Evaluation of Antibacterial Effect of Ethanolic Grapes Seed Extract and Sodium Hypochlorite as Root Canal Irrigants using Two Different Techniques in Primary Molars: An in-Vitro Study
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Keywords
E.faecalis , S.mutans , Sodium Hypochlorite (NaOCl) , Grape seed extract (GSE) , passive ultrasonic irrigation (PUI) , conventional syringe irrigation (CSI) .
Abstract
It is critical to preserve deciduous teeth near the time of their shedding. Pulpectomy is one therapy option for achieving this goal. The root canal system complexity of the deciduous teeth allows pathogenic microorganisms to spread thorough, making elimination of necrotic tissue using mechanical instrumentation alone unfeasible. The objective of this study was to evaluate and compare the antibacterial effect of 13% Ethanolic grape seeds extract (GSE) versus 5.25% Sodium Hypochlorite (NaOCl) solutions using conventional syringe irrigation(CSI) and passive ultrasonic irrigation (PUI) techniques in primary molars .
Methods: 60 unidentified extracted human primary mandibular first molars with at least 2/3 of root not resorbed. No root caries. No internal or external resorption. (confirmed by periapical X- ray) .Teeth with no signs of visible root cracks confirmed by careful examination . No anatomic abnormalities as fusion or dilacerations. This study focused only on distal roots, so decoronation was performed on all distal roots below the cementoenamel junction until the root length reached 8 mm. 60 prepared distal roots were divided randomly into 2 main groups according to infected bacteria 30 roots infected with E. facials (standard cultures ATCC 29212) and 30 roots infected with S.mutans (standard Cultures ATCC 27351), then each group sub-divided into 5 subgroups according to irrigation technique and types of irrigants: no irrigation (control), CSI technique with 5.25%NaOCl (positive control) ,CSI technique with13% Ethanolic GSE solution. PUI with 5.25% NaOCl. PUI with 13% Ethanolic GSE solution. Results: NaOCl 5.25% had a significantly stronger antibacterial impact than GSE 13% (p<0.05). In this aspect, no significant differences were observed between the impacts of the CSI and PUI techniques in the E. faecalis and S. mutans groups. Conclusion: A 13% GSE solution showed an antibacterial effect against E. faecalis and S. mutans, but to a lesser extent than a 5.25% NaOCl solution. Within the limits of the results of this study, it was found that 5.25% NaOCl solution is the best in antibacterial effect. It was also found that there is no significant difference in the results when using CSI or PUI technique
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