AMELIORATIVE EFFECT OF QUERCETIN ON LIPID, ENZYMATIC, HORMONAL AND BIOCHEMICAL PROFILING OF STZ-INDUCED DIABETIC RATS.

Main Article Content

Farah Naz Tahir
Asma Ahmed
Gul-e-Rana
Khadija Kiran
Rehana Badar

Keywords

Camellia sinensis (L.), Quercetin, diabetic hyperlipidemia, hepato-renal and pancreatic protective, electrolytes, inflammatory markers

Abstract

Herbal medicine was commonly used before the development of other medicine with an increase in their scope of use each year. Camellia sinensis (L.) leaves important phyto-constituents like quercetin. In current study, therapeutic effects of intraperitoneally induced quercetin (10mg/mL/Kg b.w.) was checked to cure streptozotocin-induced (55mg/mL/Kg b.w.) diabetes in albino Wistar rats of both genders (300- 400 g), by keeping metformin (250mg/mL/Kg b.w.) as positive control, followed by the estimation of total lipids, cholesterol, triglycerides, HDL, LDL, VLDL, AST, ALT, ALP, , amylase, lipase, LDH ,urea, creatinine, uric acid, bilirubin, albumin, globulin, A/G ratio, total proteins, sodium, potassium, calcium, magnesium, chloride and HbA1clevels by kit methods. Statistically analyzed results (at p≤ 0.05) revealed that quercetin have improved the levels of cholesterol (88.25±10.47 and 47.3±3.0 mg/dL), triglycerides (46.00±5.72 and 50±8.0 mg/dL), HDL (57.50±1.29 and 26.3±2.0 mg/dL), LDL (29.75±6.95 and 16.3±1.0 mg/dL), VLDL (16.50±1.29 and 32.3±1.2 mg/dL), Urea (3.1±0.8 and 1.26±0.2 mg/dL), creatinine (0.7±0.1 and 1.26±0.2 mg/dL), uric acid (1.6±0.16 and 1,56±0.08 mg/dL), bilirubin (0.43±0.02 and 0.3±0.05 mg/dL), AST (77.14±7.0 and 59±4.5 U/L), ALT (31.2±4.8 and 34.3±2.0 U/L), ALP (85±2.5 and 56.6±2.5 U/L), albumin (2.3±0.9 and 3.9±0.08 mg/dL), globulin (3.82±0.4 and 2.82±0.4 mg/dL), A/G ratio (1.01±0.6 and 2.0±0.5 %), total proteins (4.9±0 and 3.3±0.03 mg/dL) and HbA1C (3.7±0.89 and 3.9±0,01 %) in both male and female rats respectively while sodium (138.14±6.76 mmol/L), potassium (4.5±0.33 mmol/L), calcium (9.9±1.72 mg/dL), magnesium (1.7±0.44 mmol/L), chloride (106.50±8.5 mmol/L), amylase (443.79±29.28 U/L), lipase (18.6±4.1 U/L), LDH (688.86±13.54 U/L) were same in both genders, as compared to negative control [cholesterol (95.25±10.47 and 105±7.0 mg/dL), triglycerides (87.00±5.72 and 82.3±2.0 mg/dL), HDL (57.00±5.36 and 64.3±4.0 mg/dL), LDL (28.75±5.56 and 25.3±1.0 mg/dL), VLDL (13.25±1.71 and 12.6±0.5 U/L), Urea (80±6.0 and 67.5±3.8 mg/dL), creatinine (1.31±0.1 and 0.8±0.02 mg/dL), uric acid (1.6±0.10 ad 1.8±0.1 mg/dL), bilirubin (2.06±0.3 and 1.26±0.05 mg/dL), AST (199.83±8.3 and 155.5±5 U/L), ALT (72.3±5.6 and 77.1±4.8 U/L), ALP (95±3 and 89±6.3 U/L), albumin (2.9±0.5 and 3.36±0.04 mg/dL), globulin (1.03±0.03 and 2.4±0.2 mg/dL), total protein (6.56±0.7 and 6.64±2 mg/dL), A/G ratio (1.1±0.1 % in both), sodium (144.14±8.76 and 132.6±12 mmol/L), potassium (7.25±3.38 and 5.9±0.03 mmol/L), calcium (9.25±1.7 and 10.5±4.0 mmol/L), magnesium (4.01±0.448 and 2.5±0.2 mmol/L), chloride (101.50±0.58 and 106±10 mmol/L), amylase (312.6±10.28 and 418±13 U/L), lipase (180± 9.2 and 29.4±4.0 U/L), LDH (1654±164 and 1771±98 U/L) and HbA1c (4.4±0.29 and 3.2±0.4%)] while effect of quercetin extract was almost similar to positive control drug or metformin. Histopathological analysis showed recovery of renal, hepatic, and pancreatic tissues, along with a reduction in mononuclear cell infiltrate and improvement in steatosis in the liver, while pancreas showed mild restoration of pancreatic beta cells. Current results can be used for the isolation and hepato-renal safety profiling of quercetin from C. sinesnsis (L.) via its bioactivity guided isolation.

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References

1. Asma A, Gulfraz M, Javaid AM, Rehmatullah Q, Shabnam B and Imam SS. 2016. The hypoglycemic and hypercholesterolemic activity of few medicinal plants against Streptozotocin induced hyperglycemia. Pakistan journal of pharmaceutical sciences. 29; (6):2065-2070.
2. Al-Awaida WJ, Zihlif MA, Al-Ameer HJ, Sharab A, Akash M, Aburubaiha ZA, Fattash IA, Imraish A, Ali KH .2019. The effect of green tea consumption on the expression of antioxidant- and inflammation-related genes induced by nicotine. Journal of food biochemistry.43; (7):12874.
3. Amit Kumar Singh, Harvesh Kumar Rana, Vishal Singh, Tara Chand Yadav, PritishVaradwaj, Abhay Kumar Pandey. (2021). Evaluation of the antidiabetic activity of dietary phenolic compound chlorogenic acid in streptozotocin-induced diabetic rats. Molecular docking, molecular dynamics, in silico toxicity, in vitro, and in vivo studies. Computers in Biology and Medicine, 134: 0010-4825.
4. Amalraj A, Gopi S. 2017. Medicinal properties of Terminaliaarjuna (Roxb.) Journal of Traditional Complementary Medicine.7.6: 765-778
5. Anouar Ben Saad, Sana Ncib, IlhemRjeibi, IssamSaidi, and Nacim Zouari.2017. Nephroprotective and antioxidant effect of green tea (Camellia sinensis) against nicotine-induced nephrotoxicity in rats and characterization of its bioactive compounds by HPLC–DAD. Applied Physiology, Nutrition, and Metabolism, 44(11): 1134-1140.
6. Amrani A, Boubekri N, Lassad S, Zama D, Benayache F, Benayache S.2017. Alcohol Induced HepatoCardiotoxicity and Oxidative Damage in Rats.The Protective Effect of n-butanol Extract of Green Tea Camellia sinensis. Cardiovascular HematologicalDisorders Drug Targets, 4.17(1):18-23.
7. Antinone, Richard RN, CEN, MSN; Kress, Terri RN, CEN, MSN. 2009.Measuring serum lactate. Nursing Critical Care, 4(5):56.
8. Bonuccelli G, Sotgia F, Lisanti MP.2018. Matcha green tea (MGT) inhibits the propagation of cancer stem cells (CSCs), by targeting mitochondrial metabolism, glycolysis and multiple cell signaling pathways. Aging (Albany NY), 10 (8):1867-1883
9. Bag A, Bag N.(2018).Tea Polyphenols and Prevention of Epigenetic Aberrations in Cancer.Journal of Natural Science, Biology and Medicine,9(1):2-5.
10. Bamanikar, S. A., Bamanikar, A. A., and Arora, A. 2016. Study of Serum urea and Creatinine in Diabetic and nondiabetic patients in a tertiary teaching hospital. The Journal of Medical Research, 2(1): 12-15.
11. Ullah, L., Hameed, Y., Ejaz, S., Raashid, A., Iqbal, J., Ullah, I. and Ejaz, S.A., 2020. Detection of novel infiltrating ductal carcinoma-associated BReast CAncer gene 2 mutations which alter the deoxyribonucleic acid-binding ability of BReast CAncer gene 2 protein. Journal of Cancer Research and Therapeutics, 16(6), pp.1402-1407.
12. Barrios V, Escobar C, Giuseppe Cicero AF, Burke D, Fasching P, Banach M, Bruckert E.2017. A nutraceutical approach (Armolipid Plus) to reduce total and LDL cholesterol in individuals with mild to moderate dyslipidemia.24:1–15.
13. Cardoso RR, Neto RO, Dos Santos D'Almeida CT, do Nascimento TP, Pressete CG, Azevedo L, Martino HSD, Cameron LC, Ferreira MSL, Barros FAR.2020.Kombuchas from green and black teas have different phenolic profile, which impacts their antioxidant capacities, antibacterial and antiproliferative activities Food Research International journal. 128:108782.
14. Ghimire R, Thapa AS, Karki D, Shrestha DK. Routine Measurement of Serum Amylase in Acute Abdomen. Journal of Nepal Medical Association. 52(196):982-5.
15. Huang, F., Zheng, X., Ma, X., Jiang, R., Zhou, W., Zhou, S., Zhang, Y., Lei, S., Wang, S., Kuang, J., Han, X., Wei, M., You, Y., Li, M., Li, Y., Liang, D., Liu, J., Chen, T., Yan, C., Wei, R. Jia, W. 2019. Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism. Nature communications, 10(1):4971.
16. Jiang Shi, Yin Zhu, Yue Zhang, Zhi Lin, Hai-Peng Lv. 2019.Volatile composition of Fu-brick tea and Pu-erh tea analyzed by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry. LWT-food science and technology, 103: 27-33.
17. Hirano T, Nishioka F, Murakami T. 2004. Measurement of the serum lipoprotein lipase concentration is useful for studying triglyceride metabolism Comparison with postheparin plasma. Metabolism. 53(4):526-31.
18. Hou Y, Ding T, Guan Z, Wang J, Yao R, Yu Z, Zhao X.2023. Untargeted metabolomics reveals the preventive effect of quercetin on nephrotoxicity induced by four organophosphorus pesticide mixtures.Food and Chemical Toxicology.175:113747.
19. Koch W, Kukula-Koch W, Komsta Ł, Marzec Z, Szwerc W, Głowniak K Molecules. 2018. Green Tea Quality Evaluation Based on Its Catechins and Metals Composition in Combination with Chemometric Analysis. Molecules, 23(7):1689.
20. Khaerunnisa, S., Kurniawan, H., Awaluddin, R., Suhartati, S., Soetjipto, S., 2020. Potential Inhibitor of COVID-19 Main Protease (Mpro) From Several Medicinal Plant Compounds by Molecular Docking Study. Preprints.
21. Kellogg JJ, Graf TN, Paine MF, McCune JS, Kvalheim OM, Oberlies NH, Cech NB.2017. Comparison of Metabolomics Approaches for Evaluating the Variability of Complex Botanical Preparations Green Tea Camellia sinensis as a Case Study. Journal of Natural Products, 80(5):1457-1466.
22. Li F, Wang Y, Li D, Chen Y, Qiao X, Fardous R, Lewandowski A, Liu J, Chan TH, Dou QP.2018. Perspectives on the recent developments with green tea polyphenols in drug discovery.Expert Opinion on Drug Discovery, 13(7):643-660.
23. Musial C, Kuban-Jankowska A, Gorska-Ponikowska M.2020. Beneficial Properties of Green Tea Catechins. International Journal of Molecular Sciences, 21(5):1744.
24. Maity R, Chatterjee M, Banerjee A, Das A, Mishra R, Mazumder S, Chanda N.2019. Gold nanoparticle-assisted enhancement in the anti-cancer properties of theaflavin against human ovarian cancer cells. Material Sciences and Engineering, 104:109909.
25. Mhatre, S., Srivastava, T., Naik, S., &Patravale, V. 2021. Antiviral activity of green tea and black tea polyphenols in prophylaxis and treatment of COVID-19. International journal of phytotherapy and phytopharmacology, 85: 153286.
26. Nirumand, M. C., Hajialyani, M., Rahimi, R., Farzaei, M. H., Zingue, S., Nabavi, S. M., &Bishayee, A. 2018. Dietary Plants for the Prevention and Management of Kidney Stones Preclinical and Clinical Evidence and Molecular Mechanisms. International journal of molecular sciences, 19(3): 765.
27. Yasir, M., Nawaz, A., Ghazanfar, S., Okla, M.K., Chaudhary, A., Al, W.H., Ajmal, M.N., AbdElgawad, H., Ahmad, Z., Abbas, F. and Wadood, A., 2022. Anti-bacterial activity of essential oils against multidrug-resistant foodborne pathogens isolated from raw milk. Brazilian Journal of Biology, 84, p.e259449.
28. Sial, N., Saeed, S., Ahmad, M., Hameed, Y., Rehman, A., Abbas, M., Asif, R., Ahmed, H., Hussain, M.S., Rehman, J.U. and Atif, M., 2021. Multi-omics analysis identified TMED2 as a shared potential biomarker in six subtypes of human cancer. International Journal of General Medicine, pp.7025-7042.
29. Prasanth, M. I., Sivamaruthi, B. S., Chaiyasut, C., &Tencomnao, T. 2019. A Review of the Role of Green Tea Camellia sinensis in Antiphotoaging, Stress Resistance, Neuroprotection, and Autophagy. Nutrients, 11(2):474.
30. Pezeshki, A., Safi, S., Feizi, A., Askari, G., &Karami, F. 2016. The effect of green tea extract supplementation on liver enzymes in patients with nonalcoholic fatty liver disease. International Journal of Preventive Medicine, 7:28.
31. Zhang, L., Sahar, A.M., Li, C., Chaudhary, A., Yousaf, I., Saeedah, M.A., Mubarak, A., Haris, M., Nawaz, M., Reem, M.A. and Ramadan, F.A., 2022. A detailed multi-omics analysis of GNB2 gene in human cancers. Brazilian Journal of Biology, 84.
32. Shams-Ghahfarokhi M, Shokoohamiri MR, Amirrajab N, Moghadasi B, Ghajari A, Zeini F, et al. 2006. In vitro antifungal activities of Allium cepa, Allium sativum and ketoconazole against some pathogenic yeasts and dermatophytes. Fitoterapia.77:321–323.
33. Mao, J., Huang, X., Okla, M.K., Abdel-Maksoud, M.A., Mubarak, A., Hameed, Z., Noreen, R., Chaudhary, A., Ghazanfar, S., Liao, Y. and Hameed, Y., 2022. Risk Factors for TERT promoter mutations with papillary thyroid carcinoma patients: a meta-analysis and systematic review. Computational and Mathematical Methods in Medicine, 2022.
34. Sharifi-Rad M, Pezzani R, Redaelli M, Zorzan M, Imran M, Ahmed Khalil A, Salehi B, Sharopov F, Cho WC, Sharifi-Rad J.2020. Preclinical Pharmacological Activities of Epigallocatechin-3-gallate in Signaling Pathways.Molecules,25(3):467.
35. Tahir UlQamar M., Alqahtani S., Alamri M., Chen L.2020.Structural basis of SARS-CoV-2 3CLpro and anti-COVID-19 drug discovery from medicinal plants. Journal of Pharmaceutical Analysis, 10(4):313-319.
36. Ye Y., Yan J., Cui J., Mao S., Li M., Liao X.2018. Dynamic changes in amino acids, catechins, caffeine and gallic acid in green tea during withering. Journal of Food Composition and Analysis, 66:98–108.
37. Yakubu MT, Akanji MA, Oladiji AT .2007. Male sexual dysfunction and methods used in assessing medicinal plants with aphrodisiac potentials. Pharmacognosy. 1.48
38. Zheng, Y.; Ley, S.H.; Hu, F.B. 2018.Global etiology and epidemiology of type 2 diabetes mellitus and its complications. Nature reviews endocrinology, 14:88.
39. Amit Kumar Singh, Harvesh Kumar Rana, Vishal Singh, Tara Chand Yadav, PritishVaradwaj, Abhay Kumar Pandey. (2021). Evaluation of the antidiabetic activity of dietary phenolic compound chlorogenic acid in streptozotocin-induced diabetic rats. Molecular docking, molecular dynamics, in silico toxicity, in vitro, and in vivo studies. Computers in Biology and Medicine, 134: 0010-4825.
40. Anouar Ben Saad, Sana Ncib, IlhemRjeibi, IssamSaidi, and NacimZouari.(2017). Nephroprotective and antioxidant effect of green tea (Camellia sinensis) against nicotine-induced nephrotoxicity in rats and characterization of its bioactive compounds by HPLC–DAD. Applied Physiology, Nutrition, and Metabolism, 44(11): 1134-1140.
41. Ali, A. H. A. (2018). Hepatoprotective effect of green tea extract against cyclophosphamide induced liver injury in Albino rats. Forensic Medicine and Anatomy Research, 6(2): 11-19.
42. Draelos ZD (2008). The ability of onion extract gel to improve the cosmetic appearance of postsurgical scars. Journal of Cosmetic Dermatology, 7:101–104.
43. Franco RR, Ribeiro Zabisky LF, Pires de Lima Júnior J, MotaAlves VH, Justino AB, Saraiva AL, Goulart LR, Espindola FS.( 2020). Antidiabetic effects of Syzygiumcumini leaves A non-hemolytic plant with potential against process of oxidation, glycation, inflammation and digestive enzymes catalysis. Journal of Ethnopharmacol. 28(261):113-132.
44. Galavi A, Hosseinzadeh H, Razavi BM. (2021).The effects of Allium cepa L. (onion) and its active constituents on metabolic syndrome. Iranian Journal of Basic Medical Sciences, 24(1):3-16
45. Hill MF, Bordoni B. (2021). Hyperlipidemia. Stat Pearls
46. Kumar VP, Venkatesh YP.(2016). Alleviation of cyclophosphamide-induced immunosuppression in Wistar rats by onion lectin (Allium cepa agglutinin) Journal of Ethnopharmacol, 186:280–288
47. Khajah MA, Orabi KY, Hawai S, Sary HG, El-Hashim AZ. (2019). Onion bulb extract reduces colitis severity in mice via modulation of colonic inflammatory pathways and the apoptotic machinery. Journal of Ethnopharmacology,241:112008.
48. Kim JH.(1997). Antibacterial action of onion (Allium cepaL ) extracts against oral pathogenic bacteria. The Journal of Nihon University School of Dentistry, 39:136–141.
49. Lee SM, Moon J, Chung JH, Cha YJ, Shin MJ.(2013). Effect of quercetin-rich onion peel extracts on arterial thrombosis in rats. Food and Chemical Toxicology, 57:99–105.
50. Li F, Wang Y, Li D, Chen Y, Qiao X, Fardous R, Lewandowski A, Liu J, Chan TH, Dou QP.(2018). Perspectives on the recent developments with green tea polyphenols in drug discovery.Expert Opinion on Drug Discovery, 13(7):643-660.
51. Li, T., R.M. Horton, D.A. Bader, F. Liu, Q. Sun, and P.L. Kinney.(2018). Long-term projections of temperature-related mortality risks for ischemic stroke, hemorrhagic stroke, and acute ischemic heart disease under changing climate in Beijing, China. Environment International journal, 112: 1-9.
52. Mousum, S.A., Ahmed, S., Gawali(2018). Nyctanthes arbor-tristis leaf extract ameliorates hyperlipidemia- and hyperglycemia-associated nephrotoxicity by improving anti-oxidant and anti-inflammatory status in high-fat diet–streptozotocin-induced diabetic rats. Inflammopharmacology, 26(6):1415-1428.
53. Durack, J., Lynch, S. V., Nariya, S., Bhakta, N. R., Beigelman, A., Castro, M 2017. Features of the bronchial bacterial microbiome associated with atopy, asthma, and responsiveness to inhaled corticosteroid treatment. Journal of allergy and clinical immunology, 140(1), 63-75.
54. Peng Z, Gong X, Yang Y, Huang L, Zhang Q, Zhang P. (2017). Hepatoprotective effect of quercetin against LPS/d-GalN induced acute liver injury in mice by inhibiting the IKK/NF-kappaB and MAPK signal pathways. International Immuno Pharmacology, 52:281–289.
55. Ren KW, Li YH, Wu G, Ren JZ, Lu HB, Li ZM.(2017). Quercetin nanoparticles display antitumor activity via proliferation inhibition and apoptosis induction in liver cancer cells. The International Journal of Oncology, 50:1299–1311.
56. Ro JY, Ryu JH, Park HJ, Cho HJ.(2015). Onion (Allium cepa) peel extract has anti-platelet effects in rat platelets. Springerplus,13 (4):17.
57. Reddy RRL, Srinivasan K.(2011). Dietary fenugreek and onion attenuate cholesterol gallstone formation in lithogenic diet-fed miceInternational Journal of Experimental Pathology,92:308–319.
58. Singh T, Goel RK.(2015). Neuroprotective effect of Allium cepa L in aluminum chloride induced neurotoxicity. Neurotoxicology, 49:1–7.
59. Shams-Ghahfarokhi M, Shokoohamiri MR, Amirrajab N, Moghadasi B, Ghajari A, Zeini F, et al. (2006). In vitro antifungal activities of Allium cepa, Allium sativum and ketoconazole against some pathogenic yeasts and dermatophytes. Fitoterapia, 77:321–323.
60. Susilowati R, Jannah J, Maghfuroh Z, Kusuma MT.(2020). Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats. Journal of Advanced Pharmaceutical Technology & Research, 11(3):128-133.
61. Wang-Fischer, Y., and Garyantes, T. (2018). Improving the reliability and utility of streptozotocin-induced rat diabetic model. Journal of Diabetes Research, 1-14.
62. Hasanuzzaman, M., Bhuyan, M. B., Zulfiqar, F., Raza, A., Mohsin, S. M., Mahmud, J. A. & Fotopoulos, V. (2020). Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial role of a universal defense regulator. Antioxidants, 9(8), 681.
63. Zhang C, Suen CL, Yang C, Quek SY.(2017). Antioxidant capacity and major polyphenol composition of teas as affected by geographical location, plantation elevation and leaf grade,Food Chemistry, 1. 244:109-119.

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