Histological comparison of three calcium silicate materials in the Pulpotomy of rat molars

Main Article Content

Nikitha Suzanne Varghese
Karthik Ganesh Mohanraj
Ganesh Jeevanandan

Keywords

cost, calcium, molars, alternative

Abstract

Introduction: The importance of preservation of pulp tissue prolongs the vitality of the tooth. The introduction of calcium silicate cements has proven to increase the success rate of the treatment, but the increased cost of the material is a disadvantage. Thus the development of a new material which has a superior clinical effect and is safe and economical is of utmost importance.
Aim: The purpose of this research is to histologically evaluate a novel calcium silicate cement and correlate it to Biodentine and Mineral trioxide aggregate (MTA) as pulpotomy agents in a rat model.
Subjects and methods: A total of nine Wistar albino rats were divided into three groups equally. Pulpotomies were conducted on the caries free mandibular molars using the three calcium silicate cements- new calcium silicate cement, Biodentine, and MTA under general anaesthesia. Glass ionomer cement that had been resin-modified was used to seal access cavities. At 7, 14, and 28 days following surgery, pulpotomized teeth in both groups were examined histologically under a light microscope (10x).
Results: Over the course of the experimented time periods, the inflammatory cell response gradually decreased in all three groups.
Conclusions: The new calcium silicate cement has been shown to have a less inflammatory reaction. This new calcium silicate cement can be used as a cost-effective alternative pulpotomy agent in comparison to the commercially available cements.

Abstract 169 | PDF Downloads 164

References

1. Minic S, Florimond M, Sadoine J, Valot-Salengro A, Chaussain C, Renard E, et al. Evaluation of Pulp Repair after BiodentineTM Full Pulpotomy in a Rat Molar Model of Pulpitis. Biomedicines [Internet]. 2021 Jul 6;9(7). Available from: http://dx.doi.org/10.3390/biomedicines9070784
2. Gaudin A, Renard E, Hill M, Bouchet-Delbos L, Bienvenu-Louvet G, Farges JC, et al. Phenotypic analysis of immunocompetent cells in healthy human dental pulp. J Endod. 2015 May;41(5):621–7.
3. Izumi T, Kobayashi I, Okamura K, Sakai H. Immunohistochemical study on the immunocompetent cells of the pulp in human non-carious and carious teeth. Arch Oral Biol. 1995 Jul;40(7):609–14.
4. Ranly DM. Pulpotomy therapy in primary teeth: new modalities for old rationales. Pediatr Dent. 1994 Nov;16(6):403–9.
5. Garcia-Godoy F. Clinical evaluation of glutaraldehyde pulpotomies in primary teeth. Acta Odontol Pediatr. 1983 Dec;4(2):41–4.
6. Huth KC, Hajek-Al-Khatar N, Wolf P, Ilie N, Hickel R, Paschos E. Long-term effectiveness of four pulpotomy techniques: 3-year randomised controlled trial. Clin Oral Investig. 2012 Aug;16(4):1243–50.
7. Ketley CE, Goodman JR. Formocresol toxicity: is there a suitable alternative for pulpotomy of primary molars? Int J Paediatr Dent. 1991 Aug;1(2):67–72.
8. Abdul Salam TA, Varghese S, Shenoy RP. Prevalence and clinical parameters of cervical abrasion as a function of population, age, gender, and toothbrushing habits: A systematic review. WORLD. 2019;10(6):471.
9. Daltoé MO, Paula-Silva FWG, Faccioli LH, Gatón-Hernández PM, De Rossi A, Bezerra Silva LA. Expression of Mineralization Markers during Pulp Response to Biodentine and Mineral Trioxide Aggregate. J Endod. 2016 Apr;42(4):596–603.
10. Teja KV, Ramesh S. Nonsurgical Management of Strip Perforation Using Platelet-Rich Fibrin and MTA by Matrix Concept - A Case Report with One Year Follow-up. Contemp Clin Dent. 2021 Mar 20;12(1):84–7.
11. Smaïl-Faugeron V, Glenny AM, Courson F, Durieux P, Muller-Bolla M, Fron Chabouis H. Pulp treatment for extensive decay in primary teeth. Cochrane Database Syst Rev. 2018 May 31;5(5):CD003220.
12. Yildiz E, Tosun G. Evaluation of formocresol, calcium hydroxide, ferric sulfate, and MTA primary molar pulpotomies. Eur J Dent. 2014 Apr;8(2):234–40.
13. Kavarthapu A, Thamaraiselvan M. Assessing the variation in course and position of inferior alveolar nerve among south Indian population: A cone beam computed tomographic study. Indian J Dent Res. 2018 Jul-Aug;29(4):405–9.
14. Akshayaa L, Ravindran V, Madhulaxmi M. Choice Of Tricalcium Silicate Cements Among Children For Pulp Capping-A Retrospective Study. Int J Dentistry Oral Sci. 2021;8(9):4527–30.
15. Mohanty S, Ramesh S. Comparing quality and quantity of dentin bridge formed using mineral trioxide aggregate, biodentine, and Propolis: A double-blinded randomized controlled clinical trial. World J Dent. 2020 Dec 14;11(5):373–9.
16. Bramhecha A, Sandhya R. Analysis of Marginal Gap between Dentin and Biodentine on Irrigation with 3% Sodium Hypochlorite-An In Vitro study. Int J Den-tistry Oral Sci. 2021;8(8):3766–9.
17. Teja KV, Kumar ILS, Ramesh S, Vasundhara KA, Others. Management of external root resorption with Biodentine and platelet-rich fibrin matrix: A case report with 3 year follow up. Saudi Endodontic Journal. 2021;11(3):405.
18. Panchal V, Jeevanandan G, Subramanian EMG. Comparison of instrumentation time and obturation quality between hand K-file, H-files, and rotary Kedo-S in root canal treatment of primary teeth: A randomized controlled trial. J Indian Soc Pedod Prev Dent. 2019;37(1):75.
19. Shahi S, Rahimi S, Yavari HR, Mokhtari H, Roshangar L, Abasi MM, et al. Effect of mineral trioxide aggregates and Portland cements on inflammatory cells. J Endod. 2010 May;36(5):899–903.
20. Tziafas D, Kodonas K. Dentinogenic Specificity in the Preclinical Evaluation of Vital Pulp Treatment Strategies: A Critical Review. Dent J. 2015 Nov 27;3(4):133–56.
21. Silva GF, Tanomaru-Filho M, Bernardi MIB, Guerreiro-Tanomaru JM, Cerri PS. Niobium pentoxide as radiopacifying agent of calcium silicate-based material: evaluation of physicochemical and biological properties. Clin Oral Investig. 2015 Nov;19(8):2015–25.
22. Dammaschke T. Rat molar teeth as a study model for direct pulp capping research in dentistry. Lab Anim. 2010 Jan;44(1):1–6.
23. Salako N, Joseph B, Ritwik P, Salonen J, John P, Junaid TA. Comparison of bioactive glass, mineral trioxide aggregate, ferric sulfate, and formocresol as pulpotomy agents in rat molar. Dent Traumatol. 2003 Dec;19(6):314–20.
24. Ratnakumari N, Thomas B. A Histopathological Comparison of Pulpal Response to Chitra-CPC and Formocresol used as Pulpotomy Agents in Primary Teeth: A Clinical Trial. Int J Clin Pediatr Dent. 2012 Jan;5(1):6–13.
25. Anbu RT, Suresh V, Gounder R, Kannan A. Comparison of the Efficacy of Three Different Bone Regeneration Materials: An Animal Study. Eur J Dent. 2019 Feb;13(1):22–8.
26. Ganapathy D, Others. AWARENESS ON CHELATING AGENTS USED IN RCT AMONG DENTAL STUDENTS. International Journal of Early Childhood Special Education [Internet]. 2022;14(3). Available from: https://search.ebscohost.com/login.aspx?direct=true&profile=ehost&scope=site&authtype=crawler&jrnl=13085581&AN=162720711&h=2XOMGqR%2BzisGdfskW0DzmX7l23h62iwgWtnF0eJj4%2FUMPHy0COAteZUzysomThZJ9gtUJc9eEEiSKMKn0nKWYQ%3D%3D&crl=c

Most read articles by the same author(s)

1 2 > >>