ANTIBIOTIC SUSCEPTIBILITY PATTERN OF PSEUDOMONAS AERUGINOSA ISOLATED FROM NOSOCOMIAL INFECTIONS IN A TERTIARY CARE HOSPITAL
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Abstract
INTRODUTION: Pseudomonas aeruginosa is an opportunistic, non-fermenting Gram-negative bacillus that has become a leading cause of healthcare-associated (nosocomial) infections worldwide, especially in critically ill and immunocompromised patients. the present study was designed to evaluate the antibiotic susceptibility pattern of P. aeruginosa isolated from nosocomial infections in a tertiary care hospital.
METHODS: This cross-sectional study was done in the Department of Microbiology, Index Medical College, Hospital and Research Centre, M.U. Indore MP. 300 samples from patients of all age group and both sexes were enrolled in the study. All isolates of Pseudomonas aeruginosa from a variety of clinical specimens, including pus, urine, burn wounds, sputum, bodily fluids, CSF, tracheal secretions, and patient devices were included in the study. The isolates were identified by colony morphology, Gram’s staining and biochemical test according to standard laboratory test methods. In accordance with CLSI 2025 guidelines, each isolate was subjected to an AST using the Kirby-Bauer disc diffusion method.
RESULTS: Out of 300 patients, 225 (75%) were male and 75 (25%) were female. Most commonly affected age group was 46-60 years (30%), followed by 61-75years (24.7%). Pseudomonas aeruginosa was most commonly isolated from pus samples (39.7%) followed by sputum sample (32.3%), urine sample (10.3%). Pseudomonas aeruginosa isolates were 100% susceptible to colistin and polymyxin B. 94.7% isolates were susceptible to imipenem and meropenem. 87.3% isolates were susceptible to piperacillin tazobactam. 81.7% isolates were susceptible to amikacin. 75.7% isolates were susceptible to ciprofloxacin. 73.7% isolates were susceptible to gentamicin. 70.3% isolates were susceptible to netilmicin. 63.3% isolates were susceptible to carbenicillin. 52% isolates were susceptible to aztreonam. Only 33.4% isolates were susceptible to ceftazidime.
CONCLUSION: The study provides updated, hospital-specific antibiotic susceptibility data that can assist clinicians in selecting appropriate empirical and targeted therapy for Pseudomonas aeruginosa infections.
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Surbhi Verma
PhD. Scholar, Department of Microbiology, Index Medical college hospital & research centre, Indore, Madhya Pradesh.
Dr Kailash Jatav
Associate professor, Department of Microbiology ,Index Medical college hospital & research centre, Indore, Madhya Pradesh.
Aneesh Sharma
PhD. Scholar, Department of Microbiology, Index Medical college hospital & research centre, Indore, Madhya Pradesh.
