MOLECULAR CHARACTERIZATION OF GRASSHOPPER SPECIES FROM TEHSIL OGHI

Main Article Content

Rehana Shaheen
Zeeshan Abbas
Muhammad Nabeel
Dr.Razia Virk
Fatima Habib
Sarmad Ali shah
Eaman Naveed

Keywords

Abstract

Grasshopper belongs to the order Orthoptera, under the class Insecta. Grasshoppers are pests of vegetation and crops. The research was conducted on the molecular study and phylogenetic relationship of collected species of Grasshoppers, within families Tettigoniidae, Acrididae, and Dericorythidae, collected from tehsil, oghi. A total of 70 samples was collected and morphological identification was done using an Orthopteran Taxonomic key. The taxonomic analysis revealed 9 species, under 8 genera, and spreading into 3 families. The 9 identified species revealed i-e Melanoplus bivittatus, Anacridium aegyptium, hieroglyphs Banian, Kosciuscola tristis tristis, stenobothrus stigmatics, under the family Acrididae. Two species i-e Omocestus viridulus and Conocephalus fuscus belong to the family Tettigoniidae. While the two species i-e Dericorys albidula, Dericorys tibialis resulted under the family Dericorythidae. DNA was isolated from Grasshoppers' legs using the Gervay protocol. The amplification of 16 Sr RNA was performed through polymerase chain reaction (PCR). Amplified 16 Sr RNA gene was set for sequencing. After successful sequencing,   samples were further identified by BLAST in the Gene Bank, NCBI. The molecular study was performed with various parameters i-e Genetic distances, Molecular Clock, and Phylogenetic relationship. The genetic distance was estimated to be 0.168-0.187. The value of the molecular clock revealed 0.168. The phylogenetic tree was based on the Neighbor-Joining tree, the tree length was revealed 0.00 to 0.021. The evolutionary relationship among collected species and these species retrieved from GeneBank were clustered together and their similarities were recorded. The estimated phylogenetic tree of the species was also clustered based on the Neighbor-Joining tree. The samples collected and processed contain no novel species; all of the species are pre identified but first time recorded from tehsil oghi.

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