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Noman Ullah Wazir
Zilli Huma
Irum Javaid
Farooq Khan
Amir Zaman Khan
Rabia Syed


Pirfenidone, Aneurysm Clip Model, Spinal Cord Compression Injury, neuronal degeneration, MAP2.


Aim: To explore the neuroprotective effect of pirfenidone in rat’s aneurysm clip compression spinal cord injury.

Methodology: A total of 30 healthy Sprague Dawley rats were randomly divided into spinal cord injury groups A, B and C. Group A received a placebo (n = 10), group B received 200 mg/kg/day of pirfenidone (n = 10) and group C received 500 mg/kg/day of pirfenidone. Based on the experimental duration of 14 and 28 days, each group was subdivided into groups 1 & 2 (n = 5 in each subgroup). An aneurysm clip with 70 g closing force was applied to the T7 level of the spinal cord for 1 minute to induce compression spinal cord injury. Immunohistochemistry by MAP2 anti-body for estimating viable neurons was performed.

Results: There were no normal viable neuronal cell bodies in the spinal cord injured areas in any groups. Nevertheless, neuronal cell body residues were detected in the injury sites and a statistically significant difference was witnessed within groups and between groups. The higher dose of 500 mg/kg/day of pirfenidone for 14 days slows down the process of neuronal degeneration in injury lesions compared to 200 mg/kg/day for a prolonged duration of 28 days.

Conclusion: Pirfenidone has no protective effect on neurons after spinal cord injury but due to its anti-oxidant and anti-inflammatory properties, it alters and delays the neurodegenerative process. This leads us to the future experimentation of pirfenidone in neurodegenerative diseases.

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