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Sudhair Abbas Bangash
Sadia Sardar
Qudsia Begum
Iqbal Nisa
Hamid Ali Khan
Atta Ullah
Obaid Hayat
Shaila Mehwish
Muhammad Akbar
Nain Taara Bukhari
Farah Shireen
Asia Hayat


Probiotic, nanoparticle, ZnO, antibacterial activity Lactobacillus fermentum, Saccharomyces cerevisiae.


Probiotics, such as Lactobacillus fermentum and Saccharomyces cerevisiae, play a crucial role in catalyzing the biogenesis of nano-minerals, offering a transformative solution to enhance nutrient absorption in farm animals and promote agricultural sustainability. This research aims to synthesize ZnO nanoparticles using Probiotic Lactobacillus fermentum and Saccharomyces cerevisiae, assessing their antibacterial efficacy against MDR pathogens and investigating the impact of Nano zinc supplementation on growth and blood parameters in goats. Utilizing analytical methods such as Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), and ultraviolet–visible spectroscopy (UV–Vis), the produced ZnO NPs were characterized, revealing distinct bonds and structural features. ZnO nanoparticles, highlighted through FTIR (emphasizing N-H stretching at 1450-1500 cm-1 and ZnO nanoparticle stretches at 400-800 cm-1), SEM (revealing micro-sized bioconjugates with dimensions of 100–120 nm), and XRD (confirming a hexagonal crystal structure at approximately 36.50 degrees), demonstrated significant antibacterial activity. Goats treated with ZnO nanoparticles exhibited substantial weight gain over 12 weeks (Initial BW: 29.2±0.48 kg; Final Body Weight - Treatment C: 40.99±0.58 kg, ZnO-1: 43.39±0.45 kg, ZnO-2: 44.05±0.43 kg), with limited physiological effects, except for notable variations in immunoglobulins (IgA, IgG) among treatment groups (p = 0.006 and p = 0.003, respectively). Notably, ZnO nanoparticles displayed antibacterial activity (ZnO-1 against E. cloacae: 13mm, A. baumannii: 12mm; ZnO-2 against S. aureus: 12mm, E. cloacae: 13mm, P. aeruginosa: 12mm), indicating their remarkable antimicrobial potency. This environmentally friendly and cost-effective approach to synthesizing ZnO NPs holds promise for diverse biomedical applications. 

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