ANALYSIS AND ASSOCIATION OF POLYMORPHISM IN TLR-3 GENE WITH LIVER COMPLICATIONS IN CHRONIC HCV PAKISTANI PATIENTS
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Keywords
TLR-3 gene, allele polymorphism, HCV infection, susceptibility, resistance, fibrosis
Abstract
Hepatitis C virus (HCV) causes a significant health challenge in Pakistan. Previous research has suggested a correlation between polymorphisms in the TLR-3 gene and HCV infection. This study is aimed to analyze TLR-3 gene polymorphism in Pakistani individuals with chronic HCV infection and their association with liver complications. The study analyzes the exon-4 region of the TLR-3 gene through sequencing and clinical parameters in 110 chronically HCV-infected patients with various liver complications. A total of 120 healthy subjects are also included for comparison of TLR-3 polymorphism. The distribution of the TLR-3 SNP rs3775290 genotypes as CC (48.7%), CT (40.9%), and TT (10.4%), demonstrates a statistically significant association in both genotype and allele frequencies within the study population. Notably, the CC genotype is more frequently observed in a comparative healthy group (63%), while the CT genotype is predominant in HCV-infected individuals (56%), and the TT genotype is most prevalent in patients with liver fibrosis (43%). Genotype distributions for both SNPs were consistent with Hardy-Weinberg expectations. Furthermore, the study found that higher HCV viral loads and more advanced stages of liver fibrosis correlated with specific rs3775290 genotypes.
It is concluded that the CC genotype of TLR-3 rs3775290 is prevalent and associated with a potential protective effect against chronic HCV infection in the study population. Moreover, the CT genotype appears associated to HCV chronicity, and the TT genotype with the progression to advanced liver disease. The CT and TT genotypes are also associated with elevated viral load and increased fibrosis severity. Further large-scale investigations across diverse regions of Pakistan are required to comprehensively understand the role and association of TLR-3 SNPs with susceptibility to HCV infection and disease progression.
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Muhammad Anwar
Institute of Zoological Sciences University of Peshawar Pakistan
Dr. Sanaullah Khan
Institute of Zoological Sciences University of Peshawar Pakistan
Amina Gul
Department of Microbiology, Khyber Medical College Peshawar Pakistan
Muhammad Adnan
Institute of Zoological Sciences University of Peshawar Pakistan
Ata Ur Rehman
Institute of Biological Sciences, Gomal University, Dera Ismail Khan Pakistan