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Fahad Said Khan
Muhammad Akram
Abid Rashid
Sultan Ayaz Sultan Ayaz
Muhammad Ali Shah


Elaeagnus parvifolia, hepatoprotective, renoprotective, antioxidant


The goal of this study was to see how albino mice responded to a hydroethanolic extract of Elaeagnus parvifolia fruits. Phytochemical analysis, FTIR spectroscopy and HPLC, findings confirmed the existence of functional groups and a wide spectrum of phenolic compounds, respectively. Furthermore, extract did not cause significant hemolysis (5.39±0.176%) when compared to PBS (0.103±0.15%) as a negative control; however, it did have significant clot lysis (21.20 ± 2.25%) potential, as well as antioxidant DPPH scavenging (37.023±0.204%), H2O2 scavenging (11.27±0.18%) and reducing power (81.47±0.24%). In vivo experiments in albino mice demonstrated that for 28 days giving extract dosages orally, after CCl4 intoxication significantly (P<0.05) recovered liver enzymes, renal profiles, lipid profile, serum electrolytes and stress markers, additionally the dosage group (200 mg/Kg b.w.) substantially (P<0.05) improved the structural architecture of liver tissue. Elaeagnus parvifolia has a substantial therapeutic response to treat medical difficulties, notably liver disorders, according to the findings.

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Ahsan R., Islam KM., Balbul IJ., Musaddik MA., and Haque E. Hepatoprotective Activity of Methanol Extract of Some Medicinal Plants Against Carbon Tetrachloride-Induced Hepatotoxicity in Rats. European Journal of Scientific Research. 2009, 37(2): 302-310.
Amjad, M. S., Arshad, M., & Qureshi, R. (2015). Ethnobotanical inventory and folk uses of indigenous plants from Pir Nasoora National Park, Azad Jammu and Kashmir. Asian Pacific Journal of Tropical Biomedicine, 5(3), 234-241.
Banu, K. S., & Cathrine, L. (2015). General techniques involved in phytochemical analysis. International Journal of Advanced Research in Chemical Science, 2(4), 25-32.
Bergmeyer HU, Horder M, Rej R. IFCC methods for the measurement of catalytic concentration of enzymes. Part 2. IFCC method for aspartate and alanine aminotransferases. J Clin Chem Clin Biochem. 1986;24:481-495.
Conti, C. R. (2002). Evolution of NCEP guidelines: ATP1‐ATPIII risk estimation for coronary heart disease in 2002. Clinical Cardiology, 25(3), 89.
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005;4(7): 685-688.
Feijoo M., Túnez I., Ruiz A., Tasset I., Muñoz E., and Collantes E. Oxidative stress biomarkers as indicator of chronic inflammatory joint diseases stage. Reumatología Clínica (English Edition). 2010, 6(2): 91-94.
Friedewald, W. T., Levy, R. I., & Fredrickson, D. S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry, 18(6), 499-502.
Ghiselli A, Serafini M, Natella F, Scaccin C. Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radical Biol Med. 2000;29(11):1106-1114.
Jacob JM., Olaleye TM., and Olugbuyiro OAJ. Hepatoprotective effect of Alchornea Cordifolia leaves on liver damage in albino rats, International Journal of Applied Science and Biotechnology. 2014, 2(2): 217-221.
Jain, P., Singh, S. K., Sharma, H. P., & Basri, F. (2014). Phytochemical screening and antifungal activity of Semecarpus anacardium L.(an anti-cancer plant). Int J Pharmaceut Sci Res, 5(5), 1884-1891.
Khan, R. A., Khan, M. R., Sahreen, S., & Bokhari, J. (2010). Prevention of CCl4-induced nephrotoxicity with Sonchus asper in rat. Food and Chemical Toxicology, 48(8-9), 2469-2476.
Koller A, Kaplan LA, Pesce AJ. (1984). Total Serum Protein, Clinical Chemistry, Theory, Analysis and Correlation. Mosby Company; 1984:1316-1319.
Kumar A., Rai N., Kumar N., Gautam P., and Kumar JS. Mechanisms involved in hepatoprotection of different herbal products, a review. Int J Res Pharm Sci. 2013, 4 (2):112-7.
Leake JR. Is diversity of ectomycorrhizal fungi important for ecosystem function?. New Phytol. 2001;152(1):1-3.
Liao RC., Chang SY., Peg HW., Lai CH., and Ho LY. Analgesic and anti-inflammatory activities of methanolic extract of elaeagnus oldhamii maxim in mice. American Journal of Chinese Medicine.2012, 40 (3): 581-597.
Naz, S., Hanif, M. A., Bhatti, H. N., & Shahid, M. (2016). Partition, fractionation, antioxidant potential and phenolics profiling of Cannabis sativa growing in Pakistan. Oxid. Commun, 39, 2946-2960.
Nweze CC, Mustapha AA, Alkali IM. Aqueous leaf extracts of Tobacco plant (Nicotiana tabaccum) causes hepatotoxicity in male Wistar albino rats. Asian J Pharmacol Toxicol. 2015;3(07):27-30.
Pandey, A. K., Verma, G., Vig, S., Srivastava, S., Srivastava, A. K., & Datta, M. (2011). miR-29a levels are elevated in the db/db mice liver and its overexpression leads to attenuation of insulin action on PEPCK gene expression in HepG2 cells. Molecular and cellular endocrinology, 332(1-2), 125-133.
Raghu, R., Liu, C. T., Tsai, M. H., Tang, X., Kalari, K. R., Subramanian, S., & Sheen, L. Y. (2012). Transcriptome analysis of garlic-induced hepatoprotection against alcoholic fatty liver. Journal of agricultural and food chemistry, 60(44), 11104-11119.
Rahman, M. A., Sultana, R., Bin Emran, T., Islam, M. S., Rahman, M. A., Chakma, J. S., ... & Hasan, C. M. M. (2013). Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity. BMC complementary and alternative medicine, 13(1), 1-7.
Rehman, F., S, Adeel, M. Shahid, I. A. Bhatti, F. Nasir, N. Akhtar, and Z. Ahmad. 2013. Dyeing of γ-irradiated cotton with natural flavonoid dye extracted from irradiated onion shells (Allium cepa) powder. Radiation Physics and Chemistry. 92: 71-75.
Riaz M, Mahmood Z, Saeed MUQ, Abbas M, Shahid M. Lipid peroxidation and antioxidant enzymes in relation to semen quality. Oxid Commun. 2016;39(4-I): 2990-2998.
Rubab, K.,Muhammad Athar Abbasi, Aziz-ur-Rehman, Sabahat Zahra Siddiqui, Syed Adnan Ali Shah, Muhammad Ashraf, Qurat-ul-Ain, Irshad Ahmad, Muhammad Arif Lodhi, Mehreen Ghufran, Muhammad Shahid and Hina Fatima. 2017. Synthesis, pharmacological screening and computational analysis of some 2-(1H-Indol-3-yl)-N’-[(un)substituted phenyl methylidene] acetohydrazides and 2-(1H-Indol-3-yl)-N’-[(un)substituted benzoyl/2- thienyl carbonyl] acetohydrazides. Pak. J. Pharm. Sci. 30(4):1263-1274.
Ruch, R. J., Cheng, S. J., & Klaunig, J. E. (1989). Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10(6), 1003-1008.
Saleem, A., Saleem, M., Akhtar, M. F., Shahzad, M., & Jahan, S. (2020). Moringa rivae leaf extracts attenuate Complete Freund’s adjuvant-induced arthritis in Wistar rats via modulation of inflammatory and oxidative stress biomarkers. Inflammopharmacology, 28(1), 139-151.
Shahid, C. S. A., A. I. Hussain, R. Asad, M. Majeed and N. Aslam. 2014. Bioactive components and antioxidant properties of Terminalia arjuna L. Journal of Food Processing & Technology. 5: 294-298.
Shahzadi, T., M. Zaib, T. Riaz, S. Shehzadi, M. A. Abbasi, and M. Shahid. 2019. "Synthesis of Eco-friendly Cobalt Nanoparticles Using Celosia argentea Plant Extract and Their Efficacy Studies as Antioxidant, Antibacterial, Hemolytic and Catalytical Agent." Arabian Journal for Science and Engineering: 1-10.
Sharif, S., Rashid, S., Atta, A., Irshad, A., Riaz, M., Shahid, M., & Mustafa, G. (2018). Phenolics, tocopherols and fatty acid profiling of wild and commercial mushrooms from Pakistan. Journal of biological regulators and homeostatic agents, 32(4), 863-867.
Shephard, M. D., & Whiting, M. J. (1990). Falsely low estimation of triglycerides in lipemic plasma by the enzymatic triglyceride method with modified Trinder's chromogen. Clinical chemistry, 36(2), 325-329.
Siedel, J., Hägele, E. O., Ziegenhorn, J., & Wahlefeld, A. W. (1983). Reagent for the enzymatic determination of serum total cholesterol with improved lipolytic efficiency. Clinical chemistry, 29(6), 1075-1080.
Singh, S., & Prasad, S. M. (2014). Growth, photosynthesis and oxidative responses of Solanum melongena L. seedlings to cadmium stress: mechanism of toxicity amelioration by kinetin. Scientia Horticulturae, 176, 1-10.
Thomas L. Clinical Laboratory Diagnostics. 1st ed. TH-Books Verlagsgesellschaft. 1998;36-46.
Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1): 44-84.
Walker, N., Gupta, R., & Cheesbrough, J. (2006). Blood pressure cuffs: friend or foe?. Journal of Hospital Infection, 63(2), 167-169.
Yadav, M., Yadav, A., & Yadav, J. P. (2014). In vitro antioxidant activity and total phenolic content of endophytic fungi isolated from Eugenia jambolana Lam. Asian Pacific journal of tropical medicine, 7, S256-S261.
Yue, Z. X., Gu, Y. X., Yan, T. C., Liu, F. M., Cao, J., & Ye, L. H. (2022). Phase Ⅰ and phase Ⅱ metabolic studies of Citrus flavonoids based on electrochemical simulation and in vitro methods by EC-Q-TOF/MS and HPLC-Q-TOF/MS. Food Chemistry, 132202.
Zheng X, Liu B, Li L, Zhu X. Micro-wave-assisted extraction and antioxidant activity of total phenolic compounds from pomegranate peel. J Med Plants Res. 2011;5(6):1004-1011.
Chatterjea, M. N., & Shinde, R. (2011). Textbook of medical biochemistry. Wife Goes On.
Prasad, S., Kashyap, R. S., Deopujari, J. Y., Purohit, H. J., Taori, G. M., & Daginawala, H. F. (2006). Development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thrombosis Journal, 4(1), 1-4.