EXPLORATION OF FIFTY LOCAL PAKISTANI MEDICINAL PLANTS FOR ACETYLCHOLINESTERASE (ACHE) INHIBITORY ACTIVITY

Main Article Content

Khurshid Anwar
Sajjad Ali
Abdullah Bin Naseer
Ammad Amin
Zartaj Malik
Inam-u-llah
Muhammad Jahangir

Keywords

Acetylcholinesterase inhibition, Alzheimer’s disease, Pakistani medicinal plants, phytochemical.

Abstract

Objective: The principal aim of the current study was to estimate the Acetylcholinesterase (AChE) inhibition activity of crude hydro-methanolic extracts of fifty selected local medicinal plants; traditionally known for therapeutic properties. The top four plant extracts that showed promising AChE inhibition activity were further analyzed for antioxidant activity and phytochemical composition (total phenolic, total flavonoid, total tannins, total terpenoids, and total alkaloids). Methods: All the plant extracts were made in 80% methanol. The AChE inhibition activity was determined by a micro-plate assay and the antioxidant potential was calculated by DPPH free radical scavenging assay. Plant extracts having ≥50% ACHE inhibitory activity were further analyzed for phytochemicals, total phenols, total tannins, total flavonoids, total terpenoids, total alkaloids. Results: The results highlighted that out of all fifty selected plants only four possess >50% AChE inhibition potential, that are Quercus infectoria (87.6%), Flacourtia jangomas (66.6%), Peganum harmala (52.8%) and Solanum pseudocapsicum (50.3%) The P. harmala have the least (37.9%) and Q. infectoria possess the highest (90.6%) antioxidant activity, which is justified by the amount of phenolics and flavonoids compounds present in these plants. Phytochemicals study revealed that Q. infectoria possess higher flavonoid and alkaloid content. Whereas, S. pseudocapsicum possess higher phenolic and terpenoid content. Additionally, P. harmala possess high amount of tannins. On the contrary, this plant possess low content of phenolics, terpenoids, flavonoids, and alkaloids. Conclusion: This study concludes Q. infectoria induced significant AChE inhibitory activity as well as phytochemical properties. Thus, this plant could be promising for the development of new therapeutic agents. Additionally, Flacourtia jangomas was reported as new potent AChE inhibitor that encourage further studies that can lead to the development of new agents that might be used in the treatment of Alzheimer’s disease.

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References

1. Adhami, H.-R., Farsam, H. and Krenn, L., 2011. Screening of Medicinal Plants from Iranian Traditional Medicine for Acetylcholinesterase Inhibition. Phytotherapy Research. 25, 1148-1152.
2. Aliero, A., Asekun, O., Grierson, D. and Afolayan, A., 2006a. Chemical composition of the hexane extract from the leaves of Solanum pseudocapsicum.
3. Aliero, A., Grierson, D. and Afolayan, A., 2006b. Antifungal activity of Solanum pseudocapsicum. Res J Bot. 1, 129-33.
4. Aqil, F., Ahmad, I. and Mehmood, Z., 2006. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish journal of Biology. 30, 177-183.
5. Asem, N., Abdul Gapar, N. A., Abd Hapit, N. H. and Omar, E. A., 2020. Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract. Journal of Apicultural Research. 59, 437-442.
6. Asgarpanah, J. and Ramezanloo, F., 2012. Chemistry, pharmacology and medicinal properties of Peganum harmala L. African Journal of Pharmacy and Pharmacology. 6, 1573-1580.
7. Baba, S. A. and Malik, S. A., 2015. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science. 9, 449-454.
8. Badami, S., Manohara Reddy, S., Kumar, E., Vijayan, P. and Suresh, B., 2003. Antitumor activity of total alkaloid fraction of Solanum pseudocapsicum leaves. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 17, 1001-1004.
9. Badami, S., Prakash, O., Dongre, S. and Suresh, B., 2005. In vitro antioxidant properties of Solanum pseudocapsicum leaf extracts. Indian journal of pharmacology. 37, 251.
10. Das, S., Dewan, N., Das, K. J. and Kalita, D., 2017. Preliminary phytochemical, antioxidant and antimicrobial studies of Flacourtia jangomas fruits. Int J Curr Pharm Res. 9, 86-91.
11. Dastagir, G. and Hussain, F., 2014. Cytotoxic activity of plants of family Zygophyllaceae and Euphorbiaceae. Pak J Pharm Sci. 27, 801-805.
12. Dastagir, G., Hussain, F. and Khan, A. A., 2012. Antibacterial activity of some selected plants of family Zygophyllaceae and Euphorbiaceae. Journal of Medicinal Plants Research. 6, 5360-5368.
13. Dubey, N., Pandey, V. and Tewari, S., 2013. Antioxidant potential and phytochemical composition of unripe fruits of Flacourtia jangomas (Lour.) Raeusch. Medicinal Plants-International Journal of Phytomedicines and Related Industries. 5, 164-167.
14. Dutta, B. and Borah, N., 2017. Studies on nutraceutical properties of Flacourtia jangomas fruits in Assam, India. Journal of Medicinal Plants. 5, 50-53.
15. Fazal, H., Ahmad, N. and Khan, M. A., 2011. Physicochemical, phytochemical evaluation and DPPH-scavenging antioxidant potential in medicinal plants used for herbal formulation in Pakistan. Pak. J. Bot. 43, 63-67.
16. García, M. E., Borioni, J. L., Cavallaro, V., Puiatti, M., Pierini, A. B., Murray, A. P. and Peñéñory, A. B., 2015. Solanocapsine derivatives as potential inhibitors of acetylcholinesterase: Synthesis, molecular docking and biological studies. Steroids. 104, 95-110.
17. Gholamhoseinian, A., Moradi, M. and Sharifi-Far, F., 2009. Screening the methanol extracts of some Iranian plants for acetylcholinesterase inhibitory activity. Research in pharmaceutical sciences. 4, 105.
18. Ghorai, N., Chakraborty, S., Gucchait, S., Saha, S. K. and Biswas, S., 2012. Estimation of total Terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent.
19. Harman-Ware, A. E., Sykes, R., Peter, G. F. and Davis, M., 2016. Determination of terpenoid content in pine by organic solvent extraction and fast-GC analysis. Frontiers in Energy Research. 4, 2.
20. Ingkaninan, K., Temkitthawon, P., Chuenchom, K., Yuyaem, T. and Thongnoi, W., 2003. Screening for acetylcholinesterase inhibitory activity in plants used in Thai traditional rejuvenating and neurotonic remedies. Journal of ethnopharmacology. 89, 261-264.
21. Jahangir, M., Abdel-Farid, I. B., Choi, Y. H. and Verpoorte, R., 2008. Metal ion-inducing metabolite accumulation in Brassica rapa. Journal of plant physiology. 165, 1429-1437.
22. John, B., Sulaiman, C., George, S. and Reddy, V., 2014. Spectrophotometric estimation of total alkaloids in selected Justicia species. International journal of pharmacy and pharmaceutical sciences. 6, 647-648.
23. Kaur, G., Hamid, H., Ali, A., Alam, M. S. and Athar, M., 2004. Antiinflammatory evaluation of alcoholic extract of galls of Quercus infectoria. Journal of ethnopharmacology. 90, 285-292.
24. Kumar, S., Yadav, M., Yadav, A. and Yadav, J., 2017. Impact of spatial and climatic conditions on phytochemical diversity and in vitro antioxidant activity of Indian Aloe vera (L.) Burm. f. South African journal of botany. 111, 50-59.
25. Lamchouri, F., Settaf, A., Cherrah, Y., Zemzami, M., Lyoussi, B., Zaid, A., Atif, N. and Hassar, M., 1999. Antitumour principles from Peganum harmala seeds. Therapie. 54, 753-758.
26. Lim, T., 2012. Quercus infectoria. Edible Medicinal And Non-Medicinal Plants. Springer.
27. Lim, T., 2013. Flacourtia jangomas. Edible Medicinal And Non-Medicinal Plants. Springer.
28. Luis, L. G., Barreto, Â., Trindade, T., Soares, A. M. and Oliveira, M., 2016. Effects of emerging contaminants on neurotransmission and biotransformation in marine organisms—an in vitro approach. Marine pollution bulletin. 106, 236-244.
29. Malik, S., Ahmad, M. and Khan, F., 2017. Qualtitative and quantitative estimation of terpenoid contents in some important plants of punjab, pakistan. Pakistan Journal of Science. 69, 150.
30. Mohammed, S. and Manan, F., 2015. Analysis of total phenolics, tannins and flavonoids from Moringa oleifera seed extract. Journal of Chemical and Pharmaceutical Research. 7, 132-135.
31. Murray, A. P., Faraoni, M. B., Castro, M. J., Alza, N. P. and Cavallaro, V., 2013. Natural AChE inhibitors from plants and their contribution to Alzheimer’s disease therapy. Current neuropharmacology. 11, 388-413.
32. Nathan, S. S., Choi, M. Y., Seo, H. Y., Paik, C. H., Kalaivani, K. and Kim, J. D., 2008. Effect of azadirachtin on acetylcholinesterase (AChE) activity and histology of the brown planthopper Nilaparvata lugens (Stål). Ecotoxicology and Environmental Safety. 70, 244-250.
33. Niño, J., Hernández, J. A., Correa, Y. M. and Mosquera, O. M., 2006. In vitro inhibition of acetylcholinesterase by crude plant extracts from Colombian flora. Memórias do Instituto Oswaldo Cruz. 101, 783-785.
34. Obulesu, M. and Rao, D. M., 2011. Effect of plant extracts on Alzheimer’s disease: An insight into therapeutic avenues. Journal of neurosciences in rural practice. 2, 056-061.
35. Patro, S. K., Behera, P. C., Kumar, P. M., Sasmal, D., Padhy, R. K. and Dash, S., 2013. Pharmacological Review of Flacourtia sepiaria (Ruxb.). Sch. Acad. J. Pharm. 2, 89-93.
36. Penumala, M., Zinka, R. B., Shaik, J. B. and Amooru Gangaiah, D., 2017. In vitro screening of three Indian medicinal plants for their phytochemicals, anticholinesterase, antiglucosidase, antioxidant, and neuroprotective effects. BioMed research international. 2017.
37. Pramila, D., Xavier, R., Marimuthu, K., Kathiresan, S., Khoo, M., Senthilkumar, M., Sathya, K. and Sreeramanan, S., 2012. Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (Mentha piperita: Lamiaceae). J Med Plants Res. 6, 331-335.
38. Rhee, I. K., Appels, N., Luijendijk, T., Irth, H. and Verpoorte, R., 2003. Determining acetylcholinesterase inhibitory activity in plant extracts using a fluorimetric flow assay. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques. 14, 145-149.
39. Salamah, N. and Ningsih, D., Total alkaloid content in various fractions of Tabernaemonata sphaerocarpa Bl.(Jembirit) leaves. IOP Conference Series: Materials Science and Engineering, 2017. IOP Publishing, 012017.
40. Sanghvi, G. V., Koyani, R. D., Patil, V. S. and Rajput, K. S., 2011. Morpho-anatomy of Solanum pseudocapsicum. Revista Brasileira de Farmacognosia. 21, 11-15.
41. Sasi, S., Anjum, N. and Tripathi, Y., 2018. ETHNOMEDICINAL, PHYTOCHEMICAL AND PHARMACOLOGICAL ASPECTS OF FLACOURTIA JANGOMAS: A REVIEW.
42. Şenol, F. S., Şekeroğlu, N., Gezici, S., Kilic, E. and Orhan, İ. E., 2018. Neuroprotective potential of the fruit (acorn) from Quercus coccifera L. Turkish Journal of Agriculture and Forestry. 42, 82-87.
43. Shah, M. S., Najam-Ul-Haq, M., Shah, H. S., Rizvi, S. U. F. and Iqbal, J., 2018. Quinoline containing chalcone derivatives as cholinesterase inhibitors and their in silico modeling studies. Computational biology and chemistry. 76, 310-317.
44. Shahwar, D., Rehman, S. U. and Raza, M. A., 2010. Acetyl cholinesterase inhibition potential and antioxidant activities of ferulic acid isolated from Impatiens bicolor Linn. Journal of medicinal plants research. 4, 260-266.
45. Shahwar, D., Ullah, S., Raza, M. A., Sana, U., Yasmeen, A., Ghafoor, S. and Ahmad, N., 2011. Acetylcholine esterase and antioxidant potential of some members of Asteraceae and Euphorbiaceae. Journal of Medicinal Plants Research. 5, 7011-7016.
46. Shamsa, F., Monsef, H., Ghamooshi, R. and Verdian-Rizi, M., 2008. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci. 32, 17-20.
47. Steel, R. G. D. and Torrie, J. H., 1980. Principles and procedures of statistics, a biometrical approach, McGraw-Hill Kogakusha, Ltd.
48. Umachigi, S., Jayaveera, K., Kumar, C. A., Kumar, G. and Kumar, D. K., 2008. Studies on wound healing properties of Quercus infectoria. Tropical journal of Pharmaceutical research. 7, 913-919.
49. Vaidya, V., Mahendrakumar, C. and Bhise, K., 2013. Preliminary phytochemical screening of Quercus infectoria Oliv. for treatment of skin diseases. Journal of Medicinal Plants Research. 7, 2019-2027.
50. Vijayan, P., Kumar, S. V., Dhanaraj, S., Badami, S. and Suresh, B., 2002. In vitro cytotoxicity and anti-tumor properties of the total alkaloid fraction of unripe fruits of Solanum pseudocapsicum. Pharmaceutical biology. 40, 456-460.
51. Vinutha, B., Prashanth, D., Salma, K., Sreeja, S., Pratiti, D., Padmaja, R., Radhika, S., Amit, A., Venkateshwarlu, K. and Deepak, M., 2007. Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity. Journal of ethnopharmacology. 109, 359-363.
52. Yang, Y., Cheng, X., Liu, W., Chou, G., Wang, Z. and Wang, C., 2015. Potent AChE and BChE inhibitors isolated from seeds of Peganum harmala Linn by a bioassay-guided fractionation. Journal of ethnopharmacology. 168, 279-286.
53. Zheng, X.-Y., Zhang, L., Cheng, X.-M., Zhang, Z.-J., Wang, C.-H. and Wang, Z.-T., 2011. Identification of acetylcholinesterase inhibitors from seeds of plants of genus Peganum by thin-layer chromatography-bioautography. JPC-Journal of Planar Chromatography-Modern TLC. 24, 470-474.
54. Zheng, X.-Y., Zhang, Z.-J., Chou, G.-X., Wu, T., Cheng, X.-M., Wang, C.-H. and Wang, Z.-T., 2009. Acetylcholinesterase inhibitive activity-guided isolation of two new alkaloids from seeds of Peganum nigellastrum Bunge by an in vitro TLC- bioautographic assay. Archives of Pharmacal Research. 32, 1245-1251.

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