INTEGRATING COMPUTITONAL METHOD FOR DRUGGABLE BETA CATENIN INHIBITORY PHYTOCOMPOUND FROM GLYCINE MAX L. SEEDS TO TREAT OVARIAN CANCER

Main Article Content

Samra Hafeez
Asma Ahmed
Maryam Mureed
Hamna shahid
Afaq Akram

Keywords

Ovarian Cancer, Wnt/ß-catenin inhibition, Phytocompound, Glycine max L.

Abstract

Ovarian Cancer is the most lethal gynecological malignancy and is ranked asthe seventh leading cause of cancer deaths in females. Wnt signaling is an evolutionarily conserved regulatory pathway that governs numerous normal cellular and developmental processes such as cell fate determination, cell proliferation and migration. However, aberrant Wnt signaling has also been identified as a key mechanism in cancer biology. ß -catenin is the key mediator of the Canonical Wnt pathway. In the absence of a Wnt ligand, ß-catenin is degraded by a destruction complex. The main components of this complex include AXIN, adenomatous polyposis coli (APC), casein kinase 1 (CK1), and glycogen synthase kinase 3ß (GSK3ß), as well as the E3 ligase, ßTrCP. Protein phosphatase 2A (PP2A) is also associated with the ß-catenin destruction complex. AXIN is a scaffolding protein that has interaction sites for multiple proteins including PP2A, APC, GSK3ß, and CK1. The most common genetic alteration in the Wnt/ß-catenin pathway involved in EOC is in the ß-catenin gene, CTNNB1. In EOCs a missense mutation in CTNNB1 was always found within the amino terminal domain. Soybeans (Glycine max L.), a functional food widely consumed in Asia, has been reported as the main source of isoflavones. Phytoestrogen properties of soy isoflavones showed their activity as ligands for estrogen receptors and exhibited the estrogenic potency as reported in the previous in vitro and in vivo studies. The major functional components include Carbohydrates, fats, Proteins, Saponins, Lecithin, Linolenic acid, Linoleic acid, Phytosterols and Isoflavones. Due to these valuable constituents, it possesses multiple therapeutic activities. Current in-silico study showed that Daidzein from G. max L. has maximum binding affinity with beta catenin, which may be ultimately inhibited followed by the inactivation of Wnt/β -Catenin pathway. Moreover, it has been shown to relive sleep disorders, may help managing diabetes, prevents osteoporosis, improves blood circulation and provide good care of pregnancy.

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