PHYTOCOMPOUND-BASED DRUG DISCOVERY APPROACH TO EXPLORE THE FROSTBITE HEALING POTENTIAL OF ABIETADIENE ISOLATED FROM PINUS ROXBURGHII

Main Article Content

Hasan Akbar Khan
Asma Ahmed
Khadija Kiran
Samra Hafeez
Rehana Badar
Sajjad Ahmad

Keywords

Abstract

Exposure to subzero temperatures usually leads to vascular damage causing severe ischemic injury known as frostbite, one of the prominent cold weather injuries that can lead to devastating consequences such as amputation of the extremities. Although rate of amputation due to frostbite has decreased recently due to thrombolytic therapy but this new regimen comes with a price as well which include symptoms such as profuse gastrointestinal bleeding in patients. Thus, there is a need to discover effective treatments with least side effects and more bioavailability rate. In current work, aqueous extract of Pinus roxburghii leaves were checked against dry-ice induced frostbite on plantar surfaces of albino Wistar rats (both genders, 150-200 g) by keeping heparin as control, followed by the computational evaluation of its phytocompounds to prioritize potential antiinflammatory and anti-thrombotic compounds against frostbite. Statistically analyzed results of molecular docking showed that among all secondary metabolites of P.roxburghii, abietadiene was most suitable potential ligand against antiplasmin and antithrombin III, which modulated anticoagulant pathway and proved to be a valuable anti-inflammatory and antithrombotic agent for wound healing. These results suggest the wound healing potential of abietadiene especially in case of frost bite which further needs to be validated experimentally.

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