QUANTITATIVE ANALYSIS OF HYDROETHANOLIC EXTRACT OF MORINGA OLIEFERA BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC)
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Keywords
HPTLC, Hydroethanolic extract, Phytochemical profiling, Moringa oliefera
Abstract
Objective: Moringa oliefera is well known for its therapeutic properties because of presence of bioactive compounds. Therefore, in the present study, the High-Performance Thin Layer Chromatography (HPTLC) analysis of hydroethanolic extract of Moringa oliefera was performed for its phytochemical profiling.
Methods: Preliminary phytochemical screening was done. HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, Reprostar 3 and WIN CATS were used. The HPTLC densitometric analysis of the hydroethanolic extract of Moringa oliefera was carried out using CAMAG HPTLC system, and the results were obtained in the form of chromatograms (scanned at the wavelength of 254 nm and 366 nm) representing several peaks.
Results: Preliminary phytochemical screening of the extract showed the presence of alkaloids, flavonoids, saponins, tannins, terpenoids, phenolic compounds, proteins. HPTLC finger printing of hydroethanolic extract of Moringa oleifera under white light reveal presence of seven components 0.04, 0.05, 0.25, 0.29, 0.40, 0.44, 0.96. Component number 2,3 and 5 at Rf 0.05, 0.25 and 0.40 respectively showed maximum concentration.
Conclusion: The study came to the conclusion that Moringa oliefera's hydroethanolic extract includes a wide range of phytochemicals that may be responsible for its medicinal potential, supporting the plant's traditional use in India.
References
2. Patel P, Patel N, Patel D, Desai S, Meshram D (2014) Phytochemical Analysis and Antifungal Activity of Moringa Oleifera International Journal of Pharmacy and Pharmaceutical Sciences Vol 6, Issue 5.
3. Cooper LN, Blais BS (2004) Theory of cortical plasticity. World scientific publishing, Singapore
4. N. Malliga Elangovan, M.S. Dhanarajan, I. Elangovan (2015) Preliminary Phytochemical Screening and HPTLC Fingerprinting Profile of Leaf Extracts of Moringa oleifera and Phyllanthus emblica. International Research Journal of Pharmaceutical and Biosciences (IRJPBS) 2 (2) 32- 40.
5. Mandrika I, Kumar S, Zandersone B, Eranezhath SS, Petrovska M, Liduma I, Jezupovs A, Pirags V and Tracevska T (2021) Antibacterial and Anti-Inflammatory Potential of Polyherbal Formulation Used in Chronic Wound Healing; Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2021, 13 pages.
6. Tilburt JC, Kaptchuk TJ (2008) Herbal medicine research and global health: an ethical analysis. Bull World Health Organ 86:594–599
7. Sisodiya D, Shrivastava P (2017) Qualitative and quantitative estimation of bioactive compounds of Euphorbia thymifolia L. Asian J Pharm Edu Res 6(3):34–43
8. Kokate CK. Practical Pharmacognosy. Vallabh Prakashan, New Delhi. 2005; 4th Edition: 106-109.
9. Khandelwal KR. Practical Pharmacognosy. Nirali Prakashan, Pune. 2007; 16th Edition: 149-156.
10. Tiwari, P., Kumar, B., Kaur, M., Kaur, G. and Kaur, H., 2011. Phytochemical screening and extraction: a review. Internationale pharmaceutica sciencia, 1(1), pp.98-106.
11. Singh GK, Bhandari A. Text book of Pharmacognosy. CBS Publisher & Distributor, New Delhi. 2005; 1st Edition: 39-62.
12. Gupta RC, Bhargava S. Practical Biochemistry. CBS Publisher & Distributor New Delhi. 2006; 4th Edition: 10-44.
13. Lehman, J. W. The student's lab companion: laboratory techniques for organic chemistry: standard scale and microscale. Pearson College Div, (2008).
14. Pavia, D. L., Lampman, G. M., Kriz, G. S., & Engel, R. G. Microscale and Macroscale Techniques. Thomson Wadsworth, (2006).
15. Pannkuk, E. L., Risch, T. S., Savary, B. J. (2013) Profiling the Triacyl glyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry. J. Vis. Exp. (79), e50757.
16. Kagan, I. A., Flythe, M. D. (2014) Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds. J. Vis. Exp. (85), e51411.
17. Egon Stahl, TLC- A laboratory handbook, 2nd edition, international student edition, New York, 346-349, 421-461.
18. Wagner H. and Bladt S, Plant Drug Analysis -and A Thin Layer Chromatographic Atlas, Springer- Verlag Berlin Heidelberg, 1996.
19. Harborne, J.B., Phytochemical methods, 3rd edition, Chapman and Hall, London, 1998.
20. Fatemeh Fathiazada, Abbas Delazara, (2006) Extraction of Flavonoids and Quantification of Rutin from waste Tobacco Leaves, Iranian Journal of Pharmaceutical Research 3: 222-227.
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Sushma Rathod
Research scholar, Institute of Pharmacy, Oriental University Indore MP
Dr. Mohini Sihare
Professor, Faculty of Pharmacy, Oriental University Indore MP