COMPARATIVE ASSESSMENT OF CITRUS LIMETTA WITH IMIPRAMINE AND DIAZEPAM FOR DEPRESSION AND ANXIET
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Keywords
Citrus limetta, Depression,Anxiety, CNS depressant,Immobility.
Abstract
This study examines the antidepressant and anxiolytic effects of the ethanolic extract of Citrus limetta (EELC) in Swiss albino mice. The extract was obtained through the Soxhlet extraction technique and given orally at doses of 100, 200, and 300 mg/kg for a period of 14 days. The study was divided into different groups: a control group that received saline, a standard antidepressant group treated with imipramine (15 mg/kg), and a standard anxiolytic group treated with diazepam (1 mg/kg). To evaluate antidepressant activity, the forced swim test (FST) and tail suspension test (TST) were used. Meanwhile, the light–dark box, head dip, and marble burying tests were conducted to assess the anxiolytic effects. The findings revealed that oral administration of EELC led to a dose-dependent decrease in immobility time during both the FST and TST, with higher doses showing effects similar to imipramine. In the anxiety models, animals treated with EELC spent more time in the light compartment, performed a greater number of head dips, and showed a marked reduction in marble burying behavior, indicating significant anxiolytic activity. The results highlight Citrus limetta's potential as a natural alternative for the treatment of mood and anxiety disorders by showing that its ethanolic extract has both antidepressant and anxiolytic properties in mice.
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Darshna kumari
Faculty of Pharmacy and Pharmaceutical Sciences. University of Karachi.
Syeda Afroz
Faculty of Pharmacy and Pharmaceutical Sciences. University of Karachi.
Joshna kumari
Liaquat University of Medical and Health Sciences Jamshoro .Pakistan
Saeeda Naseer
Basic Medical Sciences Institute (BMSI), JPMC, Karachi.
Abdul Waheed Solangi
Basic Medical Sciences Institute (BMSI), JPMC, Karachi.
Iqra Rana
Faculty of Pharmacy and Pharmaceutical Sciences. University of Karachi.
Sajjad Ali
Faculty of Pharmacy and Pharmaceutical Sciences. University of Karachi.
Muqeet Kazim
Faculty of Pharmacy and Pharmaceutical Sciences. University of Karachi.
Syeda Noor
Faculty of Pharmacy and Pharmaceutical Sciences. University of Karachi.
Tahmina Abid
Faculty of Pharmacy and Pharmaceutical Sciences. University of Karachi.
Maira Iqbal
Faculty of Pharmacy and Pharmaceutical Sciences. University of Karachi.