DESIGN, OPTIMIZATION AND IN-VITRO EVALUATION OF URARIA PICTA EXTRACT LOADED SILVER NANOPARTICLES FOR CONTROLLED DRUG RELEASE
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Keywords
AgNO, Nanoparticles, Extract , Uraria picta, Particle size, UV–Visible spectroscopy
Abstract
The Plant based nanoparticles synthesis is a new technique where biological molecules and their combinations in plant aqueous extract decrease metal salts, which have a dual role in the fabrication and stabilization of metallic NPs. Aim: The present study aimed to develop and optimize green synthesized silver nanoparticles (AgNPs) using Uraria picta (UPAgNps) plant extract through an eco-friendly and cost-effective method. Materials and Methods: Silver nanoparticles were synthesized via plant-mediated reduction using aqueous silver nitrate (1–3 mM) and varying concentrations of Uraria picta extract (50–250 mg/mL). Optimization was performed using a Box–Behnken Design (BBD) considering AgNO₃ concentration, extract concentration, and stirring time as independent variables. Particle size and zeta potential were selected as response parameters. The optimized formulation was characterized by UV–Visible spectroscopy, particle size analysis, zeta potential measurement, scanning electron microscopy (SEM), entrapment efficiency, and in vitro drug release studies using the dialysis membrane method. Drug release kinetics were evaluated using Zero-order, First-order, Higuchi, and Korsmeyer–Peppas models. Results: The optimized formulation showed significant model validity with a Model F-value of 85.01 (p < 0.05). Extract concentration and AgNO₃ concentration significantly influenced particle size and zeta potential. The optimized nanoparticles exhibited desirable particle size with good stability and a zeta potential indicating sufficient electrostatic stabilization. The entrapment efficiency was found to be 88.45%. SEM analysis confirmed nanoscale morphology. In vitro drug release demonstrated sustained release up to 12 hours, and kinetic modeling suggested diffusion-controlled release behavior following Higuchi and Korsmeyer–Peppas models. Conclusion: The study successfully formulated optimized Uraria picta extract-mediated silver nanoparticles using a green synthesis approach. The developed nanoparticles showed high entrapment efficiency, controlled drug release, and good stability, indicating their potential as an effective and eco-friendly nanocarrier system for improved drug delivery applications.
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Dr. Asha Verma
Professor, Department of chemistry , Govt. Dr. Shyama Prasad Mukherjee science and commerce college Kolar, Bhopal (Madhya Pradesh)
Mahesh Kumar Ahirwar
Research scholar , Department of chemistry, Govt. Dr. Shyama Prasad Mukherjee science and commerce college Kolar, Bhopal (Madhya Pradesh)
