TRACHYSPERMUM AMMI AND CITRUS LIMON AMALGAM: A GATEWAY TO THE NATURAL MEDICATION

Main Article Content

Asma Irshad
Aisha Tahir
Ansar Zubair
Mehreen Fatima
Muhammad Aurangzaib

Keywords

Antimicrobial compounds, antioxidant activity, organic extracts, phytomedicinal plants

Abstract

Background and Aim: Trachyspermum ammi and Citrus limon as strong phytomedicinal plants are frequently utilized in the treatment of a range of illnesses. The present research is designed to analyze the antioxidant and antimicrobial activities of Trachyspermum ammi (ajwain) and Citrus limon (lemon) of distilled water, acetone, ethanol, and methanol against Salmonella typhi and Staphylococcus aureus by utilizing the Well diffusion technique.


Methods: The microdilution process was performed to determine the minimum inhibitory concentration (mg/mL ± SD) of both the selected plant extracts. The antioxidant activity of Trachyspermum ammi and Citrus limon was examined via total phenolic contents (mg/g), total flavonoid contents (mg/g), DDPH (%), and reducing power (nm), the results were explicated by applying ANOVA.


Results: The antimicrobial examination discovered that Citrus limon produced the highest zone of inhibition in methanol (7.3±0.05mm ± SD) against Staphylococcus aureus whereas for Salmonella typhi, aqueous (13.6±0.5mm ± SD) produced highest activity among the other organic extracts. Correspondingly, Trachyspermum ammi provided a maximum zone of inhibition in methanol (20.3±0.58mm ± SD) against Staphylococcus aureus and for Salmonella typhi, ethanol (22.3±0.58mm ± SD) presented maximum activity. The highest total phenolic content was found in the acetone extract of lemon (11.0±0.57mg/g ± SD), however, the lowest was in distilled water (0.197±1.25mg/g ± SD) of ajwain. The total flavonoid content was analyzed to be the greatest in acetone of lemon (6.23±0.30mg/g ± SD) however the lowest in acetone of ajwain (0.038±0.80mg/g ± SD). Likewise, lemon and ajwain presented notable DPPH radical scavenging activity and reducing potential.


Conclusion: Consequently, Trachyspermum ammi and Citrus limon extracts have medicinal potential and can be exploited in the pharmaceutical industry.

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References

1. Ilić, Z.S., Milenković, L., Tmušić, N., Stanojević, L., Stanojević, J., & Cvetković, D., Essential Oils Content, Composition and Antioxidant Activity of Lemon Balm, Mint and Sweet Basil from Serbia. LWT 153 2022: 112210.
2. Ökmen, G., Arslan, K., & Tekin, R. C., The Antimicrobial Activity of Citrus Limon L. Against Foodborne Pathogens and Its Anti-Oxidant and Antibiofilm Properties. 2021.
3. Krebs-Holm, L., Lemons: Health Benefits, Nutrition, and Side Effects January 2, 2020 - Updated on November 10, 2020 eMedihealth, 2020.
4. Asker, M., et al., Phytochemical constituents and antibacterial activity of Citrus lemon leaves. Bulletin of the National Research Centre, 2020. 44(1): p. 1-7.
5. Heben, D., 16 Health Benefits of Lemon Seeds Dr.HealthBenefits.com, 2020.
6. Jothiramalingam, R., Devasanan, S., Lohedan, H. A., Muthumareeswaran, M. R., Alqahtani, H. M., & Abdalnaser, K., Green Chemistry Method Prepared Effective Copper Nanoparticles by Lemon Flower (Citrus) Extract and Its Anti-Microbial Activity. Dig. J. Nanomater. Biostr 17 (2022): 145-51.
7. Presentato, A., et al., Superior antibacterial activity of integral lemon pectin extracted via hydrodynamic cavitation. ChemistryOpen, 2020. 9(5): p. 628.
8. Kumar, A., & Singh, A. K., Trachyspermum Ammi (Ajwain): A Comprehensive Review. WJPR 10 (2021): 724-36.
9. Lu, S.Y., Chu, Y. L., Sridhar, K., & Tsai, P. J., Effect of Ultrasound, High-Pressure Processing, and Enzymatic Hydrolysis on Carbohydrate Hydrolyzing Enzymes and Antioxidant Activity of Lemon (Citrus Limon) Flavedo. LWT 138 (2021): 110511.
10. Mayekar, V.M., Ali, A., Alim, H., & Patel, N., A Review: Antimicrobial Activity of the Medicinal Spice Plants to Cure Human Disease. Plant Science Today 8, no. 3 (2021): 629–46-29–46.
11. Asif, H.M., H.A.S.J.B.C.i.U.V. Hashmi, and Legumes, Bioactive Compounds of Ajwain (Trachyspermum ammi [L.] Sprague). 2021: p. 257-273.
12. DeNoon, D.J., Health Benefits of Ajwain. webMD, 2020.
13. Nouri Gharajalar, S., Antibacterial Activity of Ajwain Essential Oil against Some Zoonotic Bacteria. Journal of Zoonotic Diseases 5, no. 2 (2021): 26-33.14.
14. Arif, S., et al., Comparison of Nigella sativa and Trachyspermum ammi via experimental investigation and biotechnological potential. Chemical Engineering and Processing-Process Intensification, 2021. 161: p. 108313.
15. Nguta, J.M. and M.N. Kiraithe, In vitro antimicrobial activity of aqueous extracts of Ocimum suave Willd., Plectranthus barbatus andrews and Zanthoxylum chalybeum Engl. against selected pathogenic bacteria. Biomedical and Biotechnology Research Journal (BBRJ), 2019. 3(1): p. 30.
16. Kulkarni, S.S., Mishra, S., Patil, S. B., Nambiar, J., & Math, A., Unifloral Ajwain Honey Ameliorates Differential Inhibition of Matrix Metalloproteinases 2 and 9 Protein, Cytotoxicity, and Antioxidant Potential. Journal of Ayurveda Integrative Medicine 12, no. 4 (2021): 633-39.
17. Khandker, S.S., Kabir, A., Hasan, M., Ahmed, M., Gan, S. H., Khalil, M., ... & Kamal, M. A., Elachi Lemon (Citrus Limon) Peel and Pulp: Antioxidant, Antimicrobial, Anticoagulant Activities, Bioactive Compounds, Minerals, and Heavy Metals. Current Bioactive Compounds 17, no. 6 2021: 47-58.
18. Yazdeli, M.Z., et al., Evaluation of antibacterial synergism of methanolic extract of Dracocephalum kotschyi and Trachyspermum ammi. The Malaysian journal of medical sciences: MJMS, 2021. 28(6): p. 64.
19. Ardestani, M.M., et al., Evaluation of Antimicrobial Activity of Trachyspermum ammi (L.) Sprague essential oil and its active constituent, thymol, against vaginal pathogens. Traditional Integrative Medicine, 2020: p. 49-58.

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