EVALUATION OF THE TOXICITY, ANTIOXIDANT, AND ANTIMITOTIC IMPACT OF MORINGA OLEIFERA USING IN-VITRO, EX-VIVO, AND IN-VIVO MODELS.
Main Article Content
Keywords
moringa oleifera, antimitotic activity, phenols, terpenoids, tannins, glycosides, saponins
Abstract
Moringa Oleifera, a tropical plant, is characterized by its noteworthy nutritional and medicinal attributes. The present study involved the extraction of Moringa Oleifera leaves utilizing the n-hexane solvent. Employing this methodology, a preliminary investigation of phytochemical constituents, organic functional group identification, toxicological assessment through brine shrimp and chicken eye experiments, and antimitotic evaluation using earthworms were conducted. Phenols Test
The phytochemical screening indicated the presence of terpenoids, fats, steroids, tannins, glycoside and phenols in the n-hexane extract of moringa oleifera bark. Similarly, the n-hexane extract of moringa oleifera leaves exhibited positive results for phenols, terpenoids, tannins, glycosides, saponins, and fats. Infrared spectral data analysis revealed distinctive bonds in the extracts, with the leaves extract exhibiting C-H, C=O, C=C, C-C, and C-O bonds indicative of aliphatic and aromatic compounds. Conversely, the bark extract showcased N-H, O-H, C=O, C=C, and N-O bonds, suggesting the presence of aromatic or aliphatic phenols, alcohols, and nitrogen-containing compounds.
The brine lethality assay demonstrated a proportional increase in lethality with escalating extract concentrations. However, no ocular toxicity was observed in the chicken eye experiments. Notably, the hexane extract of moringa oleifera leaves exhibited pronounced antimitotic activity, underscoring its potential application in cancer treatment due to this specific biological activity.
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21. Jung, I. L. (2014). Soluble extract from Moringa oleifera leaves with a new anticancer activity. PloS one, 9(4), e95492.
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Pravin D Badhe, Pranali D Bangaiya, Rutuja N Mule, Prashant Mali, Rutuja M Shinde, Swarda V Kamble, Leena Pathak, & Ashwini P Badhe. (2024). EVALUATION OF THE TOXICITY, ANTIOXIDANT, AND ANTIMITOTIC IMPACT OF MORINGA OLEIFERA USING IN-VITRO, EX-VIVO, AND IN-VIVO MODELS. Journal of Population Therapeutics and Clinical Pharmacology, 31(2), 562–579. https://doi.org/10.53555/jptcp.v31i2.4416
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Author Biographies
Pravin D Badhe
Sinhgad College of Pharmacy, Vadgaon (BK) Pune Maharashtra India
Pranali D Bangaiya
Sinhgad College of Pharmacy, Vadgaon (BK) Pune Maharashtra India
Rutuja N Mule
Sinhgad College of Pharmacy, Vadgaon (BK) Pune Maharashtra India
Prashant Mali
Sinhgad College of Pharmacy, Vadgaon (BK) Pune Maharashtra India
Rutuja M Shinde
Sinhgad College of Pharmacy, Vadgaon (BK) Pune Maharashtra India
Swarda V Kamble
Sinhgad College of Pharmacy, Vadgaon (BK) Pune Maharashtra India
Leena Pathak
Jaihind College of Pharmacy, vadgaon Sahani, Junnar, Pune
Ashwini P Badhe
Swalife Biotech Ltd, Ireland