COMPARISION OF PCR CATRIDGE BASED WITH ROUTINE PROCEDURE FOR DIAGNOSIS OF PAEDIATRIC TUBERCULOSIS & HUMAN IMMUNO DEFICIENCY VIRUS

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Dr. B.S.V Sivanagaraja
Dr. S. Kalyan Kunchapudi

Keywords

Catridge, PCR, traditional techniques, diagnosing paediatric TB

Abstract

Introduction: Throughout ancient times, tuberculosis has posed a serious threat to humankind. Around 150 million years ago is when the Mycobacterium genus was first recognised. Egyptian mummies have been used to show the skeletal malformations caused by TB. Yet, the infectious agent behind this terrible sickness was not identified for quite some time.


Material and Methods: Between February 2022 and November 2022, the department of Microbiology at the Maheshwara Medical College and Hospital in Patancheru, Telangana conducted a prospective study. A total of one hundred people were included as part of this study's sample.


Results: Children who had expressed concerns were asked for and given permission to have a sample taken by their parents or legal guardians. Gastric aspirates, broncho-alveolar lavage, induced sputum, sputum, tracheal aspirate, ascitic fluid, lymph node aspirates, pleural fluid, and a synovial biopsy were all included in the collection of specimens. There was complete processing and analysis of all samples.


Conclusion: This research was conducted to identify the most efficacious techniques for detecting TB in young people. CBNAAT has an advantage over other methods used to diagnose paediatric TB because it picked up more instances that were overlooked by those other approaches.

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References

1. World Health Organization, editor. Global tuberculosis report 2017. World Health Organization; 2017.
2. Marais BJ, Hesseling AC, Gie RP, Schaaf HS, Beyers N. The burden of childhood tuberculosis and the accuracy of community- based surveillance data. Int. J. Tuberc. Lung Dis. 2006;10(3) : 259-63.
3. Kabra SK, Lodha R, Seth V. Some current concepts on childhood tuberculosis. Indian J Med Res. 2004;120(4) : 387-97.
4. B. J. Marais and M. Pai, “Recent advances in the diagnosis of childhood tuberculosis,” Archives of Disease in Childhood, vol.92, no. 5, pp. 446–452, 2007.
5. S. H. Montenegro, R. H. Gilman, P. Sheen et al., “Improved detection of Mycobacterium tuberculosis in Peruvian children by use of a heminested IS6110 polymerase chain reaction assay.” Clin Infect Dis.2003; 36(1):16–23.
6. Bennett JE, Dolin R, Blaser MJ. Mandell, Douglas and Bennett’s Infectious Disease Essentials E-Book. Elsevier Health Sciences; 2016 Feb 25.
7. Idigoras P, Perez-Trallero E, Alcorta M, Gutierrez C, Munoz- Baroja I. Rapid detection of tuberculous and nontuberculous mycobacteria by microscopic observation of growth on Middlebrook 7H11 agar. Eur J Clin Microbiol Infect Dis. 1995; 14 : 6-10.
8. Ninan SA. Comparative study of different methods of identification of Mycobacterium tuberculosis in gastric aspirate of children suffering from pulmonary tuberculosis. MD thesis. All India Institute of Medical Sciences, New Delhi; 1997.
9. Seagar AL, Neish B, Laurenson IF. Comparison of two in-house real-time PCR assays with MTB Q-PCR Alert and GenoType MTBDRplus for the rapid detection of mycobacteria in clinical specimens. J Med Microbiol. 2012 Oct 1;61(10):1459-64.
10. Zignol M, Sismanidis C, Falzon D, Glaziou P, Dara M, Floyd K. Multidrug-resistant tuberculosis in children: evidence from global surveillance. Eur Respir J. 2013 Sep 1;42(3):701-7.
11. Kapur, V., Whittam, T. S., & Musser, J. M. Is Mycobacterium tuberculosis 15,000 years old? J Infect Dis.1994;170(5):1348-49.
12. Seibert, F. B. "The isolation and properties of the purified protein derivative of tuberculin." Am Rev Tuberc. 1934;30(Suppl):713-20.
13. Iseman, M. "Tuberculosis therapy: past, present and future."Eur Respir J. 2002 ; 20 (36 suppl): 87-94.
14. E. A. Khan and J. R. Starke, “Diagnosis of tuberculosis in children: increased need for better methods,” Emerg Infect Dis, vol. 1, No. 4, pp. 115–123, 1995.
15. S. M. Graham, R. P. Gie, H. S. Schaaf, J. B. S. Coulter, M.A. Espinal, and N. Beyers, “Childhood tuberculosis: clinical research needs,” Int J Tuberc Lung. Dis, vol. 8, no. 5, pp. 648–657, 2004.
16. Lotfian F, Bolursaz MR, Tabarsi P, Velayati A. Comparison Between Pulmonary and Extrapulmonary Tuberculosis in Adolescents. Arch Pediatric Infect Dis. 2017; 5(3):e57253.
17. Devrim I, Aktürk H, Bayram N, Apa H, Tulumoğlu Ş, Devrim F, Erdem T, Gulfidan G, Ayhan Y, Tamsel İ, Can D. Differences between pediatric extra-pulmonary and pulmonary tuberculosis: a warning sign for the future. Mediterr J Hematol Infect Dis 2014; 6; Open Journal System.
18. De D, Kinikar A, Adhav PS, Kamble S, Sahoo P, Koli H, Kanade S, Mave V, Suryavanshi N, Gupte N, Gupta A. Source case investigation for children with TB disease in Pune, India. Tuberculosis research and treatment. 2014; 27:182836
19. Vijayasekaran D, Kumar RA, Gowrishankar NC, Nedunchelian K, Sethuraman S. Mantoux and contact positivity in tuberculosis. Indian J Pediatr. 2006;73(11):989-93.
20. Osborne CM. The challenge of diagnosing childhood tuberculosis in a developing country. Arch Dis Childhood. 1995;72(4):369.
21. Annam V, Kulkarni MH, Puranik RB. Comparison of the modified fluorescent method and conventional Ziehl-Neelsen method in the detection of acid fast bacilli in lymph node aspirates. Cyto journal. 2009; 6: 13
22. Hooja S, Pal N, Malhotra B, Goyal S, Kumar V, Vyas, L. Comparison of Ziehl-Neelsen & Auramine O staining methods on direct and concentrated smears in clinical specimens. Indian J Tuberc.2011; 58: 72-6.
23. Zaib-Un-Nisa, Javed H, Zafar A, Qayyum A, Rehman A, Ejaz H. Comparison of fluorescence microscopy and Ziehl-Neelsen technique in diagnosis of tuberculosis in paediatric patients. J Pak Med Assoc.2015;65(8):879-81.
24. Steingart KR, Henry M, Ng V, Hopewell PC, Ramsay A, Cunningham J, Urbanczik R, Perkins M, Aziz MA, Pai M. Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review. The Lancet infectious diseases. 2006 Sep 30;6(9):570-81.
25. Laifangbam S, Singh HL, Singh NB, Devi KM, Singh NT. A comparative study of fluorescent microscopy with Ziehl-Neelsen staining and culture for the diagnosis of pulmonary tuberculosis. Kathmandu University Medical Journal. 2009;7(3):226-30.
26. Agrawal M, Bajaj A, Bhatia V, Dutt S. Comparative study of GeneXpert with ZN stain and culture in samples of suspected pulmonary tuberculosis.J Clin Diagn Res. 2016;10(5): DC09.
27. S. Iram, Hussain S. ZeenatA, N.W. Yusuf, M. Aslam, Rapid diagnosis of tuberculosis using Xpert MTB/RIF assay–report from a developing country, Pak. J. Med. Sci. 31 (1) (2015) 105–10.
28. Bajrami R, Mulliqi G, Kurti A, Lila G, Raka L. Comparison of GeneXpert MTB/RIF and conventional methods for the diagnosis of tuberculosis in Kosovo. J Infect Dev Ctries. 2016;10(04) : 418
29. Fairlie L, Beylis NC, Reubenson G, Moore DP, Madhi SA. High prevalence of childhood multi-drug resistant tuberculosis in Johannesburg, South Africa: a cross sectional study. BMC Infect Dis. 2011;11(1):28.
30. Kassa-Kelembho E, Bobossi-Serengbe G, Takeng EC, Nambea- Koisse TB, Yapou F, Talarmin A: Surveillance of drug-resistant childhood tuberculosis in Bangui, Central African Republic. Int Tuberc Lung Dis. 2004, 8 (5): 574-8.