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Rabia Syed
Najeeb Ullah
Muhammad Ikram
Saima Mumtaz Khattak
Sarwat Jahan


Parkinson’s disease, Lipopolysaccharide, Ethyl pyruvate, eurodegeneration, glial cells, inflammation, Substantia Nigra, motor disorder


Objective: This study aims to evaluate the therapeutic effects of Ethyl Pyruvate (EP) in lipopolysaccharide (LPS)-induced mouse model of Parkinson’s disease (PD).

 Methods: Total 24 male Balb-c mice were used for the study. 4 day repeat injection model of LPS (1mg/kg/day) was chosen. EP was administered half hour before the LPS in co-treatment group (40mg/kg/day). ELISA test was performed for serum analysis of TNF-alpha levels. Immunohistochemistry was done to analyze the Tyrosine Hydroxylase (TH) positive cell count in Substantia Nigra (SN) of mice brain. Pole behavioral test was done by recording Ttotal in seconds.

 Results: Our study showed that TH+ cell count was significantly reduced in LPS treated group as compared to control group. This count was improved after the administration of EP in co-treatment group. These effects of EP were supported by the results of ELISA, which showed significant reduction in TNF-alpha levels of co-treatment group (LPS + EP) in contrast to LPS treated group. Motor deficits were also improved as assessed by pole behavior test showing a reduction in Ttotal of (LPS + EP) group as compared to Ttotal of LPS treated group.

Conclusion: In this study we found significant improvement in dopaminergic neuron count, and motor deficits of PD model. Level of Inflammatory marker was also reduced significantly confirming the ant-inflammatory potential of EP. Together these data supports that progressive neurodegeneration of PD is slowed down after the use of EP.

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