EXPLORING THE ANTIOXIDANT POWER OF EUCALYPTUS CAMALDULENSIS (SUFAIDA) AND ITS POTENTIAL ROLE IN AFLATOXINS DEGRADATION
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Keywords
Eucalyptus Camaldulensis (Sufaida) Aflatoxins, Antioxidant, HPLC, TLC, Degradation of aflatoxins
Abstract
Aflatoxins are secondary metabolite compound produced by fungus Aspergillus flavus, Aspergillus nomius and Aspergillus parasiticus. Aflatoxin degraded by the extract of Eucalyptus camaldulensis. The extract leaf of Eucalyptus Camaldulensis analyzed for determination of antioxidant and degradation of aflatoxin. The identification and quantification degaradation of aflatoxin analyzed by Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography techniques (HPLC) respectively. In this research, the mean values of water, Acetone, Hexane and Methanol extract of DPPH, FRAP and TPC were (13.41 %, 58.51%, and 27.72%), (68 mg GAE /100g, 466.5 mg GAE /100g, 180 mg GAE /100g, and 450 mg GAE /100g) and (268.78 mg GAE /100g, 269.88 mg GAE /100g, 68.22 mg GAE /100g and 276 mg GAE /100g) respectively. The mean of degaradation AFB1, AFB2, AFG1, AFG2 and TAF) were 3.05±0.29 µg/kg, 2.15±0.17 µg/kg, 4.21±0.25 µg/kg, 2.88±0.20 µg/kg and 12.29±0.09µg/kg respectively. The removal % of total Aflatoxin by Eucalyptus camaldulensis was 95.08 %.
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Muhammad Naeem Zubairi
Government of Pakistan, Islamabad Food Authority, Islamabad Capital Territory, Pakistan.
Muhammad Khurram Afzal
Department of Human Nutrition, Faculty of food science and nutrition Bahauddin Zakariya University Multan Pakistan.
Hafiz Muhammad Irfan Manzoor
Department of Agriculture and Food Technology, Karakoram International University, Gilgit, Pakistan.
Talha Bin Iqbal
Department of Food Science and Technology, Faculty of food science and nutrition Bahauddin Zakariya University Multan Pakistan.
Nayab Rao
Government of Pakistan Islamabad Food Authority Islamabad Capital Territory, Pakistan.
Naeem Sarwar
Department of Human Nutrition Faculty of food science and nutrition Bahauddin Zakariya University Multan, Pakistan.
Liaqat Ali
Department of forestry range and wildlife management, Karakorum International University, Gilgit, Pakistan.