UNRAVELLING THE LINK BETWEEN ROS AND MALE FERTILITY: HOW EXCESSIVE PRODUCTION CAN IMPACT LIPID PEROXIDATION

Main Article Content

Rajendra Kumar Sahoo
Babita Panda
Jeetendra Behera
Priyanka Parida
Avijit Panda

Keywords

Reactive Oxygen Species (ROS), Male Fertility, Lipid Peroxidation, Oxidative Stress, Reproductive Health.

Abstract

Objective: Empirical research will be undertaken to establish a correlation between Reactive Oxygen Species (ROS) and male fertility, using the approach of characterization of the damaging effects of increased ROS levels on sperm lipid oxidation in humans. Methodology: Human sperm were obtained from patients, and in vitro experiments were conducted to determine the impact of ROS concentration on the process of lipid peroxidation. ROS levels were regulated by means of aggressive environment with time gaps, and lipid peroxidation markers were analysed with the help of easy-to-do biochemical tests. Results: We found that oxidative stress, which results in exceeding ROS levels triggered by the laboratory experiments, demonstrated a positive connection between ROS levels and sperm cells. The spermatozoon exposed to higher ROS showed structural damage of the membrane and the decrease in both sperm motility and vitality. The processes, used in scientific experimentation, demonstrated the role of pro-oxidant species in human sperm dynamics. Conclusion: With the conclusion of the research, a straight line is associated between ROS, lipid peroxidation and male fertility can be drawn in the context of a human being. The results provide us with hints on searching for novel approaches realizing the purpose of alleviating oxidative stress in male germ cells that might be followed by the developments of therapeutic approaches which have the same goal as the one improving male fertility.

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