Main Article Content

Muhammad Shahzad Khan
Mudassar Mazher
Qazi Adnan
Zafar Iqbal
Umer Shaukat
Hosh Muhammad Lashari
Abdul Quddoos
Mehran Khan
Shahzada Khurram
Umair Khurshid
Naheed Akhtar
Fahad Mehmood Khan
Marwah Rehman


Alkannatinctoria, DPPH, Elastometer, Total phenolic contents, total flavonoids contents, skin pores


Background: Herbs and plants with enormous levels of phenolic and flavonoid constituents are commonly utilized as antioxidant agents to reduce the impact of UV radiation on the skin and play a significant role in skin treatments.

Aims: Alkanna tinctoria extract's potential cosmetic benefits including reduction in melanin, erythema, sebum, anti-aging and sun protection effectswere investigated in this study, which also aimed to create a stable emulgel that contained 4% Alkanna tinctoria extract.

Objective: Alkanna tinctoria extract was examined and quantified for its phenol and flavonoid content in order to ascertain its cosmetic effects on human skin.

Methods: Alkanna tinctoria (AT) loaded o/w emulsion were combined with gel containing carbopol-940 as gelling agent to create the emulgel formulation. Same procedure and formulation was adopted for control emulgel without extract. For the duration of a 12-week storage period, an in vitro investigation was conducted to assess how color, liquefaction, pH and hardness changed. 13 healthy human volunteers' cheeks were treated with emulgel in order to assess its cosmetic benefits in comparison to control formulations. Utilizing the Mexameter, Elastometer, Sebumeter and Corneometer, and over the duration of the 12-week trial, facial parameters such as skin pigmentation, moisture content, redness, sebum, and skin elasticity were assessed.

Results: Alkanna tinctoria extract found to have 48.04 ± 0.0033 mg GAE/g of total phenolics, and flavonoids were 8.64 ± 0.0046 mg QE/g. Alkanna tinctoria emulgel significantly efficient at minimizing the sebum, small pores andlarge pores (p ≤ 0.05) while efficient to maximize skin’s elasticity and hydration as compared to control emulgel. Antioxidant activity by DPPH activity was 94.23±0.028.

Conclusion: The flavonoid and phenolic concentration of Alkanna tinctoria extract makes it a potent antioxidant that works to protect skin from photodamage and enhance skin conditions.

Abstract 168 | pdf Downloads 99


1. Lahlou M. The success of natural products in drug discovery. 2013.
2. Phillipson JD. Phytochemistry and medicinal plants. Phytochemistry. 2001;56(3):237-43.
3. Gurnani N, Mehta D, Gupta M, Mehta B. Natural products: source of potential drugs. Afr J Basic Appl Sci. 2014;6(6):171-86.
4. Khan RA. Natural products chemistry: The emerging trends and prospective goals. Saudi pharmaceutical journal. 2018;26(5):739-53.
5. Łopaciuk A, Łoboda M, editors. Global beauty industry trends in the 21st century. Management, knowledge and learning international conference; 2013.
6. Taeymans J, Clarys P, Barel AO. 55 use of food supplements as nutricosmetics in health and fitness. CRC Press; 2014. p. 583-96.
7. Akhtar N, Akhtar N. Development of stable tocopherol succinate‐loaded ethosomes to enhance transdermal permeation: In vitro and in vivo characterizations. J Cosmet Dermatol. 2022.
8. Rahmani‐Neishaboor E, Jallili R, Hartwell R, Leung V, Carr N, Ghahary A. Topical application of a film‐forming emulgel dressing that controls the release of stratifin and acetylsalicylic acid and improves/prevents hypertrophic scarring. Wound Repair Regen. 2013;21(1):55-65.
9. Khullar R, Kumar D, Seth N, Saini S. Formulation and evaluation of mefenamic acid emulgel for topical delivery. Saudi pharmaceutical journal. 2012;20(1):63-7.
10. Arioli G, Scaramelli M, Pillosu W. Topical therapy of acute skeletal muscle diseases. Results of a comparative study on piroxicam cream 1% versus diclofenac emulgel 1%. La Clinica Terapeutica. 1990;134(6):363-9.
11. Arshad W, Khan HMS, Akhtar N, Mohammad ISJBJoPS. Polymeric emulgel carrying Cinnamomum tamala extract: promising delivery system for potential topical applications. 2020;56.
12. Singla V, Saini S, Joshi B, Rana A. Emulgel: A new platform for topical drug delivery. International Journal of Pharma and Bio Sciences. 2012;3(1):485-98.
13. Dureja H, Kaushik D, Gupta M, Kumar V, Lather V. Cosmeceuticals: An emerging concept. Indian J Pharmacol. 2005;37(3):155.
14. Haneefa KM, Easo S, Hafsa P, Mohanta GP, Nayar C. Emulgel: An advanced review. Journal of pharmaceutical sciences and research. 2013;5(12):254.
15. Qureshi MS, Jamil QA, Akhtar N, Akhtar N. Formulation and characterization of Anacyclus Pyrethrum Emulgels and its in vitro and in vivo evaluation as cosmeceutical product. J Cosmet Dermatol. 2022.
16. Tung NH, Du G-J, Yuan C-S, Shoyama Y, Wang C-Z. Isolation and chemopreventive evaluation of novel naphthoquinone compounds from Alkanna tinctoria. Anti-Cancer Drugs. 2013;24(10).
17. Malik S, Bhushan S, Sharma M, Ahuja PS. Biotechnological approaches to the production of shikonins: a critical review with recent updates. Crit Rev Biotechnol. 2016;36(2):327-40.
18. Sabih Ozer M, Sarikurkcu C, Tepe B, Can S. Essential oil composition and antioxidant activities of alkanet (Alkanna tinctoria subsp. tinctoria). Food Sci Biotechnol. 2010;19(5):1177-83.
19. Ali S, Khan AS, Malik AU, Shaheen T, Shahid M. Pre-storage methionine treatment inhibits postharvest enzymatic browning of cold stored ‘Gola’litchi fruit. Postharvest Biol Technol. 2018;140:100-6.
20. Martínez-Las Heras R, Pinazo A, Heredia A, Andrés A. Evaluation studies of persimmon plant (Diospyros kaki) for physiological benefits and bioaccessibility of antioxidants by in vitro simulated gastrointestinal digestion. Food Chem. 2017;214:478-85.
21. Mohsin S, Akhtar N. Formulation and stability evaluation of bauhinia variegata extract topical emulsion. Acta Pol Pharm. 2017;74(3):945-54.
22. Akhtar N, Akhtar N, Menaa F, Alharbi W, Alaryani FSS, Alqahtani AM, et al. Fabrication of ethosomes containing tocopherol acetate to enhance transdermal permeation: In vitro and ex vivo characterizations. Gels. 2022;8(6):335.
23. Ijaz M, Akhtar NJJocd. Fatty acids based α‐Tocopherol loaded nanostructured lipid carrier gel: In vitro and in vivo evaluation for moisturizing and anti‐aging effects. 2020;19(11):3067-76.
24. Panwar A, Upadhyay N, Bairagi M, Gujar S, Darwhekar G, Jain D. Emulgel: A review. Asian J Pharm Life Sci. 2011;2231:4423.
25. Kim B, Choi J, Park K, Youn S. Sebum, acne, skin elasticity, and gender difference–which is the major influencing factor for facial pores? Skin Res Technol. 2013;19(1):e45-e53.
26. Varma VNSK, Maheshwari P, Navya M, Reddy SC, Shivakumar H, Gowda D. Calcipotriol delivery into the skin as emulgel for effective permeation. Saudi Pharmaceutical Journal. 2014;22(6):591-9.

Most read articles by the same author(s)

<< < 1 2 3 > >>