CHARACTERIZATION OF BIOPOLYMERIC ENCAPSULATION SYSTEM FOR IMPROVED SURVIVAL OF BIFIDOBACTERIUM LONGUM BL-101
Main Article Content
Keywords
Microencapsulation, Human like collagen, Bifidobacterium longum BL-101, Sodium alginate, Survival in simulated gastrointestinal conditions.
Abstract
Human like collagen and sodium alginate were used to prepare microspheres for the effective delivery of Bifidobacterium longum BL-101 in humans and animals. The microspheres were prepared through the method of extrusion. High survival rate of probiotics was detected after 21 days of storage, while the release rate of encapsulated B. longum BL-101 was also higher than non-encapsulated. The survival of probiotics is crucial to deliver benefits to host (either human or animal). It was observed that encapsulated B. longum BL-101 exhibited greater survival rates in simulated gastric and intestinal conditions in contrast with non-encapsulated probiotics. However, the encapsulation efficiency of human like collages and sodium alginate microspheres was 93.2±1.2% when 2.5 % HLC and 1.5 % sodium alginate were used.
References
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21. Wang, N., T. Li and P. Han. 2016. The effect of tianmai xiaoke pian on insulin resistance through PI3-K/AKT signal pathway. J. Diabetes Res. 16:1-8.
22. Xu, M., F. Gagne-Bourque, M.J. Dumont and S. Jabaji. 2016. Encapsulation of Lactobacillus casei ATCC 393 cells and evaluation of their survival after freeze-drying, storage and under gastrointestinal conditions. J. Food Eng. 168:52-59.
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24. Yasmin, I., M. Saeed, I. Pasha and M.A. Zia. 2019. Development of whey protein concentrate-pectin-alginate based delivery system to improve survival of B. longum BL-05 in simulated gastrointestinal conditions. Probiotics Antimicrob. Proteins. 11:413-426.
25. Yoo, S.H., Y.B. Song, P.S. Chang and H.G. Lee. 2006. Microencapsulation of α-tocopherol using sodium alginate and its controlled release properties. Int. J. Biol. Macromol. 38(1):25-30.
26. Zou, Q., J. Zhao, X. Liu, F. Tian, H.P. Zhang, H. Zhang and W. Chen. 2011. Microencapsulation of Bifidobacterium bifidum F‐35 in reinforced alginate microspheres prepared by emulsification/internal gelation. Int. J. Food Sci. Tech. 46(8):1672-1678.
2. Anee, I.J., S. Aslam, R.A. Begum, R.M. Shahjahan and A.M. Khandaker. 2021. The role of probiotics on animal health and nutrition. J. Basic Appl. Zool. 82:1-16.
3. Annan, N., A. Borza and L.T. Hansen. 2008. Encapsulation in alginate-coated gelatin
microspheres improve survival of the probiotic Bifidobacterium adolescentis 15703
during exposure to simulated gastro-intestinal conditions. Food Res. Int. 41:184-193.
4. Chang, L., P. Wang, S. Sun, Z. Shen and X. Jiang. 2021. A synbiotic marine oligosaccharide microcapsules for enhancing Bifidobacterium longum survivability and regulatory on intestinal probiotics. Int. J. Food Sci. Technol. 56:362-373.
5. Doherty, S.B., V.L. Gee, R.P. Ross, C. Stanton, G.F. Fitzgerald and A. Brodkorb. 2011. Development and characterization of whey protein micro-beads as potential matrices for probiotic protection. Food Hydrocoll. 25(6):1604-1617.
6. Falah, F., A. Vasiee, B.A. Behbahani, F.T. Yazdi, S. Moradi, S.A. Mortazavi and S. Roshanak. 2019. Evaluation of adherence and anti-infective properties of probiotic Lactobacillus fermentum strain 4-17 against Escherichia coli causing urinary tract infection in humans. Microb. Pathog. 131:246-253.
7. Gebara, C., K.S. Chaves, M.C.E. Ribeiro, F.N. Souza, C.R. Grosso and M.L. Gigante. 2013. Viability of Lactobacillus acidophilus La5 in pectin-whey protein microparticles
during exposure to simulated gastrointestinal conditions. Food Res. Int. 51:872-878.
8. Georgieva, R., L. Yocheva, L. Tserovska, G. Zhelezova, N. Stefanova, A. Atanasova, A. Danguleva, G. Ivanova, N. Karapetkov, N. Rumyan and E. Karaivanova. 2015. Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures. Biotechnol. Biotechnol. Equip. 29(1):84-91.
9. Haghshenas, B., N. Abdullah, Y. Nami, D. Radiah, R. Rosli and A.Y. Khosroushahi. 2015.
a. Microencapsulation of probiotic bacteria Lactobacillus plantarum 15HN using alginate‐
b. psyllium‐fenugreek polymeric blends. J. Appl. Microbiol. 118:1048-1057.
10. Kataria, A., S.C. Achi and P.M. Halami. 2018. Effect of encapsulation on viability of Bifidobacterium longum CFR815j and physiochemical properties of ice cream. Ind. J. Microbiol. 58:248-251.
11. Krausova, G., I. Hyrslova and I. Hynstova. 2019. In vitro evaluation of adhesion capacity, hydrophobicity, and auto-aggregation of newly isolated potential probiotic strains. Ferment. 5:100-111.
12. Lee, J., Yun, H. S., Cho, K. W., Oh, S., Kim, S. H., Chun, T., and Whang, K. Y. 2011. Evaluation of probiotic characteristics of newly isolated Lactobacillus spp.: immune modulation and longevity. Int. J. Food Microbiol. 148:80-86.
13. Lee, K.Y. and T.R. Heo. 2000. Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution. Appl. Environ. Microbiol. 66(2):869-873.
14. Moghanjougi, Z.M., M.R. Bari, M.A. Khaledabad, S. Amiri and H. Almasi. 2021. Microencapsulation of Lactobacillus acidophilus LA‐5 and Bifidobacterium animalis BB‐12 in pectin and sodium alginate: a comparative study on viability, stability, and structure. Food Sci. Nut. 9(9):5103-5111.
15. Prasanna, P.H.P. and D. Charalampopoulos. 2018. Encapsulation of Bifidobacterium longum in alginate-dairy matrices and survival in simulated gastrointestinal conditions, refrigeration, cow milk and goat milk. Food biosci. 21:72-79.
16. Rajam, R., P. Karthik, S. Parthasarathi, G. Joseph and C. Anandharamakrishnan. 2012. Effect of whey protein–alginate wall systems on survival of microencapsulated Lactobacillus plantarum in simulated gastrointestinal conditions. J. Funct. Foods. 4:891-898.
17. Schell, M.A., M. Karmirantzou, B. Snel, D. Vilanova, B. Berger, G. Pessi, M.C. Zwahlen, F. Desiere, P. Bork, M. Delley, R.D. Pridmore and F. Arigoni. 2002. The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. Proc. Natl. Acad. Sci. 99(22):14422-14427.
18. Shi, L.E., Z.H. Li, Z.L. Zhang, T.T. Zhang, W.M. Yu, M.L. Zhou and Z.X. Tang. 2013. Encapsulation of Lactobacillus bulgaricus in carrageenan-locust bean gum coated milk microspheres with double layer structure. Food Sci. Technol. 54:147-151.
19. Su, R., X.L. Zhu, D.D. Fan, Y. Mi, C.Y. Yang and X. Jia. 2011. Encapsulation of probiotic Bifidobacterium longum BIOMA 5920 with alginate–human-like collagen and evaluation of survival in simulated gastrointestinal conditions. Int. J. Biol. Macromol. 49:979-984.
20. Thantsha, M.S., T.E. Cloete, F.S. Moolman and P.W. Labuschagne. 2009. Supercritical carbon dioxide interpolymer complexes improve survival of B. longum Bb-46 in simulated gastrointestinal fluids. Int. J. Food Microbiol. 129(1):88-92.
21. Wang, N., T. Li and P. Han. 2016. The effect of tianmai xiaoke pian on insulin resistance through PI3-K/AKT signal pathway. J. Diabetes Res. 16:1-8.
22. Xu, M., F. Gagne-Bourque, M.J. Dumont and S. Jabaji. 2016. Encapsulation of Lactobacillus casei ATCC 393 cells and evaluation of their survival after freeze-drying, storage and under gastrointestinal conditions. J. Food Eng. 168:52-59.
23. Yang, Q. and Z. Wu. 2023. Gut probiotics and health of dogs and cats: benefits applications and underlying mechanisms. Microorganisms. 11(10):2452-2459.
24. Yasmin, I., M. Saeed, I. Pasha and M.A. Zia. 2019. Development of whey protein concentrate-pectin-alginate based delivery system to improve survival of B. longum BL-05 in simulated gastrointestinal conditions. Probiotics Antimicrob. Proteins. 11:413-426.
25. Yoo, S.H., Y.B. Song, P.S. Chang and H.G. Lee. 2006. Microencapsulation of α-tocopherol using sodium alginate and its controlled release properties. Int. J. Biol. Macromol. 38(1):25-30.
26. Zou, Q., J. Zhao, X. Liu, F. Tian, H.P. Zhang, H. Zhang and W. Chen. 2011. Microencapsulation of Bifidobacterium bifidum F‐35 in reinforced alginate microspheres prepared by emulsification/internal gelation. Int. J. Food Sci. Tech. 46(8):1672-1678.
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Published
Aug 17, 2024
Article Details
How to Cite
Sabahat Amin, Muhammad Saeed, Allah Rakha, & Muhammad Naeem Faisal. (2024). CHARACTERIZATION OF BIOPOLYMERIC ENCAPSULATION SYSTEM FOR IMPROVED SURVIVAL OF BIFIDOBACTERIUM LONGUM BL-101. Journal of Population Therapeutics and Clinical Pharmacology, 31(8), 3805-3813. https://doi.org/10.53555/3thcaw03
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Author Biographies
Sabahat Amin
National Institute of Food Science and Technology, University Road, University of Agriculture, Faisalabad-38000 Punjab, Pakistan.
Muhammad Saeed
National Institute of Food Science and Technology, University Road, University of Agriculture, Faisalabad-38000 Punjab, Pakistan.
Allah Rakha
National Institute of Food Science and Technology, University Road, University of Agriculture, Faisalabad-38000 Punjab, Pakistan.
Muhammad Naeem Faisal
Institute of Pharmacy, Physiology, and Pharmacology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan.
How to Cite
Sabahat Amin, Muhammad Saeed, Allah Rakha, & Muhammad Naeem Faisal. (2024). CHARACTERIZATION OF BIOPOLYMERIC ENCAPSULATION SYSTEM FOR IMPROVED SURVIVAL OF BIFIDOBACTERIUM LONGUM BL-101. Journal of Population Therapeutics and Clinical Pharmacology, 31(8), 3805-3813. https://doi.org/10.53555/3thcaw03