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Raazia Waseem
Ayesha Asif
Komal Najam
Usman Minhas
Roha Asif
Muhammad Maaz Arif
Ayesha Seerat
Hajra Sohail
Maria Akhtar


Traditional use, Cannabis sativa, Ficus carica, Dodonea viscosa, Phytochemical composition, Antibacterial activit


The traditional use of Cannabis sativa, Ficus carica and Dodonea viscosa in local medications to offset the bacterial infections. This study analysed phytochemical composition of medicinal plants leaves extracts in aqueous, acetone and methanol solvent. Antibacterial activity of extracts was assayed against 6 bacterial species Staphylococcus aureus, Bacillus cereus, Bacillus pumilus, Streptococcus spp., Salmonella typhi and Acetobacter spp. through the disk diffusion method. Antibacterial potential of extracts is explainable on bases of phytochemical composition as ethanolic extract strongly inhibits bacterial growth. Minimum inhibitory concentration (MIC) performed for ethanolic extract of Cannabis sativa at various concentrations of dilutions. In vitro antiproliferative potential was determined by MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] on HeLa cell lines. Confluent monolayers of cells were used to test the cytotoxicity ethanolic extracts. ELISA reader calculated optical densities at 570 nm to measure the cell survival percentage and EC50.  A dose dependent curve was obtained by plotting concentration of dose and cell viability. These findings promote the discovery of active compounds that might serve as lead molecules in the creation of novel antibacterial and antiproliferative medications.

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