COMPARATIVE STUDY OF BIOFILM FORMATION ON DIFFERENT RESTORATIVE MATERIALS
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Abstract
Background: Biofilm growth on dental material restorations is a significant concern, as it can lead to secondary infections, caries, and periodontal disease. Biofilms have the capacity to adhere to various surfaces, a property that is determined by the material.
Objective: To identify the biofilm on composite resin, zirconia, and acrylic resin after 48 hours of exposure in the presence of bacteria, as well as the influence of roughness on bacterial adhesion.
Methods: Streptococcus mutans was used in this comparative in vitro study to ascertain biofilm formation on three restorative materials. The surface roughness was determined using the profilometer, and the growth of the biofilm was determined using crystal violet stain and spectrophotometer analysis.
Results: Acrylic resin was noted to exhibit the highest biofilm-forming properties, followed by composite resin with the least biofilm-forming properties in the zirconia. There were some statistically significant differences between the materials (p < 0.05).
Conclusion: Biofilm formation is significantly influenced by surface roughness; the smoother the surface, such as zirconia, the less bacterial adhesion occurs.
References
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Sobia Akhter
Senior Lecturer, Department of Biochemistry, Baqai Medical University, Karachi, Pakistan
Omer Hafeez Kaleem
Consultant Orthodontist, Affiliation Private Dental Clinic, Pakistan
Nimrah Khalil
Senior Registrar, Quetta Institute of Medical Sciences, Pakistan
Abdul Qadir
Assistant Professor Dental Materials, Foundation University Islamabad, Pakistan
Shah Salman Khan
Associate Professor, Bashir Dental College, Pakistan
Syed Muhammad Faran Ali
BDS, MSc, Assistant Professor and HOD, Department of Oral Pathology, Sir Syed College of Medical Science, Karachi, Pakistan