NEURONAL DEGENERATION CAN BE DECELERATED BY PIRFENIDONE AFTER COMPRESSION SPINAL CORD INJURY

Main Article Content

Noman Ullah Wazir
Zilli Huma
Irum Javaid
Farooq Khan
Amir Zaman Khan
Rabia Syed

Keywords

Pirfenidone, Aneurysm Clip Model, Spinal Cord Compression Injury, neuronal degeneration, MAP2.

Abstract

Aim: To explore the neuroprotective effect of pirfenidone in rat’s aneurysm clip compression spinal cord injury.


Methodology: A total of 30 healthy Sprague Dawley rats were randomly divided into spinal cord injury groups A, B and C. Group A received a placebo (n = 10), group B received 200 mg/kg/day of pirfenidone (n = 10) and group C received 500 mg/kg/day of pirfenidone. Based on the experimental duration of 14 and 28 days, each group was subdivided into groups 1 & 2 (n = 5 in each subgroup). An aneurysm clip with 70 g closing force was applied to the T7 level of the spinal cord for 1 minute to induce compression spinal cord injury. Immunohistochemistry by MAP2 anti-body for estimating viable neurons was performed.


Results: There were no normal viable neuronal cell bodies in the spinal cord injured areas in any groups. Nevertheless, neuronal cell body residues were detected in the injury sites and a statistically significant difference was witnessed within groups and between groups. The higher dose of 500 mg/kg/day of pirfenidone for 14 days slows down the process of neuronal degeneration in injury lesions compared to 200 mg/kg/day for a prolonged duration of 28 days.


Conclusion: Pirfenidone has no protective effect on neurons after spinal cord injury but due to its anti-oxidant and anti-inflammatory properties, it alters and delays the neurodegenerative process. This leads us to the future experimentation of pirfenidone in neurodegenerative diseases.

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