). Microbiota-host interactions in irritable bowel syndrome: epithelial barrier, immune regulation and brain-gut interactions. World journal of gastroenterology: WJG, 20(27), 8859.
6. Jamal, M., Ahmad, W., Andleeb, S., Jalil, F., Imran, M., Nawaz, M. A., ... & Kamil, M. A. (2018). Bacterial biofilm and associated infections. Journal of the Chinese Medical Association, 81(1), 7-11.
7. Kay, M. K., Erwin, T. C., McLean, R. J., & Aron, G. M. (2011). Bacteriophage ecology in Escherichia coli and Pseudomonas aeruginosa mixed-biofilm communities. Applied and Environmental Microbiology, 77(3), 821-829.
8. Keogh, D., Tay, W., Ho, Y., Dale, J., Chen, S., Umashankar, S., ... & Kline, K. (2016). Enterococcal metabolite cues facilitate interspecies niche modulation and polymicrobial infection. Cell host & microbe, 20(4), 493-503.
9. Letoffe, S., Chalabaev, S., Dugay, J., Stressmann, F., Audrain, B., Portais, J. C., ... & Ghigo, J. M. (2017). Biofilm microenvironment induces a widespread adaptive amino-acid fermentation pathway conferring strong fitness advantage in Escherichia coli. PLoS genetics, 13(5), e1006800.
10. Lim, J. Y., Yoon, J. W., & Hovde, C. J. (2010). A brief overview of Escherichia coli O157: H7 and its plasmid O157. Journal of microbiology and biotechnology, 20(1), 5.
11. McBain, A. J. (2009). In vitro biofilm models: an overview. Advances in applied microbiology, 69, 99-132.
12. Oloketuyi, S. F., & Khan, F. (2017). Strategies for biofilm inhibition and virulence attenuation of foodborne pathogen-Escherichia coli O157: H7. Current microbiology, 74(12), 1477-1489
13. O'Toole, G. A. (2011). Microtiter dish biofilm formation assay. JoVE (Journal of Visualized Experiments), (47), e2437.
14. Rendueles, O., & Ghigo, J. M. (2012). Multi-species biofilms: how to avoid unfriendly neighbors. FEMS microbiology reviews, 36(5), 972-989.
15. Stressmann, F. A., Couve-Deacon, E., Chainier, D., Chauhan, A., Wessel, A., Durand-Fontanier, S., ... & Beloin, C. (2017). Comparative analysis of bacterial community composition and structure in clinically symptomatic and asymptomatic central venous catheters. Msphere, 2(5).
16. Vasudevan, R. (2014). Biofilms: microbial cities of scientific significance. J Microbiol Exp, 1(3), 00014.