A STUDY TO EVALUATE EFFECTIVENESS OF THE ANTICANCER PROPERTY OF ASPIRIN AND IBUPROFEN IN MCF-7 BREAST CANCER CELL LINES.
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Keywords
Aspirin, Ibuprofen, Anti-cancer property, MCF 7 cell line, Breast cancer
Abstract
Background: Breast cancer is a leading cause of morbidity and mortality among women worldwide. Insights employing MCF-7 cell lines for breast cancer research have continued unabatedly in the last five decades. The MCF-7 cell line has radically changed the trajectory of breast cancer research and helped to improve patient outcomes, despite the drawbacks of research on established cancer cell lines maintained in tissue culture and xenografts. NSAIDs, including Aspirin and Ibuprofen, have been associated with a reduced risk of breast cancer.
Objectives: The present study was undertaken as a pre-clinical cross-sectional study to evaluate and compare the anti-cancer properties of Aspirin and Ibuprofen in MCF 7 breast cancer cell lines.
Methodology: 3-(4,5- dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, DAPI (4,6 Diamidino-2-phenylindole dihydrochloride) staining, Annexin assay and DNA fragmentation by Gel electrophoresis were employed in the study.
Results: IC50 values for Ibuprofen (28 μg/ml) were higher than Aspirin (21.18 μg/ml). No significant difference in the percentage of cancer cell death between Aspirin and Ibuprofen was noted. When combined, Aspirin and Ibuprofen showed significantly higher anti-cancer properties (IC50-14.93 μg/ml) on breast cancer cell lines compared to their standalone samples. Apoptosis level by annexin assay was increased at 15μg of Aspirin and Ibuprofen. DAPI staining also showed that a greater number of cancer cells were stained at 15μg of Aspirin and Ibuprofen. Fragmentation of the combination drugs of 10μg shows the highest apoptosis from control through DNA ladder Assay.
Conclusion: Thus, Aspirin and Ibuprofen, exclusively and in combination, have been found to have anti-cancer properties against breast cancer cell lines through various laboratory methods.
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