PHYTOCHEMICAL QUANTIFICATION, ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL OF SWERTIA CHIRAYITA FROM PAKISTAN

Main Article Content

Lubna Sartaj
Saira Farman
Zahida Parveen
Asma Waheed Qureshi
Beenish Khurshid
Nosheen Faiz
Soofia Iftikhar
Syed Bilal Shah

Keywords

Swertia chirayita, phytochemicals, antimicrobial activity, antioxidant property

Abstract

The present study was carried out to determine various phytochemicals from Swertia chirayita and to evaluate antimicrobial and antioxidant properties of the extracted phytochemicals. Phytochemical determination i.e., flavonoids, tannins, β-carotene and lycopene, and alkaloids was carried out using different methods. Among the phytochemicals, flavonoids i.e., 67.4 mg/ml were present in higher concentrations, tannins i.e., 38.2 mg/ml in moderate concentrations, while β-carotene i.e., 0.678 mg/50 ml, lycopene i.e., 0.150 mg/50 ml and alkaloids i.e., 0.073 mg were found in trace amounts. Among various extracts, acetone extract had shown antimicrobial activity against Klebsiella pneumonia, Haemophilus influenza, Acinetobacter baumanii and Morganella morganii, while it was inactive against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Ethanol extract showed activity only against Haemophilus influenza and all other microorganisms showed resistance against ethanol extract. Other all the tested extracts showed no notable activity against the microorganisms. Flavonoid and tannin showed significant antioxidant activity and among the extracts ethanol and methanol showed higher antioxidant activity, n-hexane, acetone and chloroform showed moderate activity followed by butanol and water showing the lowest activity. Thus, S. chirayita possesses significant antimicrobial and antioxidant properties and can be used to treat pathogenic infections.

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References

1. Alam KD, A. M. (2010). Report: analgesic activities of ethanol extract of leaf, stem and their different fractions of Swertia chirata. Pakistan Journal of Pharmaceutical Sciences, 4(23), 455-457.
2. Ferreira, L. B. (2007). Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. European Food Research and Technology, 225(2), 151-156.
3. Goutam Brahmachari, S. M. (2004). Swertia (Gentianaceae): Chemical and Pharmacological Aspects. CHEMISTRY & BIODIVERSITY, 1(11), 1627–1651.
4. Goutam Brahmachari, S. M. (2004). Swertia (Gentianaceae): Chemical and Pharmacological Aspects. CHEMISTRY AND BIODIVERSITY, 1(11), 1627–1651.
5. Gow-Chin Yen, H.-Y. C. (1995). Antioxidant Activity of Various Tea Extracts in Relation to Their Antimutagenicity. JOURNAL OF AGRICULTURE AND FOOD CHEMISTRY, 43(1), 27–32.
6. H O Edeoga, D. E. (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, 4(7), 685-688.
7. Harinder P S Makkar, M. B. (1993). Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. Science of Food and Agriculture, 61(2), 161-165.
8. In-Kyoung Lee, S.-J. S.-K.-S. (2006). Hispidin Derivatives from the Mushroom Inonotus xeranticus and Their Antioxidant Activity. JOURNAL OF NATURAL PRODUCTS, 69 (2), 299–301.
9. Joshi, K. (2008). Swertia L. (Gentianaceae) in Nepal: Ethnobotany and Agenda for Sustainable Management. Ethnobotanical Leaflets, 2008(1).
10. Joshi, K. (2008). Swertia L. (Gentianaceae) in Nepal: Ethnobotany and Agenda for Sustainable Management. Ethnobotanical Leaflets(1).
11. Joshi, K. (2008). Swertia L. (Gentianaceae) in Nepal: Ethnobotany and Agenda for Sustainable Management. Ethnobotanical Leaflets, 2008(1).
12. M. Kaneria, Y. B. (2009). Determination of Antibacterial and Antioxidant Potential of Some Medicinal Plants from Saurashtra Region, India. Indian Journal of Pharmaceutical Sciences, 71(4), 406–412.
13. M.P.Revuelta, B. (1997). Depolarization-dependent effect of flavonoids in rat uterine smooth muscle contraction elicited by CaCl2. General Pharmacology: The Vascular System, 29( 5), 847-857.
14. Michael Keil, B. H. (2000). Production of Amarogentin in Root Cultures of Swertia chirata. Thieme, 5(66), 452-457.
15. Michael Keil, B. H. (2000). Production of Amarogentin in Root Cultures of Swertia chirata. Thieme, 5(66), 452-457.
16. Nethravathi Guthalu Puttaraju, S. U. (2006). Antioxidant Activity of Indigenous Edible Mushrooms. JOURNAL OF AGRICULTURE AND FOOD CHEMISTRY, 54(26), 9764–9772.
17. PaoloScartezzini, E. (2000). Review on some plants of Indian traditional medicine with antioxidant activity. Journal of Ethnopharmacology, 71(1-2), 23-43.
18. Satyendra Suryawanshi, R. A. (2007). Simultaneous estimation of mangiferin and four secoiridoid glycosides in rat plasma using liquid chromatography tandem mass spectrometry and its application to pharmacokinetic study of herbal preparation. Journal of Chromatography B, 858(1-2), 211-219.
19. SHIVAJI BANERJEE, T. K. (2000). ASSESSMENT OF THE ANTI-INFLAMMATORY EFFECTS OF SWERTIA CHIRATA IN. Indian Journal of Pharmacology(32), 21-24.
20. Susanna Phoboo, P. K. (2010). Trade and Sustainable Conservation of Swertia chirayita (Roxb. ex Fleming) h. Karst in Nepal. Nepal Journal of Science and Technology, 11, 125-132.
21. Timothy Johns, P. B. (2006). Linking biodiversity, diet and health in policy and practice. Proceedings of the Nutrition Society, 65(2), 182-189.
22. Timothy Johns, P. B. (2006). Linking biodiversity, diet and health in policy and practice. Proceedings of the Nutrition Society, 65(2), 182-189.
23. Victor B.Owoyele, Y. Y. (2005). Analgesic and anti-inflammatory properties of Nelsonia canescens leaf extract. Journal of Ethnopharmacology, 99(1), 153-156.

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