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Dr. Muhammad Sulaman Pirzado
Meera Kumari
Iram Shaikh
Sikander Munir Memon


Psoriasis, Histamine H4 Receptor, Gene Expression, HRH4, Biomarker, Sindh, Pakistan


Background: Psoriasis is a chronic inflammatory skin disorder with a complex pathophysiology involving a wide array of immune responses. The Histamine H4 Receptor (H4R) has been identified as a potential mediator in inflammatory responses, with its role in psoriasis yet to be fully elucidated. This study investigates the expression levels of the Human Histamine H4 Receptor Gene (HRH4) in psoriasis patients in Sindh, Pakistan, aiming to understand its association with disease status, severity, and treatment history.

Methods: This cross-sectional study was conducted across multiple locations in Sindh, Pakistan. A total of 200 participants, comprising both psoriasis patients and healthy controls, were enrolled. Blood samples were collected for RNA extraction and subsequent cDNA synthesis. Polymerase Chain Reaction (PCR) was utilized to assess HRH4 gene expression. Participants were categorized based on disease severity, treatment history, and clinical characteristics. Statistical analysis included Independent Samples T-Test and ANOVA to compare gene expression levels across different groups.

Results: The study found a statistically significant difference in HRH4 gene expression between controlled (mean Ct value = 2.609) and uncontrolled psoriasis patients (mean Ct value = 4.867, p < 0.001). Higher gene expression levels were associated with uncontrolled disease status and more severe disease, as indicated by higher Psoriasis Area and Severity Index (PASI) scores. Furthermore, patients with a history of treatment showed higher gene expression levels compared to those without any treatment.

Conclusion: The HRH4 gene expression levels in psoriasis patients vary significantly with disease control status and severity, suggesting that HRH4 could serve as a potential biomarker for disease activity and therapeutic response. This study highlights the importance of gene expression profiling in understanding psoriasis pathophysiology and underscores the need for further research to explore the therapeutic potential of targeting HRH4 in psoriasis treatment.


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