PHYTOCHEMICALS ANALYSIS VIA GC-MS, ASSESSMENT OF ANTI-INFLAMMATORY, ANTI-BACTERIAL, AND ANTI- FUNGAL PROPERTIES OF CASSIA FISTULA POD-BASED FUNCTIONAL TEA

Abstract

Cassia fistula Linn, also known as Pakistan Amaltas and Indian Laburnum, is a tropical plant used in traditional medicine for treating syphilis, tumors, burns, constipation, and skin disorders. The study aimed to ascertain the Cassia fistula Linn pods' zone of inhibition against bacterial strains and inflammation, in addition to assessing the pods' potential as antibacterial and ant-inflammatory agents. The hydroalcohol extracts' possible antibacterial efficacy against important bacterial and fungal species for medicine was evaluated. The antibacterial activity of Cassia fistula’s pod extracts (5, 25, 50, 100, and 250 μg/ml) against a variety of pathogenic bacteria and fungal species was assessed using the agar disc diffusion method. The extracts showed a significant decrease of inflammation and bacterial growth against the tested species. The microbiological activity of Cassia fistula’s pods has been associated with a number of secondary metabolites. The identification of naturally occurring bioactive chemicals in these plants may open the door to new directions in pharmacological research. The zone of inhibition was compared to a number of standards, such as ampicillin, ciprofloxacin, norfloxacin, chloramphenicol, nystatin, and griseofulvin.  Anti-inflammatory teat showed a typical immunological response in LPS-stimulated cells, with TNF- and NO levels of 85.9% and 76%, respectively. There is more potential for hydrocortisone and Cassia fistula’s pods to reduce particular inflammatory markers. The antibacterial test showed growth inhibition zones against S. pyogenes, S. aureus, E. coli, and P. aeruginosa at dosages ranging from 5 to 25 µg/ml. The amount of tannin, alkaloids, saponin, and total phenolic content were all reduced by the Cassia fistula’s pod extraction. Immunomodulatory investigations revealed a greater blood antibody titer in the extract-fed group. The GC-MC technique was utilized to analyze phytochemical compounds in the Cassia fistula pod extract, revealing thymine, butanoic acid, 2-methyl-, 2- methylpropyl ester, furancarboxaldehyde, pentanoic acid, 1,1 dimethylethyl ester, 5-Acetoxymethyl- 2-furaldehyde, butanoic acid, Valeric acid, 2,4;3,5-Dimethylene-1-iditol, Vitamin E, n-Hexadecanoic acid, Myo-Inositol, 4-C-methyl, oleic acid, and a-sitosterol.