ANALYTICAL ESTIMATION AND VALIDATION OF LAMOTRIGINE BY NEW HPLC METHOD
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Keywords
Lamotrigine, Method Development, Validation, HPLC
Abstract
A simple, rapid, reproducible and precise method was developed for the quantitative determination of lamotrigine in its pure form as well as in pharmaceutical dosage forms. The method is based on high performance liquid chromatography. Alliance Waters e2695 Separations Module with Waters 2774 pump HPLC system equipped with autosampler, ultraviolet detector, and Waters Atlantis T3 C-18 5µm, 4.6 X 150 mm column were used for the quantification of the drug. Separation was carried out at a flow rate of 0.7 mL/min. of mobile phase (acetonitrile and ethanol) and the detection was carried out at a wavelength of 275 nm. The lamotrigine was well resolved on the stationary phase and the method was validated and shown to be linear for lamotrigine in the range of 5-25 μg/ml. The method was validated for Precision, Accuracy, LOD and LOQ were determined and the correlation coefficient for lamotrigine was found to be 0.9901 respectively. The developed method was found to be repeatable and reproducible; hence, it can be used as an alternative method in assay of the lamotrigine in any pharmaceutical industries
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Pallavi Joshi
PhD Scholar, Department of Pharmaceutics, School of Pharmaceutical Sciences, SGRR University
Patel Nagar, Dehradun, Uttarakhand, India
Ashutosh Badola
Department of Pharmaceutics, School of Pharmaceutical Sciences, SGRR University
Patel Nagar-248001, Dehradun, Uttarakhand, India