EVALUATION OF PHYTOCHEMICAL ANALYSIS AND IN-VITRO EFFECT OF TRIBULUS TERRESTRIS EXTRACT ON SEMEN QUALITY

Main Article Content

Syed Aatif Hussain
Syed Muhammad Ali Shah
Abid Rashid
Sultan Ayaz
Rizwan Asif

Keywords

Male infertility, Herbal Medicine, T. terrestris, Anti-oxidant potential, Sperm motility

Abstract

Approximately half of human infertility cases are attributed to male infertility and natural herbs are promising medicines used to cure infertility. Worldwide, it is accepted that over 80% of the population of developing countries directly or indirectly relies on herbal medicine. This study was conducted to check the phytochemical analysis and effect of Tribulus terrestris extract on semen quality. A preliminary qualitative phytochemical analysis and quantitative analysis were performed to determine total phenolic contents (TPC) and total flavonoids contents (TFC) after ethanolic extract preparation of T. terrestris. The antioxidant potential of the T. terrestris sample was determined by DPPH radical scavenging assay. In-vitro effects of T. terrestris on sperm quality were also evaluated. A preliminary phytochemical analysis revealed bioactive compounds like glycosides, tannins, alkaloids, flavonoids, terpenoids, and quinones. Moreover, significant TPC and TFC were also found. Remarkable positive changes in sperm quality like motility and viability were observed after incubation with T. terrestris extract (50 ug/ml) for 60 to 120 minutes as compared to control group. T terrestris could affect male fertility capacity and this research could be a big success in curing male infertility using alternative herbal medicine without having side effects and being cost effective.

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