ENDOCRINE FACTORS ASSOCIATED WITH SUCCESSFUL IN VITRO FERTILIZATION PREGNANCY
Main Article Content
Keywords
AMH; FSH; TSH; LH; Estrogen; Progesterone; Prolactin; In vitro fertilization.
Abstract
Background: Infertility is the term used when a clinical pregnancy cannot be achieved within at least 12 months. Thyroid dysfunction impacts the anti-Mullerian hormone (AMH), a recognized marker of ovarian function. Proper growth, sexual maturation, and reproductive processes rely on gonadotropins, peptide hormones that regulate ovarian and testicular functions.
Objective: The objective of this study is to assess the serum levels of specific endocrine factors, including anti-mullerian hormone, prolactin, gonadotropins, sex-related hormones, and thyroid-stimulating hormones in women undergoing IVF treatment.
Materials and Methods: The study consisted of women who underwent IVF at the Australian Concept Infertility Medical Center, Lahore. This was a cross-sectional study involving 80 women undergoing IVF. The causes of infertility were distributed as follows: female infertility, primary and secondary, tubal factor infertility, polycystic ovary syndrome, endometriosis, and other reasons. Blood samples were collected from patients undergoing IVF on various days to analyze endocrine variables. Enzyme-linked immunosorbent assay (ELISA) kits were used to detect FSH, LH, estrogen, progesterone, AMH, TSH, and prolactin. Statistical analysis was conducted using SPSS.
Results: Mean age of women was 33.42±4.74 years. Among women 76(95%) were nulliparous and only 3(3.8%) women were prim-parous while only 1(1.3%) women present with parity 4. Mean body mass index of women was 27.20±4.68. 64(80%) women were present with primary and 16(20%) presents with secondary infertility. The most frequent blood group among women was O+ve (57.5%), followed by B+ve (31.25%), AB+ve (6.25%), and A+ve (5%), respectively. Mean FSH level in women was 6.36±2.92. Mean LH level in women was 5.24±2.63. Mean prolactin level in women was 19.71±12.33. Mean serum AMH level among women was 2.49±1.68. The mean AMH level ranges between 0.09 and 19.30. Mean TSH level among women was 2.02±1.29. Mean estrogen and progesterone levels among women were 108.70±80.44 and 2.98±2.19, respectively.
Conclusion: Our findings demonstrated that FSH, LH, prolactin, AMH, and estrogen levels were slightly higher among women with positive pregnancy outcomes. Women with negative pregnancy outcomes had higher values for serum TSH and progesterone levels.
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Rihana Dilshad
Assistant Professor at Sheikh Zayed Medical College, Rahim Yar Khan, Pakistan.
Hina Allah Ditta
Additional Principal Women Medical Officer at Sheikh Zayed Medical College, Rahim Yar Khan, Pakistan.
Madiha Shahzad Lodhi
Assistant Professor at the University of Lahore, Pakistan.
Rizwana Dilshad
Associate Professor at Swedish College of Pharmacy and Allied Health Sciences, Rahim Yar Khan, Pakistan.
Maqsood Ahmad
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Rabia Nisar
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Pakistan
Aqsa Afzal
Faculty of Pharmacy, Department of Pharmaceutics, Bahauddin Zakaria University, Multan, Pakistan.
Sultan Ahmad
Assistant Professor, Thoracic surgery, Sheikh Zayed Medical College and Hospital, Rahim Yar Khan, Pakistan.