IDENTIFICATION OF NOVEL BIOACTIVE NATURAL COMPOUNDS AND THEIR BIOLOGICAL ACTIVITIES IN N-HEXANE EXTRACTS OF CARICA PAPAYA VARIETY OF RED LADY LEAVES WITH GAS-CHROMATOGRAPHY-MASS SPECTROMETRY
Main Article Content
Keywords
Carica Papaya, Gas Chromatography-Mass Spectrometry, Phytocomponents, n-hexane
Abstract
Objective: The present study was to determine of novel bioactive natural compounds and their biological activities in n-hexane extract of Carica Papaya variety of Red Lady Leaves.
Methods: Fresh green leaves of the C. P variety of Red Lady were used to make extract with n-hexane. Then this extract was subjected to a phyto-chemical compounds analysis utilizing an Agilent Technologies (7890-A-7000) GC-MS Triple-Quad. The Automated Mass Spectral Deconvolution and Identification System sophisticated software for GC/MS data interpretation from the National Institute of Standards and Technology, was utilized to generate a Target Component Library file.
Results: GC/MS assay used Retention Time, Peak Area, and Molecular Weight, revealing the presence of different bio-active components, an over-all of fifty-two components were detected as distinctive to be frequent in n-hexane-extract. However, when analyzing the relative abundance of phyto-components, occurring in C. P Red Lady Leaves (n-hexane extract), the phyto-components and their relative abundance were 1,2-Benzene-dicarboxylic Acid, mono 2 ethyl hexyl Ester 47.58%, 9,12,15-Octa-decatrienoic Acid, Ethyl Ester, (Z, Z ,Z)- 7.29%, and Retinoic Acid, 5,6-epoxy-5,6-di hydro- 4.07%. GC/MS analysis of the n-hexane extract recognized various phyto-chemical compounds, with a mean Peak Area % of 1.94 ± 6.77 (range: 0.06–47.58) and a mean Target Component Library file similarity of 82.22 ± 8.94 (range: 46.70–94.60). One-sample t-test demonstrated that the mean Peak Aera % was significantly <5% benchmark (t = –3.17, p = 0.0027), showing that most compounds were present in trace abundance.
Conclusion: The GC-MS reporting of Carica Papaya variety of Red Lady Leaves (n-hexane extract) unveiled the occurrence of numerous bio-active constituents as well as significant therapeutic properties. Therefore, the presence of these phyto-chemicals may exist responsible for the medicinal effects.
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Asadullah
Lecturer, Department of Pharmacology, Shaheed Mohtarma Benazir Bhutto Medical College, Karachi, Pakistan.
Asif Ahmed
Professor, Department of Pharmacology & Therapeutics, Baqai Medical College, Baqai University, Karachi, Pakistan.
Sobia Parveen
Assistant Professor, Department of Biochemistry, Rai Medical College, Sargodha, Pakistan.
Fauzia Jan
Assistant Professor, Department of Biochemistry, University Medical & Dental College, Faisalabad, Pakistan.
Sheikh Maria Qammar
Associate Professor, Department of Pharmacology, Rashid Latif Medical College, Lahore, Pakistan.
Sidra Anwar
Department of Biochemistry, Independent Medical College, Faisalabad, Pakistan.
