Developing Sciatic nerve compression model in rats using aneurysm clip and validating it by histological and behavior studies

Main Article Content

Nazish Waheed
Zilli Huma
Noman Ullah Wazir
Fatima Daud
Saima Mumtaz
Sara Ishaq

Keywords

Aneurysm Clip Compression, Sciatic Nerve Injury

Abstract

 Aim: The aim of the study was to develop sciatic nerve compression model in rats using aneurysm
clip And to validate compression injury by histological examination and behavior testing
Study design and duration: experimental animal model
Methodology: The study included 16 healthy Sprague Dawley rats weighing 250 to 300 gm. The rats
were divided into two groups. Each group comprised of 4 male and 4 female rats. Sham group animals
were given only skin incision while in control group rats’ sciatic nerve injury was induced in right
limb under isoflurane anesthesia with an aneurysm clip having a force of 0.6 N. Animals were
observed on day 0, 1 and 7 for behavior changes
Results: On histological and behavior examination the animals showed marked changes in crush
injury group. On microscopy of tissue after H & E staining clearly evident injury was seen with
discontinuity in architecture of nerve and macrophage infiltration at the site of application of
aneurysm clamp. Animals in Injury group showed marked mechanical and thermal hyperalgesia and
cold allodynia as compared to sham group.
Conclusion: The evident histological alterations supported by the behavior changes in the injury
group confirm that the aneurysm clip model is a valid, self-effacing and easily reproducible method
to induce sciatic nerve injury in rats with uniform force application for experimental studies.

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References

1. An, Y., Yan, H.-X., Zhao, J.-N., Yang, X.-M., & Yan, J.-T. (2022). Evaluation methods of a rat sciatic nerve crush injury model. Journal of Integrative Neuroscience, 21(3), 91.
2. Baliki, M., Calvo, O., Chialvo, D. R., & Apkarian, A. V. (2005). Spared nerve injury rats exhibit thermal hyperalgesia on an automated operant dynamic thermal escape task. Molecular pain, 1, 1744-8069-1741-1718.
3. Bonin, R. P., Bories, C., & De Koninck, Y. (2014). A simplified up-down method (SUDO) for measuring mechanical nociception in rodents using von Frey filaments. Molecular pain, 10(1), 1-11.
4. Bridge, P. M., Ball, D. J., Mackinnon, S. E., Nakao, Y., Brandt, K., Hunter, D. A., & Hertl, C. (1994). Nerve crush injuries—a model for axonotmesis. Experimental neurology, 127(2), 284-290.
5. Câmara, C. N. d. S., Brito, M. V. H., Silveira, E. L., Silva, D. S. G. d., Simões, V. R. F., & Pontes, R. W. F. (2011). Histological analysis of low-intensity laser therapy effects in peripheral nerve regeneration in Wistar rats. Acta cirurgica brasileira, 26, 12-18.
6. de Almeida Melo Maciel Mangueira, M., Caparelli-Dáquer, E., de Assis, D. S. F. R., Sousa, J. K. C., Lima, W. L., Pinheiro, A. L. B., . . . Mangueira, N. M. (2022). Raman spectroscopy and sciatic functional index (SFI) after low-level laser therapy (LLLT) in a rat sciatic nerve crush injury model. Lasers in Medical Science, 1-15.
7. DeLeonibus, A., Rezaei, M., Fahradyan, V., Silver, J., Rampazzo, A., & Bassiri Gharb, B. (2021). A meta‐analysis of functional outcomes in rat sciatic nerve injury models. Microsurgery, 41(3), 286-295.
8. Geuna, S. (2015). The sciatic nerve injury model in pre-clinical research. Journal of neuroscience methods, 243, 39-46.
9. Gurkan, G., Erdogan, M. A., Yigitturk, G., & Erbas, O. (2021). The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model. Journal of Korean Neurosurgical Society, 64(6), 873-881.
10. Mazzer, P. Y. C. N., Barbieri, C. H., Mazzer, N., & Fazan, V. P. S. (2008). Morphologic and morphometric evaluation of experimental acute crush injuries of the sciatic nerve of rats. Journal of neuroscience methods, 173(2), 249-258.
11. Omura, T., Sano, M., Omura, K., Hasegawa, T., & Nagano, A. (2004). A mild acute compression induces neurapraxia in rat sciatic nerve. International journal of neuroscience, 114(12), 1561-1572.
12. Rosen, S., Ham, B., & Mogil, J. S. (2017). Sex differences in neuroimmunity and pain. Journal of neuroscience research, 95(1-2), 500-508.
13. Sarikcioglu, L., & Ozkan, O. (2003). Instrumentation Note: YASARGIL-PHYNOX ANEURYSM CLIP: A SIMPLE AND RELIABLE DEVICE FOR MAKING A PERIPHERAL NERVE INJURY. International journal of neuroscience, 113(4), 455-464.
14. Sasidharan, G. M., Sastri, S. B., & Pandey, P. (2015). Aneurysm clips: What every resident should know. Neurology India, 63(1), 96.
15. Sasso, L. L., de Souza, L. G., Girasol, C. E., Marcolino, A. M., de Jesus Guirro, R. R., & Barbosa, R. I. (2020). Photobiomodulation in sciatic nerve crush injuries in rodents: a systematic review of the literature and perspectives for clinical treatment. Journal of Lasers in Medical Sciences, 11(3), 332.
16. Sharp, P., Tyreman, N., Jones, K., & Gordon, T. (2018). Crush injury to motor nerves in the G93A transgenic mouse model of amyotrophic lateral sclerosis promotes muscle reinnervation and survival of functionally intact nerve-muscle contacts. Neurobiology of disease, 113, 33-44.
17. Siwei, Q., Ma, N., Wang, W., Chen, S., Wu, Q., Li, Y., & Yang, Z. (2022). Construction and effect evaluation of different sciatic nerve injury models in rats. Translational Neuroscience, 13(1), 38-51.
18. Yoon, C., Wook, Y. Y., Sik, N. H., Ho, K. S., & Mo, C. J. (1994). Behavioral signs of ongoing pain and cold allodynia in a rat model of neuropathic pain. Pain, 59(3), 369-376.
19. Yucel, M., Aktas, O. Y., Zengi, O., Tas, A., Tufan, A., Eren, B., & Guzey, F. K. (2023). The effect of alpha-lipoic acid on nerve tissue healing after sciatic nerve crush injury in rats.
20. Zhang, Q., Burrell, J. C., Zeng, J., Motiwala, F. I., Shi, S., Cullen, D. K., & Le, A. D. (2022). Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves. Stem cell research & therapy, 13(1), 1-18.

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