ANTIDIABETIC EFFECTS OF PROTEIN ENRICHED BARLEY BASED EXTRUDED SNACKS AGAINST STREPTOZOTOCIN INDUCED DIABETES IN SPRAGUE DAWLEY RATS

Main Article Content

Anam Hameed
Allah Rakha
Masood Sadiq Butt
Muhammad Asghar

Keywords

Diabetes, Barley, Sunflower seeds, Protein diet, Beta glucan, Biochemical tests, Histopathology

Abstract

Several studies revealed that protein and dietary fiber enriched diet has therapeutic effect against hyperglycemia or diabetes mellitus. Diabetes mellitus is one of the major non-communicable diseases and the leading cause of deaths in developing countries and around the globe. This study aimed to evaluate the antidiabetic effect of protein enriched barley based extruded snacks against the diabetic rats. For this purpose, barley based extruded snacks with the incorporation of 20% defatted sunflower meal were used for efficacy trials on Sprague Dawley rats. In this context, 40 healthy rats were selected and divided into four groups i.e., negative control (NC), positive control (PC), standard drug (SD) and treatment (EB20) groups. Negative control were fed on normal diet and hyperglycemia was induced by using 2 doses of Streptozotocin injection along with the administration of normal diet (PC). SD group receive metformin per kg body weight for 28 days after the induction of hyperglycemia. While treatment group received treatment diet for 28 days  as long as the hyperglycemic condition resulted. Blood glucose was recorded and monitored on regular basis. Blood samples were collected and analyzed for different hyperglycemic parameters, kidney and liver stress biomarkers. Hispathological examination of pancreatic tissues was also conducted. The results of biochemical tests and the examination of histopathological tissues of rats fed on protein enriched barley based diet revealed that the adminitation of protein diet significantly lowered the blood glucose or insulin levels does improve the serum glucose, serum insulin, HbA1c, T3, T4, and TSH levels and less likely to affect pancreas.

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