EFFECTS OF ZINC OXIDE (ZNO) NANOPARTICLES ON HISTOPATHOLOGY OF KIDNEY AND HEMATOLOGICAL PARAMETER OF ALBINO RAT
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Keywords
Zinc Oxide Nanoparticles, Albino Rat, Hematological Parameter of Rat
Abstract
Zinc oxide nanoparticles (ZnO NPs) are widely prevalent in many facets of human life and have been used in a variety of sectors. As a result, questions have been raised regarding their potential negative effects. Although ZnO NP toxicity is well known, the effects of nanoparticle exposure are yet completely unknown. The present study is to evaluate the effects of zinc oxide nanoparticles on the hematological parameter and histopathology of kidney of rat. For this purpose twelve male albino rats were taken and acclimatized under laboratory condition for a week. After the acclimatized period the rats were divided into three groups. The control group T1 was treated with no nanoparticles. The T2 was treated with 50mg/kg and T3 was treated with 100mg/kg zinc oxide nanoparticles. After three weeks of experimental period, the blood was drowned from the heart of rats. Results illustrated that in hematological analysis the count of RBCs HB and platelets were decreased in treatment (T2) and (T3) as compared to (T1). Maximum decreased was found in T3 with mean value of 3.68+0.23, 8.75+0.19 and 173.25+15.52 and minimum decreased was observed in T2 with mean value of 4.13+0.11, 10+0.24 and 181+10.48 for RBCs, HB and platelets. While WBCs levels increased in T2 and T3 with mean values of 21.05+0.50 and 2.52+0.25 respectively. From these results, we can conclude that histopathological showed necrosis and detachment from basal membrane of kidney and congestion was also observed at some places.
References
2. S.P. Forster, S. Olveira, and S. Seeger. Nanotechnology in the market: promises and realities. International Journal of Nanotechnology 8:592-613 (2011).
3. F. Piccinno, F. Gottschalk, S. Seeger, and B. Nowack. Industrial production quantities and uses of ten engineered nanomaterials in Europe and the world, Journal of Nanoparticle Research 14:1-11 (2012).
4. J.C. Chow, J.G Watson, N. Savage, C.J. Solomon, Y.S. Cheng, P.H. McMurry, L.M. Corey, G.M. Bruce, R.C. Pleus, R.C. Pleus, and P. Biswas. Nanoparticles and the environment. Journal of the Air and Waste Management Association 55:1411-1417 (2005).
5. L. Tang, and P. Shapira. China-US scientific collaboration in nanotechnology: patterns and dynamics. Scientometrics 88:1-16 (2011).
6. S. Hasan. A review on nanoparticles: their synthesis and types. Research journal of recent sciences 2277: 2502-2503 (2015).
7. E. Baranowska-Wójcik, D. Szwajgier, P. Oleszczuk, and A. Winiarska-Mieczan. Effects of titanium dioxide nanoparticles exposure on human health. Biological trace element research 193:118- 129 (2020).
8. D. Cruz, P.L. Falé, A. Mourato, P.D. Vaz, M.L. Serralheiro, and A.R.L. Lino. Preparation and physicochemical characterization of Ag nanoparticles biosynthesized by (Lemon) Verbena) Lippia citriodora. Colloids and Surfaces B: Biointerfaces 81:67-73 (2010).
9. S. Sabir, M Arshad, and S.K. Chaudhari. Zinc oxide nanoparticles for revolutionizing agriculture: synthesis and applications. Scientific World Journal 2014:1-8 (2014).
10. K.S. Siddiqi, A. ur Rahman, and A. Husen. Properties of zinc oxide nanoparticles and their activity against microbes. Nanoscale research letters 13:1-13 (2018).
11. M. Ghosh, S. Sinha, M. Jothiramajayam, A. Jana, A. Nag, and Mukherjee, A. Cyto-genotoxicity and oxidative stress induced by zinc oxide nanoparticle in human lymphocyte cells in vitro and Swiss albino male mice in vivo. Food and Chemical Toxicology 97: 286-296 (2016).
12. R. Chen, L. Huo, X. Shi, R. Bai, Z. Zhang, Y. Zhao, Y. Chang and C. Chen. Endoplasmic reticulum stress induced by zinc oxide nanoparticles is an earlier biomarker for nanotoxicological evaluation. ACS nano 8:2562-2574 (2013).
13. R. Bengalli, M. Gualtieri, L. Capasso, C. Urani, and M. Camatini. Impact of zinc oxide nanoparticles on an in vitro model of the human air-blood barrier. Toxicology letters 279:22- 32 (2017).
14. J. Liu, X. Feng, L. Wei, L. Chen, B. Song, and L. Shao. The toxicology of ion-shedding zinc oxide nanoparticles. Critical Reviews in Toxicology 46:348-384 (2016).
15. G. Schmalz, R. Hickel, K.L. Van Landuyt, and F.X. Reichl. Nanoparticles in dentistry. Dental materials journal 33:1298-1314 (2017).
16. D. Guo, H. Bi. B. Liu, Q. Wu, D. Wang, and Y. Cui. Reactive oxygen species-induced cytotoxic effects of zinc oxide nanoparticles in rat retinal ganglion cells. Toxicology in Vitro 27:731-738 (2013).
17. G.L. Bakris, N.A.N.C.Y. Lass, A.O. Gaber, J.D. Jones, and J.C Burnett Jr, J.C. Radiocontrast medium-induced declines in renal function: a role for oxygen free radicals. American Journal of Physiology-Renal Physiology 258:115-120 (2001).
18. P. Soderland, S. Lovekar, D.E. Weiner, D.R. Brooks, and J.S. Kaufman. Chronic kidney disease associated with environmental toxins and exposures. Advanced chronic kidney disease 17:254-264 (2010).
19. M.M. Brzoska, J. Moniuszko-Jakoniuk, B. Pilat-Marcinkiewicz, and B.Sawicki. Liver and kidney function and histology in rats exposed to cadmium and ethanol. Alcohol and Alcoholism 38:2-10 (2003).
20. A. So, and B. Thorens. Uric acid transport and disease. Journal of Clinical Investigation 120:1791-1799 (2010).
21. G. Oberdörster, E. Oberdörster, and J. Oberdörster. Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environmental health perspectives 113:823-839 (2005).
22. A.S.K. Heer. Spectral characterization and anti-fungal activity of zinc oxide (zno) nanoparticles synthesized using cynodon dactylon leaf extract. World Journal of Pharmaceutical Research 6:16 (2017).
23. M.D. Breyer, and Z. Qi. Better nephrology for mice—and man. Kidney international, 77(6): 487-489 (2010).
24. J. Fawcett, and J. Scott. A rapid and precise method for the determination of urea. Journal of clinical pathology 13(2): 156-159 (1960).
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Amara Akhtar
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan
Hina Fatima
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan
Ahmed Saqib
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan
Lala Rukh Bano
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan
Ammara
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan
Hafiza Asma Talib
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan
Rabia Khalid
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan