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Ajay Kumar Yadav
Kapil Malviya
Pushpendra Soni
Pushpendra Kumar
Lavakesh Kumar Omray


Metronidazole, chitosan, pluronic, hydrogel, controlled release


The objective of the present investigation was to explore the hydrogel microspheres loaded with metronidazole to obtain controlled release for ocular delivery. The FTIR spectra of pure drug and the drug excipient mixture revealed no chemical interaction. Metronidazole loaded hydrogel microspheres were prepared using Chitosan/Pluronic F68 blend and emulsion crosslinking method. MHM7 with blend ratio 3.33:1 exhibited the highest encapsulation efficiency (77.1505%) whereas the lowest encapsulation was witnessed in the formulation MHM3 (34.71%) which has a blend ratio of 15:1. The particle size ranged from 26.96 µm for MHM3 to 134.8 µm for MHM7. The microspheres exhibited negative zeta potential and the value was found to be -18.1 mV for MHM7. MHM3 exhibited the highest water uptake (350.5 %) whereas MHM7 exhibited the lowest water uptake (117.5 %).  MHM7 released around 59.54% drug after 24 h. Formulation MHM4 was also able to control the release to a great extent with 67.37% drug released at the end of 24 h but it exhibited a lower encapsulation efficiency of 61.771%. Hence MHM7 was considered to be the most optimized formulation. The release of metronidazole from MHM7 followed Higuchi mathematical model suggesting Non-Fickian diffusion.

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1.; assessed on 13/07/2023
2. Kyung JH, Yeon KS, Jeon, KS, Moo LY. pH/temperature-responsive semi­IPN hydrogels composed of alginate and poly(N-isopropylacrylamide). J Appl. Polym. Sci. 2002; 83: 128.
3. Qu X, Wirse 'n A, Albertsson AE. Novel pH-sensitive chitosan hydrogels: swelling behavior and states of water. Polymer. 41 (2000) 4589.
4. Patil-Vibhute PB, Hajare AA. Preparation and characterization of superporous hydrogels as gastroretentive drug delivery system for atenolol. International Journal of Pharmaceutical Sciences and Research. 2019; 10(1): 272-285. DOI: 10.13040/IJPSR.0975-8232.10(1).272-85.
5. Rajini Kanth V, Kajjari PB, Madalageri PM, Ravindra S, Manjeshwar LS, Aminabhavi TM, Vallabhapurapu VS. Blend Hydrogel Microspheres of Carboxymethyl Chitosan and Gelatin for the Controlled Release of 5-Fluorouracil. Pharmaceutics. 2017, 9: 13. DOI: 10.3390/pharmaceutics9020013.
6. Shahid F, Aman A, Nawaz MA, Karim A, Ul Qader SA. Chitosan hydrogel microspheres: an effective covalent matrix for crosslinking of soluble dextranase to increase stability and recycling efficiency. Bioprocess and Biosystems Engineering. 2016. DOI 10.1007/s00449-016-1713-7.
7. Mallikarjuna B, Madhusudana Rao K, Sudhakar P, Chowdoji Rao K, Subha MCS. Chitosan Based Biodegradable Hydrogel Microspheres for Controlled Release of an Anti HIV Drug. Indian Journal of Advances in Chemical Science. 2013; 1(3): 144-151.
8. Rokhade AP, Shelke NB, Patil SA, Aminabhavi TM. Novel hydrogel microspheres of chitosan and pluronic F-127 for controlled release of 5-fluorouracil. Journal of Microencapsulation. 2007; 24(3): 274–288. DOI: 10.1080/02652040701281365.
9. Naveed S, Qamar F. Simple UV Spectrophotometric Assay of Metronidazole. Open Access Library Journal. 2014; 1: e615
10. Rokhade AP, Agnihotri SA, Patil SA, Mallikarjuna NN, Kulkarni PV, Aminabhavi TM. Semi-interpenetrating polymer network microspheres of gelatin and sodium carboxymethyl cellulose for controlled release of ketorolac tromethamine. Carbohydrate Polymers. 2006; 65: 243–252.
11. Gu WZ, Hu XF. Pluronic F127/chitosan blend microspheres for mucoadhesive drug delivery. IOP Conference Series: Materials Science and Engineering. 2017; 167: 012001.
12. Gunbas ID, Sezer UA, Iz SG, Gürhan IDm, Hasirci N. Semi-IPN chitosan/PEG microspheres and films for biomedical applications: characterization and sustained release optimization. Industrial & Engineering Chemistry Research. 2012; 51: 11946-11954.