PREVALENCE OF METALLO BETA LACTAMASE PRODUCING PSEUDOMONAS AERUGINOSA & ANTIBIOTIC SENSITIVITY PATTERN IN A TERTIARY CARE HOSPITAL, NORTHERN INDIA
Main Article Content
Keywords
Pseudomonas aeruginosa, Metallo-Beta-Lactamase, Gram-negative bacteria, Imipenem,
Abstract
Introduction: Pseudomonas aeruginosa is the most frequent Gram-Negative bacteria detected in nosocomial infections and is an increasingly widespread opportunistic human disease. Pseudomonas aeruginosa is the causative agent of many different diseases, such as gastrointestinal tract infections, respiratory tract infections, skin irritation, soft tissue infections, bacteremia, skeletal and joint infections, and psoriasis.
Methods: In this study from July 2023 to July 2024. A total number of 156 cases of various clinical samples of Pseudomonas aeruginosa were isolated in Department of Microbiology, School of Health Sciences, C.S.J.M.U. Kanpur Sample were inoculated on Blood agar and MacConkey agar and Brain Heart Infusion Broth media were used for blood culture. The pseudomonas aeruginosa isolates were subjected to antibiotic susceptibility testing and Kirby-Bauer disc diffusion method for technique.
Results: A total number of 156 cases were, the study period from July 2023 to July 2024. 85 (54.48%) cases were find out positive ,out of them 49 (57.65%) positive cases were male and 36 (42.35%) positive cases were female This study further divided into different age group distribution , 10 - 20 age group obtained 8 (9.41%) positive cases and in age group of 51-60 is 9 (10.59%) cases turned out to be positive. In which the total number of MBL (metallo-beta-lactamase) positive cases are 28 (32.94%)
Conclusion: The study recommends incorporating MBL (metallo-beta-lactamase) detection methods into routine clinical laboratories due to the emergence and spread of MBL-producing pathogens. In the study results demonstrate the serious therapeutic and epidemiological threat of the spread of metallo-beta-lactamase producing Pseudomons aeruginosa. In this study, combined disc test (CDT) found to be best confirmatory tests as compared to double disc synergy test (DDST) in this part of country.
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Munish Rastogi
Department of Medical Microbiology, School of Health Sciences, C.S.J.M.U. Kanpur
Mohit Kumar
Department of Medical Microbiology, School of Health Sciences, C.S.J.M.U. Kanpur
Yashvi Gupta
Department of Medical Microbiology, School of Health Sciences, C.S.J.M.U. Kanpur
Anjali Verma
Department of Medical Microbiology, School of Health Sciences, C.S.J.M.U. Kanpur
Mansi Singh
Department of Medical Microbiology, School of Health Sciences, C.S.J.M.U. Kanpur
Tushar Sharma
Department of Medical Microbiology, School of Health Sciences, C.S.J.M.U. Kanpur
Dolly Rastogi
Professor & Head, Dept. of Physiology (G.S.V.M. Medical College Kanpur)