Molecular study of human metapneumovirus among children under 5 years with acute respiratory tract infection in Basrah city, Iraq

Main Article Content

Noora Khudhur Abdulqader
Hazim Talib Thwiny
Aida A. Manthar

Keywords

Factors, Infections, Work, Results

Abstract

Human metapneumovirus (hMPV) is a major global factors that contributes to children getting acute respiratory tract infections (ARTI). This virus is frequently present in paediatric patients, with children under the age of two being particularly vulnerable. However,
they are very common from early childhood until age five. Two hundred oropharyngeal swabs were collected from children with respiratory tract infections (RTIs), between December 2020 to December 2021 and were tested for hMPV. The database registered in the work included the sex, age, the season in which the specimen was obtained, feeding style, and the mother's education of infected children as well as major clinical respiratory tract infection symptoms. A molecular method was used to identify the presence of hMPV, and the results showed that 13% of the 200 samples tested positive for the virus. The samples were further broken down based on age groups, sex, season, feeding style, and mothers' education. According to age, the infection rate was noticed high in the age group (2-24 months) which was (13.7%) compared with (25-60 months) age group (12.4%) and there is no significant difference. The results were also shown according to the sex distribution, the infection with hMPV was more in males (14.8%) rather than females (9.7%). But this difference was not significant (P > 0.05). The majority of cases of hMPV occur in the winter and spring with a rate (15.8%), followed
by (11.1%) in autumn. While 0 (0%) in summer. There was no noticeable difference (P > 0.05). The majority of hMPV infections have been identified with a rate (18.2%) in mixed feeding, followed by (13.0%) in bottle feeding and (10%) in breastfeeding. There was no significant difference (P > 0.05). while the distribution of infection hMPV against mother’s education was as follows: 13.2% in 7-12
years and 12.9% in ≤6 years and 12.8% in ≥ 13 years.

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References

1. Al-bahadily, A. K. J. M., Al-Omrani, A. A. A. M., & Atiya, A. A. (2017). Hypertonic 3% saline in comparison with 0.9%(normal) saline in the
treatment of acute bronchiolitis. International Journal of Pediatrics, 5(1), 4209-4216.
2. Al-Hisnawi, H. T., & Abood, W. S. (2022). Molecular detection of Human metapneumovirus in children with RTI in Al-najaf city.
3. Almayahi, W. M., Saeed Abbas, S., & Abbas, B. A. (2022). Molecular Detection and Histopathological Effect of Infectious Bronchitis
Virus Circulating in Vaccinated Broiler Flocks in Basrah, Iraq. Iranian Journal of War and Public Health, 14(3), 339-345.
4. Alsuheel, A. M., Ali, A. S., Al-Hakami, A. M., Shati, A. A., Chandramoorthy, H. C., & AlQahtani, S. M. (2019). Human metapneumovirus
in pediatric patients with acute respiratory tract infections in the Aseer region of Saudi Arabia. Saudi Journal of Medicine & Medical
Sciences, 7(2), 80.
5. Boivin, G., Abed, Y., Pelletier, G., Ruel, L., Moisan, D., Côté, S., ... & Anderson, L. J. (2002). Virological features and clinical manifestations
associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratorytract infections in all age groups. The Journal of infectious diseases, 186(9), 1330-1334.
6. Boivin, G., De Serres, G., Côté, S., Gilca, R.,Abed, Y., Rochette, L., ... & Déry, P. (2003). Human metapneumovirus infections in
hospitalized children. Emerging infectious diseases, 9(6), 634.
7. Divarathna, M. V. M., Rafeek, R. A. M., Morel, A. J., Jagoda, S., & Noordeen, F. (2021). Seasonal distribution and phylogenetic analysis of human metapneumovirus in children with acute respiratory tract infections admitted to a general hospital in Sri Lanka. Sri Lankan Journal of
Infectious Diseases, 11.
8. Esper, F., Boucher, D., Weibel, C., Martinello, R. A., & Kahn, J. S. (2003). Human metapneumovirus infection in the United States:
clinical manifestations associated with a newly emerging respiratory infection in children. Pediatrics, 111(6), 1407-1410.
9. Etiler, N., Velipasaoglu, S., & Aktekin, M. (2002). Incidence of acute respiratory infections and the relationship with some factors in infancy
in Antalya, Turkey. Pediatrics international, 44(1), 64-69.
10. Fouquet, C.; Mayo, M.; Maniloff, J.;Desselberger, U. (2006). Virus taxonomy: classification and nomenclature of viruses. Eighth
Report of the International Committee on Taxonomy of Viruses. Elsevier/Academic Press, Oxford, United Kingdom.11. Freymuth, F., Vabret, A., Legrand, L.,Eterradossi, N., Lafay-Delaire, F., Brouard, J., &Guillois, B. (2003). Presence of the new human metapneumovirus in French children with bronchiolitis. The Pediatric infectious disease journal, 22(1), 92-94.
12. Gálvez, N., Andrade, C. A., Pacheco, G. A., Soto, J. A., Stranger, V., Rivera, T., ... & Kalergis, A. M. (2021). Host Components That Modulate the
Disease Caused by hMPV. Viruses, 13(3), 519.
13. Garcia, J., Sovero, M., Kochel, T., LagunaTorres, V. A., Gamero, M. E., Gomez, J., ... & Halsey, E. S. (2012). Human metapneumovirus
strains circulating in Latin America. Archives of Virology, 157, 563-568.
14. Hall, W. B., Kidd, J. M., Campbell-Bright, S.,Miller, M., &Aris, R. M. (2011). Clinical manifestations and impact of human
metapneumovirus in healthy adults: A retrospective analysis of 28 patients over 2 years. In C59. H1N1 INFLUENZA AND OTHER
VIRAL INFECTIONS: CLINICAL MANIFESTATIONS, DIAGNOSIS, AND OUTCOMES (pp. A4927-A4927). American Thoracic Societ.
15. Hameed, H. G., & Jubair, R. S. (2021). Parents’ Knowledge and Attitudes toward Seasonal Childhood Influenza Vaccination for Children
Aged below 5 Years in Hilla City/Iraq. Medical Journal of Babylon, 18(2), 126.
16. Hassan, D. A. (2015). Characterization of human coronaviruses from children with respiratory infection in Erbil Kurdistan region of
Iraq (Doctoral dissertation, HAWLER MEDICAL UNIVERSITY).
17. Hassan, D., Rachid, S. and Ziebuhr, J. (2018). A Single-Center Study of Viral Respiratory Tract Infections in Hospitalized Children from the
Kurdistan Region of Iraq. Global Pediatric Health, 5, pp.2333794X1878499.
18. Hassan, M. A. K., & Al-Sadoon, I. (2001). Risk factors for severe pneumonia in children in Basrah. Tropical doctor, 31(3), 139-141.
19. Heikkinen, T., & Järvinen, A. (2003). The common cold. The Lancet, 361(9351), 51-59.
20. Ji, L., Chen, L., Xu, D., & Wu, X. (2021). Molecular typing and epidemiologic profiles of human metapneumovirus infection among
children with severe acute respiratory infection in Huzhou, China. Molecular Biology Reports, 48(12), 7697-7702.
21. Kadhim Al-Jaferi, N. (2014). Nutritional risk factors for acute lower respiratory tract infection among infants and children 2- 60 months old in Basra, Southern Iraq. The Medical Journal of Basrah University, 32(1), 30-36.
22. Kaida, A., Iritani, N., Kubo, H., Shiomi, M., Kohdera, U., & Murakami, T. (2006). Seasonal distribution and phylogenetic analysis of human
metapneumovirus among children in Osaka City, Japan. Journal of Clinical Virology, 35(4), 394- 399.
23. Lanari, M., Prinelli, F., Adorni, F., Di Santo, S., Faldella, G., Silvestri, M., &Musicco, M. (2013). Maternal milk protects infants against
bronchiolitis during the first year of life. Results from an Italian cohort of newborns. Early human development, 89, S51-S57.
24. Mackay, I. M., Arden, K. E., & Nitsche, A. (2002). Real-time PCR in virology. Nucleic acids research, 30(6), 1292-1305.
25. Mahony, J. B., Petrich, A., & Smieja, M. (2011). Molecular diagnosis of respiratory virus infections. Critical reviews in clinical laboratory
sciences, 48(5-6), 217-249.
26. Moattari, A., Aleyasin, S., Arabpour, M. and Sadeghi, S., (2010). Prevalence of human Metapneumovirus (hMPV) in children with
wheezing in Shiraz-Iran. Iranian Journal of Allergy, Asthma and Immunology, pp.251-254.
27. Peiris, J. S., Tang, W. H., Chan, K. H., Khong, P. L., Guan, Y., Lau, Y. L., & Chiu, S. S. (2003). Children with respiratory disease associated with
metapneumovirus in Hong Kong.
28. Peret, T. C., Boivin, G., Li, Y., Couillard, M., Humphrey, C., Osterhaus, A. D., ... & Anderson, L. J. (2002). Characterization of human
metapneumoviruses isolated from patients in North America. Journal of Infectious Diseases, 185(11), 1660-1663.
29. Ramaekers, K., Keyaerts, E., Rector, A., Borremans, A., Beuselinck, K., Lagrou, K., & Van Ranst, M. (2017). Prevalence and seasonality
of six respiratory viruses during five consecutive epidemic seasons in Belgium. Journal of Clinical Virology, 94, 72-78.
30. Salem, M. B., Al Sadoon, I. O., & Hassan, M. K. (2002). Prevalence of wheeze among preschool children in Basra governonate, southern
Iraq. EMHJ-Eastern Mediterranean Health Journal, 8 (4-5), 503-508, 2002.
31. Tregoning, J. S., & Schwarze, J. (2010).Respiratory viral infections in infants: causes, clinical symptoms, virology, and
immunology. Clinical microbiology reviews, 23(1), 74-98.
32. Van den Hoogen, B. G., van Doornum, G. J., Fockens, J. C., Cornelissen, J. J., Beyer, W. E., Groot, R. D., ... & Fouchier, R. A. (2003).
Prevalence and clinical symptoms of human metapneumovirus infection in hospitalized patients. The Journal of infectious diseases,
188(10), 1571-1577.
33. Zhang, S. F., Tuo, J. L., Huang, X. B., Zhu, X., Zhang, D. M., Zhou, K., ... & Xu, L. (2018). Epidemiology characteristics of human
coronaviruses in patients with respiratory infection symptoms and phylogenetic analysis ofHCoV-OC43 during 2010-2015 in
Guangzhou. Plos one, 13(1), e0191789.
34. Zhou, J. Y., Peng, Y., Peng, X. Y., Gao, H. C., Sun, Y. P., Xie, L. Y., ... & Cao, Y. D. (2018). Human bocavirus and human metapneumovirus
in hospitalized children with lower respiratory tract illness in Changsha, China. Influenza and Other Respiratory Viruses, 12(2), 279-286.