EXPLORATION OF FIFTY LOCAL PAKISTANI MEDICINAL PLANTS FOR ACETYLCHOLINESTERASE (ACHE) INHIBITORY ACTIVITY
Main Article Content
Keywords
Acetylcholinesterase inhibition, Alzheimer’s disease, Pakistani medicinal plants, phytochemical.
Abstract
Objective: The principal aim of the current study was to estimate the Acetylcholinesterase (AChE) inhibition activity of crude hydro-methanolic extracts of fifty selected local medicinal plants; traditionally known for therapeutic properties. The top four plant extracts that showed promising AChE inhibition activity were further analyzed for antioxidant activity and phytochemical composition (total phenolic, total flavonoid, total tannins, total terpenoids, and total alkaloids). Methods: All the plant extracts were made in 80% methanol. The AChE inhibition activity was determined by a micro-plate assay and the antioxidant potential was calculated by DPPH free radical scavenging assay. Plant extracts having ≥50% ACHE inhibitory activity were further analyzed for phytochemicals, total phenols, total tannins, total flavonoids, total terpenoids, total alkaloids. Results: The results highlighted that out of all fifty selected plants only four possess >50% AChE inhibition potential, that are Quercus infectoria (87.6%), Flacourtia jangomas (66.6%), Peganum harmala (52.8%) and Solanum pseudocapsicum (50.3%) The P. harmala have the least (37.9%) and Q. infectoria possess the highest (90.6%) antioxidant activity, which is justified by the amount of phenolics and flavonoids compounds present in these plants. Phytochemicals study revealed that Q. infectoria possess higher flavonoid and alkaloid content. Whereas, S. pseudocapsicum possess higher phenolic and terpenoid content. Additionally, P. harmala possess high amount of tannins. On the contrary, this plant possess low content of phenolics, terpenoids, flavonoids, and alkaloids. Conclusion: This study concludes Q. infectoria induced significant AChE inhibitory activity as well as phytochemical properties. Thus, this plant could be promising for the development of new therapeutic agents. Additionally, Flacourtia jangomas was reported as new potent AChE inhibitor that encourage further studies that can lead to the development of new agents that might be used in the treatment of Alzheimer’s disease.
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Khurshid Anwar
FoodOmics laboratory, Department of Food Science & Technology, The University of Haripur, Khyber-Pakhtunkhwa, Pakistan
Sajjad Ali
Shaanxi Key Laboratory of Natural Products and Chemical Biology, College of Chemistry and Pharmacy, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China
Abdullah Bin Naseer
FoodOmics laboratory, Department of Food Science & Technology, The University of Haripur, Khyber-Pakhtunkhwa, Pakistan
Ammad Amin
FoodOmics laboratory, Department of Food Science & Technology, The University of Haripur, Khyber-Pakhtunkhwa, Pakistan
Zartaj Malik
FoodOmics laboratory, Department of Food Science & Technology, The University of Haripur, Khyber-Pakhtunkhwa, Pakistan
Inam-u-llah
FoodOmics laboratory, Department of Food Science & Technology, The University of Haripur, Khyber-Pakhtunkhwa, Pakistan
Muhammad Jahangir
Food Omics laboratory, Department of Food Science & Technology, The University of Haripur, Khyber-Pakhtunkhwa, Pakistan